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Molecular cloning and sequencing of hemoglobin-β gene of channel catfish, Ictalurus punctatus Rafinesque

Fish Physiology and Biochemistry volume 32, pages 83–92 (2006)Cite this article

Abstract

The hemoglobin-β gene of channel catfish, Ictalurus punctatus, was cloned and sequenced. Total RNA from head kidneys was isolated, reverse transcribed and amplified. The sequence of the channel catfish hemoglobin-β gene consists of 600 nucleotides. Analysis of the nucleotide sequence reveals one open reading frame and 5’- as well as 3’-untranslated regions. The open reading frame of the sequence potentially encodes 148 amino acids with a calculated molecular mass of 16.3 kDa. The pI and charge at pH 7.0 of the deduced hemoglobin-β protein were 7.28 and 0.47, respectively. Overall, 22 amino acid residues were conserved throughout the sequences, including His64 and His93, the sites for heme-binding. Unlike the counterpart of other common cultured fish such as Salmo salar, Oncorhynchus nerka, Oncorhynchus mykiss, Cyprinus carpio and Ctenopharyngodon idella, the hemoglobin-β of channel catfish did not have cysteine. The amino acid sequence of channel catfish hemoglobin-β shows 84% homology with that of Silurus asotus (both are in the order Siluriformes). However, comparison with those of other fish species shows homology ranging from 53 to 68%. Structural analysis by the 3D-PSSM program displays that channel catfish hemoglobin-β has eight α-helices, A–H.

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  1. United States Department of Agriculture, Agricultural Research Service, Aquatic Animal Health Research Unit, 990 Wire Road, P.O. Box 952, Auburn, AL, 36831-0952, USA

    Hung-Yueh Yeh, Craig A. Shoemaker & Phillip H. Klesius

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  1. Hung-Yueh Yeh
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  2. Craig A. Shoemaker
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  3. Phillip H. Klesius
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Correspondence to Hung-Yueh Yeh.

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Yeh, HY., Shoemaker, C.A. & Klesius, P.H. Molecular cloning and sequencing of hemoglobin-β gene of channel catfish, Ictalurus punctatus Rafinesque. Fish Physiol Biochem 32, 83–92 (2006). https://doi.org/10.1007/s10695-006-7658-z

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  • DOI: https://doi.org/10.1007/s10695-006-7658-z

Keywords

  • Channel catfish
  • Ictalurus punctatus
  • Hemoglobin-β
  • Aquaculture

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