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Bioaccumulation of hexachlorocyclohexane, dichlorodiphenyltrichloroethane, and estradiol-17β in catfish and carp during the pre-monsoon season in India

Fish Physiology and Biochemistry volume 34, pages 25–36 (2008)Cite this article

Abstract

This investigation was performed to monitor hexachlorocyclohexane isomers (HCHs), dichlorodiphenyltrichloroethane (DDT, and its metabolites, refered to as DDTs), plasma levels of estradiol-17β (E2), and the gonadosomatic index (GSI) between sampling sites of unpolluted ponds of Gujartal, Jaunpur (control site) and the polluted rivers Gomti (Jaunpur) and Ganga (Varanasi), which affect the reproductive physiology of some edible catfish and carp during the pre-monsoon season. HCHs and DDTs were measured by gas liquid chromatography (GLC) and hormones by radioimmunoassay (RIA). The results indicated that the level of HCHs and DDTs was very high in both the catfish and the carp captured from the polluted rivers compared with the fish captured from the control site. The GSI and E2 values were lower in both groups of fish when compared to the fish from the control site. The results also indicate that catfish showed greater bioaccumulation of HCHs and DDTs than carp, above the permissible limit, as compared to the fish from the control site. In conclusion, fish from the Gomti and Ganga rivers were highly polluted when compared with fish from the control site, as was evident from high levels of tissue bioaccumulation of HCHs and DDTs and decreased levels of plasma E2, inhibiting the reproductive physiology of these species at the receptor level. The levels exceeded the maximum residue limits (MRL) as recommended by Codex, hence it is suggested that the fish should be avoided for food purposes.

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References

  • Aditya AK, Chattopadhyaya S (2000) Accumulation of methyl parathion in the muscle and gonad of Labeo rohita. J Environ Biol 21(1):55–57

    CAS  Google Scholar 

  • Anonymous (1998) Guidelines for the treatment of animals in behavioral research and teaching. Anim Behav 55:251–257

    Article  Google Scholar 

  • Ahmad S, Ajmal M, Nomani AA (1996) Organochlorines and polycyclic aromatic hydrocarbons in the sediments of Ganga river (India). Bull Environ Contam Toxicol 57:794–802

    Article  PubMed  CAS  Google Scholar 

  • El Beit IOD, Wheelock JV, Cotton DE (1981) Factors affecting soil residues of dieldrin, endosulfan γ-HCH dimethoate and pyrolan. Ecotoxicol Environ Saf 5:135–160

    Google Scholar 

  • Barse AV, Chakrabarti T, Ghosh TK, Pal AK, Jadhao SB (2007) Endocrine disruption and metabolic changes following exposure of Cyprinus carpio to diethyl phthalate. Pest Biochem Physiol 88(1):36–42

    Article  CAS  Google Scholar 

  • Bruning JL, Kintz BL (1977) Computational handbook of statistics, 2nd edn. Freeman, Chicago, IL, USA

    Google Scholar 

  • Burdic GE, Harris EJ, Dean HJ, Walker TM, Skea J, Colby D (1964) The accumulation of DDT in the lake trout and the effect on reproduction. Trans Am Fish Soc 93:127–136

    Article  Google Scholar 

  • Burdic GE, Dean HJ, Skea J, Karcher R, Frisa C (1972) Effect of rate and duration of feeding DDT on reproduction of salmonid fishes reared and held under controlled conditions. NY Fish Game J 19:97–115

    Google Scholar 

  • Codex (1996) Food chemicals Codex. Committee on Food Chemicals Codex 4th edn. Food and Nutrition Board, Institute of Medicine, National Academy of Sciences, National Academy, Washington DC

  • Colborn T, Clemment C (1992) Chemically induced alterations in sexual functional development. The Wildlife Human Connection Princeton Scientific, Princeton, NJ

    Google Scholar 

  • Colborn T, vom Saal FS, Soto AM (1993) Developmental effects of endocrine disrupting chemicals in wildlife and humans. Environ Health Perspect 101:378–384

