Abstract
Sox9 is an SRY-related testis-determining gene. It has been identified in different species, thereby testifying to its conserved role in evolution. In this study, we isolated and characterized the guppy sox9b coding sequence and then analyzed its expression pattern in various adult guppy tissues by reverse transcription (RT)-PCR. The guppy sox9b encoded a 480-amino acid (aa) protein containing a SOX-TCF_HMG-box from aa 103 to 174 in the N-terminus region. The predicted molecular weight and the isoelectric point of this protein were 52.7 kDa and 6.61, respectively. The deduced amino acid sequence of guppy Sox9b showed 91.0% similarity to that of Oryzias latipes and 88.3% similarity to that Gasterosteus aculeatus. The detection of Sox9b mRNA in both the testis and ovary suggests that this gene might be involved in the gonad development of guppy.
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Abbreviations
- HMG:
-
High-mobility-group
- RT-PCR:
-
Reverse transcription-polymerase chain reaction
- TA:
-
Trans-activation
References
Baroiller JF, Guigen Y, Forstier A (1999) Endocrine and environmental aspects of sex differentiation in fish. Cell Mol Life Sci 55:910–931
Chiang EF, Pai CI, Wyatt M, Yan YL, Postlethwait J, Chung B (2001) Two sox9 genes on duplicated zebrafish chromosomes, expression of similar transcription activators in distinct sites. Dev Biol 231:149–163
Choudhary B, Ganesh S, Raman R (2000) Evolutionary conservation of the gene Cvsox9 in the lizard, Calotes versicolor, and its expression during gonadal differentiation. Dev Genes Evol 210:250–257
Devlin RH, Nagahama Y (2002) Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences. Aquaculture 208:191–364
Foster JW, Dominguez-Steglich MA, Guioli S, Kwok C, Weller PA, Stevanovic M, Weissenbach J, Mansour S, Young ID, Goodfellow PN, Brook JD, Schafer AJ (1994) Campomelic dysplasia and autosomal sex reversal caused by mutations in an SRY-related gene. Nature 372:525–530
Gubbay J, Collignon J, Koopman P, Capel B, Economou A, Münsterberg A, Vivian N, Goodfellow P, Lovell-Badge R (1990) A gene mapping to the sex-determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes. Nature 346:245–250
Kanai YA, Koopman P (1999) Structural and functional characterization of the mouse Sox9 promoter, implications for campomelic dysplasia. Hum Mol Genet 8:691–696
Kelley LA, Maccallum RM, Sternberg MJE (2000) Enhanced genome annotation using structural profiles in the program 3D-PSSM. J Mol Biol 299:499–520
Khoo G, Lim TM, Chan WK, Phang VPE (1999a) Genetic basis of the variegated tail pattern in the guppy, Poecilia reticulata. Zool Sci 16:431–437
Khoo G, Lim TM, Chan WK, Phang VPE (1999b) Sex linkage of the black caudal-peduncle and red tail genes in the Tuxedo strain of the guppy, Poecilia reticulata. Zool Sci 16:629–638
Khoo G, Lim TM, Chan WK, Phang VPE (1999c). Linkage analysis and mapping of three sex-linked color pattern genes in the guppy, Poecilia reticulata. Zool Sci 16:893–903
Kluver N, Kondo M, Herpin A, Mitani H, Schartl M (2005) Divergent expression patterns of Sox9 duplicates in teleosts indicate a lineage specific subfunctionalization. Dev Genes Evol 215:297–305
Koopman P, Schepers G, Brenner S, Venkatesh B (2004) Origin and diversity of the Sox transcription factor gene family genome-wide analysis in Fugu rubripes. Gene 328:177–186
Matsuda M, Kusama T, Oshiro T, Kurihara Y, Hamaguchi S, Sakaizumi M (1997) Isolation of a sex chromosome-specific DNA sequence in the medaka, Oryzias latipes. Genes Gen Syst 72:263–268
Matsuda M, Nagahama Y, Shinomiya A, Sato T, Matsuda C, Kobayashi T (2002) DMY is a Y-specific DM domain gene required for male development in the medaka fish. Nature 417:559–563
Morais da Silva S, Hacker A, Harley V, Goodfellow P, Swain A, Lovell- Badge R (1996) Sox9 expression during gonadal development implies a conserved role for the gene in testis differentiation in mammals and birds. Nat Genet 14:62–68
