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Acute effects of copper on superoxide dismutase, catalase and lipid peroxidation in the freshwater teleost fish, Esomus danricus

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Abstract

Static-renewal bioassays [Methods for acute toxicity tests with fish, macro-invertebrates and amphibians: USEPA, ERS, EPA 660/3 75-009 (1975)] were carried out on Esomus danricus exposed to sub-lethal (0.55 mg/l) and lethal (5.5 mg/l) concentrations of copper. The 96-h median lethal concentration (LC50) was 5.5 mg/l. Biochemical stress responses, such as visceral superoxide dismutase (SOD) and catalase (CAT) activities, were measured during this 96-h period. Malondialdehyde, a product of lipid peroxidation, was present at elevated levels in the visceral tissue of copper-exposed fish. Copper was found to be highly toxic to the fish and induced significant declines (p < 0.05–0.001) in all of the biochemical profiles studied, demonstrating a linear and positive correlation with both the concentration and duration of exposure to copper. In E. danricus, CAT appeared to be more sensitive to copper exposure (p < 0.001) than SOD at both lethal and sub-lethal levels. These results indicate that antioxidant responses can be employed as biomarkers of oxidative stress for this species in aquatic environments contaminated with copper.

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Authors and Affiliations

  1. Environmental and Molecular Toxicology Laboratory, Center for Environment, Institute of Science and Technology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad, 500 072, Andhra Pradesh, India

    Sesha Srinivas Vutukuru, Suma Chintada & K. Radha Madhavi

  2. Toxicology Unit, Biology Division, Indian Institute of Chemical Technology, Tarnaka, Hyderabad, 500 007, Andhra Pradesh, India

    J. Venkateswara Rao

  3. Centre for Atmospheric Studies and Weather Modification, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad, 500 072, Andhra Pradesh, India

    Y. Anjaneyulu

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  1. Sesha Srinivas Vutukuru
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  2. Suma Chintada
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  3. K. Radha Madhavi
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  4. J. Venkateswara Rao
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  5. Y. Anjaneyulu
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Correspondence to Sesha Srinivas Vutukuru.

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Vutukuru, S., Chintada, S., Radha Madhavi, K. et al. Acute effects of copper on superoxide dismutase, catalase and lipid peroxidation in the freshwater teleost fish, Esomus danricus . Fish Physiol Biochem 32, 221–229 (2006). https://doi.org/10.1007/s10695-006-9004-x

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  • DOI: https://doi.org/10.1007/s10695-006-9004-x

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