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Effects of different artificial photoperiods and temperatures on haematological parameters of rainbow trout (Oncorhynchus mykiss)

Fish Physiology and Biochemistry volume 34, pages 159–167 (2008)Cite this article

Abstract

This study assessed the effects of two artificial photoperiods (LD 24:0 and LD 12:12) and three temperature regimes (9, 11, and 18°C) for 30 days on haematological parameters of trout (Oncorhynchus mykiss) kept in freshwater. Samples were taken at days 7, 14, and 30 during exposure to treatments. A higher mortality (22%) and lower oxygen concentration (<8.0 mg/l) were associated with the combination of photoperiod LD 24:0 and 18°C. The LD 24:0 photoperiod (independently of temperature) increased the haematocrit and the number of erythrocytes at days 7, 14, and 30 (P < 0.01). A temperature of 18°C (independently of photoperiod regimes) diminished the number of total leucocytes, lymphocytes and thrombocytes (P < 0.01). The LD 24:0 photoperiod (also independently of temperature) lowered the number of lymphocytes only after 14 days of experimentation (P < 0.01). Interaction between artificial photoperiod and temperature was only observed at day 14 for polychromatophils (P < 0.01). These results resemble the effects of stress caused by elevated temperatures and the application of continuous light photoperiods, indicating that survival risks may develop in trout farming when this combination is met.

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Acknowledgements

We would like to thank all the agencies, institutions and companies that made this investigation possible: CONICYT (Chile) FONDEF grants D96 I 1090 and D00-T-2032; FONDAP grant: O. & B.M. (fishes subgrant); and the Biotechnology Centre and the Faculty of Natural Sciences at the University of Concepción. Thanks also go to our collaborators Ms. Katherine Alveal, Mr. Alex Muñoz and Mr. George Guala-Knabe. Special thanks are due to Dr. Mark Porter (University of Tasmania, now at Ridley Aqua-Feed Pty Ltd. Queensland, Australia) for critically reviewing this article.

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Authors and Affiliations

  1. Department of Oceanography, Faculty of Natural Sciences and Oceanography, University of Concepción, PO Box 160-C, Concepcion, Chile

    Ariel E. Valenzuela & Alfredo E. Klempau

  2. Department of Clinical Biochemistry and Immunology, Faculty of Pharmacy, University of Concepción, Concepcion, Chile

    Victor M. Silva

Authors
  1. Ariel E. Valenzuela
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  2. Victor M. Silva
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  3. Alfredo E. Klempau
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Correspondence to Ariel E. Valenzuela.

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Valenzuela, A.E., Silva, V.M. & Klempau, A.E. Effects of different artificial photoperiods and temperatures on haematological parameters of rainbow trout (Oncorhynchus mykiss). Fish Physiol Biochem 34, 159–167 (2008). https://doi.org/10.1007/s10695-007-9156-3

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  • DOI: https://doi.org/10.1007/s10695-007-9156-3

Keywords

  • Photoperiod
  • Temperature
  • Stress
  • Blood
  • Oxygen levels
  • Erythrocytes
  • Leucocytes

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