    Article  PubMed  CAS  Google Scholar 

  • Dale WE, Copeland MF, Hayes WJJr (1965) Chlorinated insecticides in the bodies of peoples in India. Bull World Health Org 33:471–477

    PubMed  CAS  Google Scholar 

  • Edward CA (1970) Persistent pesticides in the environment. Critical reviews in Environment Control, CRC, Cleaveland, Ohio, USA

    Google Scholar 

  • Harding AK, Daston GP, Boyd GR, Lucier GW, Safe SH, Stewart J, Tillitt GE, Van Der Kraak G (2006) Endocrine disrupting chemicals research programme of the US Environmental Protection Agency: summary of the peer-review report. Environ Health Perspect 114:1276–1282

    Article  PubMed  CAS  Google Scholar 

  • Haldar P, Raha P, Bhattacharya P, Choudhury A, Adityachaudhury N (1989) Studies on the residues of DDT and endosulphan occurring in Ganga water. Indian J Environ Health 31:156–161

    Google Scholar 

  • Hellou J, Warren WG, Payne JF (1993) Organochlorines including polychlorinated biphenyls in muscles, liver and ovaries of Cod Gadus morhua. Arch Environ Contam Toxicol 25:497–505

    Article  PubMed  CAS  Google Scholar 

  • Hilmy AM, Badawi HK, Shabana MB (1983a) Organochlorine pesticide residues in 12 freshwater Egyptian fish species with special emphasis on Anguilla vulgaris and Mugil cephalus. Comp Biochem Physiol 76C:163–171

    CAS  Google Scholar 

  • Hilmy AM, Badawi HK, Shabana MB (1983b) Physiological mechanism of toxic action of DDT and endrin in two euryhaline freshwater fishes, Anguilla vulgaris and Mugil cephalus. Comp Biochem Physiol 76C:173–179

    CAS  Google Scholar 

  • Kannan K, Tanabe S, Tatsukawa R (1994) Biodegradation capacity and residue pattern of organochlorines in Ganges river dolphin from India. Toxicol Environ Chem 42:249–261

    Article  CAS  Google Scholar 

  • Kannan K, Tanabe S, Tatsukawa P (1995) Geographical distribution and accumulation features of organochlorine residues in fish in tropical Asia and Oceania. Environ Sci Technol 29:2673–2683

    Article  CAS  Google Scholar 

  • Kaphalia BS, Nigam U, Mehrotra S, Seth TD (1986) Freshwater fish: An indicator of pesticide contamination in the aquatic environment. J Environ Biol 7(1):1–9

    CAS  Google Scholar 

  • Kelce WR, Lambright CR, Gray LE, Robert KP (1994) Vinclozodin and p,p′-DDE alter androgenic gene expression: in vivo confirmation of an androgen receptor mediated mechanism. Toxicol Appl Pharmacol 142:192–200

    Article  Google Scholar 

  • Kelce WR, Stone CR, Laws SC, Gray LE, Kemppainen JA Wilson EM (1995) Persistent DDT metabolite p,p′-DDE is a potent androgen receptor antagonist. Nature 375:581–585

    Article  PubMed  CAS  Google Scholar 

  • Kristensen T, Baatrup E, Bayley M (2006) p,p′-DDE fails to reduce the competitive reproductive fitness in Nigerian male guppies. Ecotoxicol Environ Saf 63(1):148–157

    Article  PubMed  CAS  Google Scholar 

  • Kime DE (1998) Endocrine disruption in fish. Kluwer Academic, Massachusetts, USA

    Google Scholar 

  • Kime DE, Singh PB (1996) In vitro effects of γ-hexachlorocyclohexane on in vitro biosynthesis and metabolism steroids in goldfish, Carassius auratus. Ecotoxicol Environ Saf 34(2):165–173

    Article  PubMed  CAS  Google Scholar 

  • Kulshrestha SK, Krishan G, Jain A (1995) Effect of pesticides on fishes: a review of studies in India. J Natcon 7(2):145–188

    Google Scholar 

  • Kumari A, Sinha RK, Gopal K (2001) Quantitative estimation of DDT residues in some freshwater fishes of river Ganga at Patna, Bihar. Environ Ecol 19(2):396–399