Nanda I, Kondo M, Hornung U, Asakawa S, Winkler C (2002) A duplicated copy of DMRT1 in the sex-determining region of the Y chromosome of the medaka, Oryzias latipes. Proc Natl Acad Sci USA 99:11778–11783
Ng LJ, Wheatley S, Muscat GEO, Conway-Campbell J, Bowles J, Wright E, Bell DM, Tam PPL, Cheah KSE, Koopman P (1997) SOX9 binds DNA, activates transcription and co-expresses with type II collagen during chondrogenesis in the mouse. Dev Biol 183:108–121
Preiss S, Argentaro A, Clayton A, John A, Jans DA, Ogata T, Nagai T, Barroso I, Schafer AJ, Harley VR (2001) Compound effects of point mutations causing campomelic dysplasia/autosomal sex reversal upon SOX9 structure, nuclear transport, DNA binding, and transcriptional activation. J Biol Chem 276:27864–27872
Spotila LD, Spotila JR, Hall SE (1998) Sequence and expression analysis of WT1 and Sox9 in the red-eared slider turtle, Trachemys scripta. J Exp Zool 281:417–427
Sudbeck P, Scherer G (1997) Two independent nuclear localization signals are present in the DNA-binding high-mobility group domains of SRY and SOX9. J Biol Chem 272:27848–27852
Sudbeck P, Schmitz ML, Baeuerle PA, Scherer G (1996) Sex reversal by loss of the C-terminal transactivation domain of human SOX9. Nat Genet 13:230–232
Takamatsu N, Kanda H, Ito M, Yamashita A, Yamashita S, Shiba T (1997) Rainbow trout SOX9, cDNA cloning, gene structure and expression. Genet 202:167–170
Takase M, Noguchi S, Nakamura M (2000) Two Sox9 messenger RNA isoforms, isolation of cDNAs and their expression during gonadal development in the frog Rana rugosa. FEBS Lett 466:249–254
Traut W, Winking H (2001) Meiotic chromosomes and stages of sex chromosome evolution in fish: zebrafish, platyfish and guppy. Chromosome Res 9:659–672
Wagner T, Wirth J, Meyer J, Zabel B, Held M, Zimmer J, Pasantes J, Bricarelli FD, Keutel J, Hustert E, Wolf U, Tommerup N, Schempp W, Scherer G (1994) Autosomal sex reversal and campomelic dysplasia are caused by mutations in and around the SRY-related gene SOX9. Cell 79:1111–1120
Watanabe T, Yoshida M, Nakajima M, Taniguchi N (2005) Linkage mapping of AFLP and microsatellite DNA markers with the body color- and sex-determining loci in the guppy (Poecilia reticulata). Zool Sci 22:883–892
Western PS, Harry JL, Graves JAM, Sinclair AH (1999) Temperature dependent sex determination, upregulation of Sox9 expression after commitment to male development. Dev Dynam 214:171–177
Winge O, Ditlevsen E (1947) Colour inheritance and sex determining in lebistes. Heredity 1:65–83
Wright E, Hargrave MR, Christiansen J, Cooper L, Kun J, Evans T, Gangadharan U, Greenfield A, Koopman P (1995) The Sry-related gene Sox9 is expressed during chondrogenesis in mouse embryos. Nat Genet 9:15–20
Yokoi H, Kobayashi T, Tanaka M, Nagahama Y, Wakamatsu Y, Takeda H, Araki K, Morohashi K, Ozato K (2002) Sox9 in a Teleost Fish, Medaka (Oryzias latipes): Evidence for diversified function of Sox9 in gonad differentiation. Mol Reprod Dev 63:5–16
Zhou R, Liu L, Guo Y, Yu H, Cheng H, Huang X, Tiersch TR, Berta P (2003) Similar gene structure of two Sox9a genes and their expression patterns during gonadal differentiation in a teleost fish, rice field eel (Monopterus albus). Mol Reprod Dev 66:211–217
Acknowledgements
The author thanks Dr. Bindhu Paul-Prasanth for critical reading of the manuscript and valuable suggestions. This research was funded by an 863 High Technology Project (2004AA602310) from the Chinese Ministry of Science and Technology.
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Shen, XY., Cui, JZ., Gong, QL. et al. Cloning of the full-length coding sequence and expression analysis of Sox9b in guppy (Poecilia reticulata) . Fish Physiol Biochem 33, 195–202 (2007). https://doi.org/10.1007/s10695-007-9131-z
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DOI: https://doi.org/10.1007/s10695-007-9131-z
Keywords
- mRNA expression
- RT-PCR
- HMG