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Haemato-biochemical responses and induction of HSP70 to dietary phosphorus in Catla catla (Hamilton) fingerlings

Fish Physiology and Biochemistry volume 34, pages 299–306 (2008)Cite this article

Abstract

A feeding trial of 120 days was conducted to study the effect of graded levels of dietary phosphorus on haematology, serum protein concentrations and HSP70 expression in fingerlings of the Indian major carp, Catla (Catla catla). Eight isonitrogenous and isoenergetic purified diets were formulated to contain graded levels of dietary phosphorus (dP), i.e., T1, 0.1%; T2, 0.3%; T3, 0.5%; T4, 0.7%; T5, 0.9%; T6, 1.1%; T7, 1.3%; or T8, 1.5%. Four hundred and eighty fish (average weight 4.23 ± 0.016 g) were equally distributed into 24 tanks forming eight treatments with three replicates each. The fish were fed daily at the rate of 3.5% body weight in two instalments. At the end of feeding trial fish were sampled to study total RBC and WBC count, haemoglobin, serum lysozyme activity, serum total protein, albumin (A), globulin (G) concentration and HSP70 expression. Total RBC count, haemoglobin concentration and serum lysozyme activity did not vary significantly in response to different dietary phosphorus concentrations. Total WBC count was found to be significantly (P < 0.05) higher in T1 relative to all other treatments. Serum albumin and A/G ratio was found to be significantly lower in fish of T1 and T2 in relation to T7 group (P < 0.05). Serum globulin and total protein levels remained unaffected by variations in dietary phosphorus. HSP70 expression was observed in T1 group (0.1% dP) in gills and brain tissue, but not in liver and muscle tissues. No HSP70 expression was observed in fish of T4 (0.7% dP) and T8 (1.5% dP) treatments. These prima facie results suggest that dietary phosphorus had only minor influence on the haemato-biochemical parameters studied; however dietary phosphorus deficiency caused organ specific induction of HSP70 in catla fingerlings.

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Acknowledgements

The financial assistance received by the first author from the Central Institute of Fisheries Education, Mumbai, for the study is gratefully acknowledged. Authors are thankful to the Director, Central Institute of Fisheries Education, Mumbai, India, for providing all the facilities required for the present work.

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Authors and Affiliations

  1. Fish Biochemistry Laboratory, Central Institute of Fisheries Education, Fisheries University Road, Versova, Mumbai, 400 061, India

    K. Sukumaran, A. K. Pal, N. P. Sahu & R. S. Dalvi

  2. Homi Bhabha Centre for Science Education, Tata Institute of Fundamental Research, Mankhurd, Mumbai, 400 088, India

    D. Debnath

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  1. K. Sukumaran
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  2. A. K. Pal
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  3. N. P. Sahu
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  4. R. S. Dalvi
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Correspondence to A. K. Pal.

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Sukumaran, K., Pal, A.K., Sahu, N.P. et al. Haemato-biochemical responses and induction of HSP70 to dietary phosphorus in Catla catla (Hamilton) fingerlings. Fish Physiol Biochem 34, 299–306 (2008). https://doi.org/10.1007/s10695-007-9188-8

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  • DOI: https://doi.org/10.1007/s10695-007-9188-8

Keywords

  • Phosphorus
  • Catla
  • Haematology
  • Serum protein
  • Lysozyme
  • HSP70

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