    Google Scholar 

  • Kumari A, Sinha AK, Gopal K, Lata S (2002) Concentration of organochlorines in Ganges river dolphins from Patna, Bihar. J Environ Biol 23(3):279–281

    PubMed  CAS  Google Scholar 

  • Lenardon AM, De Hevia MIM, Fuse JA, De Nochetto CB, Depeteris PJ (1984) Organochlorine, organophosphorus pesticides in the Parana river (Argentina). Sci Total Environ 34:289–297

    Article  CAS  Google Scholar 

  • Letherland JF, Sonstegard RA (1982) Bioaccumulation of organochlorines by yearling coho salmon (Oncorhynchus kisutch Walbaum) fed diets containing Great Lakes coho salmon, and the pathophysiological responses of the recipients. Comp Biochem Physiol 72C:91–99

    Google Scholar 

  • Mills LJ, Gutjahr-Gobell RE, Haebler A, Horoitz DJB, Jayaraman S, Pruell RJ, McKinney RA, Gardner GR, Zaroogian GE (2001) Effect of estrogenic (o,p′-DDT; octylphenol) and anti-androgenic (p,p′-DDE) chemicals on indicators of endocrine status in juvenile male summer flounder (Paralichthys dentatus). Aquat Toxicol 52(2):157–176

    Article  PubMed  CAS  Google Scholar 

  • Nayak, Raha R, Das AK (1995) Organochlorine pesticide residues in middle stream of the Ganges river, India. Bull Environ Contam Toxicol 54:68–75

    Article  PubMed  CAS  Google Scholar 

  • Paasivirta J, Palm H, Paukku R, Akhabuhaya J, Lodenius M (1988) Chlorinated insecticide residues in Tanjanian environment. Chemosphere 17:2055–2062

    Article  CAS  Google Scholar 

  • PFA (1954) The Prevention of Food Adulteration Act (1954) edition 2006 pp 109–119, Universal Law, India

  • Ramesh A, Tanabe S, Taksukawa R, Subramaniun AN, Palanichamy S, Mohan D, Venugopalan VK (1989) Seasonal variations in organochlorine insecticide residues in air from Porto Novo, South India. Environ Pollut 62:213–222

    Article  PubMed  CAS  Google Scholar 

  • Sahagun AM, Tem MT, Garcia JJ, Sierra M, Fernandes N, Fiz MS (1998) Organochlorine pesticide residues in muscles tissues of rainbow trout, Oncorynchus mykiss taken from four fish farms in Leon, Spain. Food Addit Contam 15(5):501–505

    PubMed  CAS  Google Scholar 

  • Senthilkumar K, Kannan K, Sinha RK, Tanabe S, Giesy JP (1999a) Bioaccumulation profiles of polychlorinated biphenyl cogeners and organochlorine pesticides in Ganges river dolphins. Environ Toxicol Chem 18:1511–1520

    Article  CAS  Google Scholar 

  • Senthilkumar K, Watanabe M, Kannan K, Tanabe S, Subramanian AN (1999b) Isomer-specific patterns and toxic assessment of polychlorinated biphenyls in resident and wintering migrant birds and bat collected from south India. Toxicol Environ Chem 71:221–239

    CAS  Google Scholar 

  • Senthilkumar K, Kannan K, Tanabe S, Subramanian AN (2001) Accumulation of persistent organochlorine pesticides and PCBs in sediments, aquatic biota, birds, eggs and bat collected from south India. Environ Sci Poll Res 8:35–47

    CAS  Google Scholar 

  • Sharma A, Verma DK, Sharma M, Singh RP, Khaprde S (1995) Bioconcentration potential of body muscle of seven fresh water fishes to DDT and its isomers: a gas chromatographic investigation. Proc Acad Environ Biol 4:55–58

    CAS  Google Scholar 

  • Singh PB, Singh TP (1990) Seasonal correlative changes between sex steroids and lipid levels in the freshwater female catfish, Heteropneustes fossilis. J Fish Biol 37:793–702

