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Studies on digestibility and digestive enzyme activities in Labeo rohita (Hamilton) juveniles: effect of microbial α-amylase supplementation in non-gelatinized or gelatinized corn-based diet at two protein levels

Fish Physiology and Biochemistry volume 32, pages 209–220 (2006)Cite this article

Abstract

A 60-days feeding trial was conducted to delineate the effect of both gelatinized and non-gelatinized corn with or without supplementation with exogenous α-amylase at two dietary protein levels (35% and 28%) on dry matter digestibility, digestive enzymes and tissue glycogen content of Labeo rohita juveniles. Three hundred and sixty juveniles (average weight 10±0.15 g] were randomly distributed into 12 treatment groups with each of two replicates. Twelve semi-purified diets containing either 35% or 28% crude protein were prepared by including gelatinized (G) or non-gelatinized (NG) corn as carbohydrate source with different level of microbial α-amylase (0, 50, 100 and 150 mg kg−1). The dry matter digestibility of G corn fed groups was significantly higher (P < 0.05) than that of the NG corn fed groups. Hepatosomatic index (HSI), liver glycogen and intestinal amylase activity of G starch fed groups were significantly higher (P < 0.05) than those of the NG corn fed groups. However, the reverse trend was found for gastrosomatic index (GSI), muscle glycogen and intestinal protease activity. Addition of 50 mg α-amylase kg−1 feed improved the dry matter digestibility of NG starch fed groups, which was similar to that of the G corn fed groups or NG corn supplemented with 100/150 mg α-amylase kg−1 feed. HSI, liver glycogen and intestinal amylase activity were significantly increased (P < 0.05) at minimum level of α-amylase in the feed (50 mg kg−1) and did not increase due to further inclusion of amylase in the diet. Supplementation with α-amylase at 50 mg kg−1 increased the intestinal amylase activity beyond which no significant changes were observed. Protease activity of liver and intestine was highest (P < 0.05) in higher crude protein (CP) fed groups, but protease activity of the intestine was significantly higher in the α-amylase supplemented groups. Hence, it was concluded that feed with 28% CP containing either G corn without α-amylase or NG corn with 50 mg α-amylase kg−1 may be used as the alternative carbohydrate source for L. rohita juveniles.

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Acknowledgements

The authors are grateful to the Director, Central Institute of Fisheries Education, Mumbai, for providing facilities for carrying out the work. S.␣Kumar is grateful to Central Institute of Fisheries Education for awarding the institutional fellowship.

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Authors and Affiliations

  1. Department of Fish Nutrition and Biochemistry, Central Institute of Fisheries Education, Versova, Mumbai, 400061, India

    S. Kumar, N. P. Sahu, A. K. Pal & D. Choudhury

  2. Department of Fish Pathology and Microbiology, Central Institute of Fisheries Education, Versova, Mumbai, India

    S. C. Mukherjee

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  1. S. Kumar
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  2. N. P. Sahu
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  4. D. Choudhury
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Correspondence to S. Kumar.

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Kumar, S., Sahu, N.P., Pal, A.K. et al. Studies on digestibility and digestive enzyme activities in Labeo rohita (Hamilton) juveniles: effect of microbial α-amylase supplementation in non-gelatinized or gelatinized corn-based diet at two protein levels. Fish Physiol Biochem 32, 209–220 (2006). https://doi.org/10.1007/s10695-006-9002-z

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Keywords

  • Apparent digestibility
  • α-Amylase
  • Carbohydrate
  • Gelatinization
  • Glycogen
  • Labeo rohita
  • Protease

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