Abstract
Protein content and properties in the seminal plasma of Atlantic halibut (Hippoglossus hippoglossus) were assayed using spectrophotometric and electrophoretic methods. The protein concentration ranged from 6.4 ± 3.1 to 19.4 ± 3.4 mg ml−1 and anti-proteolytic activity from 585.2 ± 104.6 to 2912.4 ± 367.4 U l−l. A high correlation between anti-proteolytic activity and protein concentration (r = 0.95), and between sperm concentration and osmolality was found (r = 0.92). There was a significant decrease in anti-proteolytic activity from the first to the second sampling, but not in protein concentration. Anti-proteolytic activity and protein concentration were significantly affected by variations in individual males. Electrophoresis revealed four anti-proteolytic bands and individual differences in bands of proteolytic activity, which were subsequently characterized as metalloproteases and serine proteases.
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References
Babiak I, Ottesen O, Rudolfsen G et al (2006a) Quantitative characteristics of Atlantic halibut, Hippoglossus hippoglossus L., semen throughout the reproductive season. Theriogenology 65:1587–1604
Babiak I, Ottesen O, Rudolfsen G et al (2006b) Chilled storage of semen from Atlantic halibut, Hippoglossus hippoglossus L.: I. Optimizing the protocol. Theriogenology 66:2025–2035
Babiak I, Ottesen O, Rudolfsen G et al (2006c) Chilled storage of semen from Atlantic halibut, Hippoglossus hippoglossus L.: II. Effect of spermiation advancement, catheterization of semen, and production-scale application. Theriogenology 66:2036–2046
Ciereszko A, Dabrowski K, Lin F, Doroshov SI (1994) Identification of trypsin-like activity in sturgeon (Acipenser fulvescens) spermatozoa. Mol Reprod Dev 45:72–77
Ciereszko A, Liu L, Dabrowski K (1996) Effects of season and dietary ascorbic acid on some biological characteristics of rainbow trout (Oncorhynchus mykiss) semen. Fish Physiol Biochem 15:1–10
Ciereszko A, Piros B, Dabrowski K et al (1998) Serine proteinase inhibitor of seminal plasma of teleost fish: distribution of activity, electrophoretic profiles and relation to proteinase inhibitors of blood. J Fish Biol 53:1292–1305
Dabrowski K, Ciereszko A (1994) Proteinase inhibitor(s) in seminal plasma of teleost fish. J Fish Biol 45:801–809
Ellis A (1999) Immunity to bacteria in fish. Fish Shellfish Immunol 9:291–308
Geiger R, Fritz H (1983) Trypsin. Verlag Chemie, Weinheim
Griswold MD (1995) Interaction between germ cells and Sertoli cells in the testis. Biol Reprod 52:211–216
Haug T (1990) Biology of the Atlantic halibut, Hippoglossus hippoglossus (L.,1758). Adv Mar Biol 26:1–70
Kawabata C, Ichishima E (1997) Miltpain, new cystein protease from the milt of cum salmon, Oncorhynchus keta. Comp Biochem Physiol B Biochem Mol Biol 177:445–452
Kawabata C, Doi Y, Ichishima E (2000) Miltpain, a cysteine proteinase, from milt of Pacific cod (Gadus macrocephalus): purification and characterization. Comp Biochem Physiol B Biochem Mol Biol 125:533–542
Kowalski R, Glogowski J, Kucharczyk D et al (2003) Proteolytic activity and electrophoretic profiles of proteases from seminal plasma of teleost. J Fish Biol 63:1008–1019
Kowalski R, Glogowski J, Kucharczyk D et al (2004) Characterization of gelatinolytic activity in seminal plasma of some teleost fish. Aquac Int 12:57–68
Lahnsteiner F, Mansour N, Berger B (2004) Seminal plasma proteins prolong the viability of rainbow trout (Oncorhynchus mykiss) spermatozoa. Theriogenology 62:801–808
Lodemel JB, Maehre HK, Winberg JO et al (2004) Tissue distribution, inhibition and activation of gelatinolytic activities in Atlantic cod (Gadus morhua). Comp Biochem Physiol B Biochem Mol Biol 137:363–371
Lowry O, Rosebrough N, Farr A et al (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Shields RJ (2001) Larviculture of marine finfish in Europe. Aquaculture 20:55–88
Siegel M, Polakoski K (1985) Evaluation of human sperm proscrosin-acrosin system using gelatine-sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Biol Reprod 32:713–720
Uriel J, Berges J (1968) Characterisation of natural inhibitors of trypsin and chymotrypsin by electrophoreses in acrylamide-agarose gels. Nature 218:578–580
Vermeirssen E, Shields RJ, Mazora de quero C et al (2000) Gonadotrophin – releasing hormone agonist raises plasma concentrations of progestogens and enhances milt fluidity in male Atlantic halibut (Hippoglossus hippoglossus). Fish Physiol Biochem 22:77–87
Weltzien F, Taranger G, Karlsen Ø et al (2002) Spermatogenesis and related plasma androgen levels in Atlantic halibut (Hippoglossus hippoglossus L.). Comp Biochem Physiol 132:567–575
Wojtczak M, Ciereszko A (2005) Improvement of electrophoretic detection of antitrypsin activity in fish blood and seminal plasma. Electrophoresis 26:514–516
Wojtczak M, Dietrich GJ, Ciereszko A (2005) Transferrin and antiproteases are major proteins of common carp seminal plasma. Fish Shellfish Immunol 19:387–391
Zhang T, Zhou HM, Liu YX (1997) Expression of plasminogen activator and inhibitor, urokinase receptor and inhibin subunits in thesus monkey tests. Mol Hum Reprod 3:223–231
Acknowledgments
We would like to thank Kiron Viswanath for sampling the seminal plasma and Halina Karol for excellent technical assistance. The study was supported from Forskningsutvalget, Bodø University College.
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Mommens, M., Wojtczak, M., Ciereszko, A. et al. Seminal plasma proteins of Atlantic halibut (Hippoglossus hippoglossus L.). Fish Physiol Biochem 34, 349–355 (2008). https://doi.org/10.1007/s10695-007-9194-x
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DOI: https://doi.org/10.1007/s10695-007-9194-x
Keywords
- Anti-proteolytic activity
- Protein concentration
- Proteolytic activity