    Article  CAS  Google Scholar 

  • Singh PB, Canario AVM (2004) Reproductive endocrine disruption in the freshwater catfish, Heteropneustes fossilis, in response to the pesticide γ-hexachlorocyclohexane. Ecotoxicol Environ Saf 58(1):77–83

    Article  PubMed  CAS  Google Scholar 

  • Singh PB, Kime DE, Singh TP (1993) Modulatory actions of Mystus gonadotropin on γ-BHC-induced histological changes, cholesterol and sex steroid levels in Heteropneustes fossilis. Ecotoxicol Environ Saf 25:141–153

    Article  PubMed  CAS  Google Scholar 

  • Singh PB, Kime DE, Epler P, Chyb J (1994) Impact of γ-hexachlorocyclohexane exposure on plasma gonadotropin levels and in vitro stimulation of gonadal steroid production by carp hypophyseal hemogonate in Carassius auratus. J Fish Biol 44(2):195–204

    CAS  Google Scholar 

  • Singh PP, Battu RS, Kalra RL (1988) Insecticide residue in wheat grains and straw arising from their storage in premises treated with BHC and DDT under malaria control Programme. Bull Environ Contam Toxicol 44:696–702

    Article  Google Scholar 

  • Singh N, Shukla MM, Chand SK, Sharma VP (1997) Outbreak of falciparum malaria in submerged villages of Narayanganj PHC, district Mandla due to Narmada irrigation Project, Central India (Madhya Pradesh). Curr Sci 73:689–691

    Google Scholar 

  • Singh KP, Malik A, Mohan D, Takroo R (2005) Distribution of persistant organochlorine pesticide residues in Gomti River, India. Bull Environ Contam Toxicol 74:146–154

    Article  PubMed  CAS  Google Scholar 

  • Tanabe S, Kannan K, Tabucanon MS, Siriwong C, Ambe Y, Tatsukawa R (1991) Organochlorine pesticide and polychlorinated biphenyl residues in foodstuffs from, Thailand. Environ Pollut 72:191–203

    Article  PubMed  CAS  Google Scholar 

  • Tomizawa C (1977) Past and present status of residues of pesticides marketed in agriculture commodities in Japan. Japan Pest Inf 30:5–42

    CAS  Google Scholar 

  • Von Westernhagen H, Dethlefsen V, Cameron P, Janssen D (1987) Chlorinated hydrocarbon residues in gonads of marine fish and effects on reproduction. Sarsia 72:419–422

    Google Scholar 

  • Verma DK (1991) Gas liquid Chromatographic analysis of DDT residue in the kidney of Labeo rohita. Acta Hydrochim Hydrobiol 19(3):327–329

    Article  CAS  Google Scholar 

  • Wester PW, Canton JH, Bisschop A (1985) Histopathological study of Poecilia reticulata (guppy) after long term β-hexachlorocyclohexane exposure. Aquat Toxicol 6:271–296

    Article  CAS  Google Scholar 

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Acknowledgements

A grant-in-aid from the Department of Science and Technology, New Delhi-110 016, Government of India to PBS (SR/SO/AS-07/2004 dated 12.7.05) is greatly appreciated. The authors are grateful to the Industrial Toxicology Research Centre (ITRC), Lucknow for GLC, excellent ITRC library facilities, and to Dr S. S. Srivastava for reading of this manuscript.

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  1. Fish Reproductive Toxicology Laboratory, Department of Zoology, Tilak Dhari College, Jaunpur, 222 002, India

    Pratap B. Singh & Vandana Singh

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  1. Pratap B. Singh
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  2. Vandana Singh
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Correspondence to Pratap B. Singh.

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Singh, P.B., Singh, V. Bioaccumulation of hexachlorocyclohexane, dichlorodiphenyltrichloroethane, and estradiol-17β in catfish and carp during the pre-monsoon season in India. Fish Physiol Biochem 34, 25–36 (2008). https://doi.org/10.1007/s10695-007-9142-9

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  • DOI: https://doi.org/10.1007/s10695-007-9142-9

Keywords

  • Catfish
  • Carp
  • Estradiol-17β
  • HCHs
  • DDTs
  • Bioaccumulation
  • Reproduction
  • Steroidogenesis
  • River pollution

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