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Alterations in enzyme activities in vital organs of triploid female catfish Heteropneustes fossilis (Bloch)

Fish Physiology and Biochemistry volume 32, pages 73–81 (2006)Cite this article

Abstract

Triploid Heteropneustes fossilis (Bloch) showed sterility and higher growth potential than the normal diploid fish. Activities of some metabolic enzymes such as cytosolic NADP-malate dehydrogenase (NADP-MDH), mitochondrial NAD-malate dehydrogenase (NAD-MDH) and glutamate pyruvate transaminase (GPT) were evaluated in liver, brain and kidney along with glucose-6-phosphate dehydrogenase (G-6-P D) in ovary of female triploid catfish. Activities of these enzymes showed distinct seasonal periodicity, mostly with highest activities in prespawning and spawning periods, in both diploid and triploid catfish but differed in magnitude. In triploid liver, GPT showed higher activity than the diploid counterpart in prespawning and spawning periods. On the contrary, mitochondrial NAD-MDH and cytosolic NADP-MDH in this organ showed a consistent lower activity than the diploid in all stages or in some stages of reproductive cycle respectively. Interestingly, none of the enzymes in brain and kidney of triploid female catfish showed significant changes in comparison to the diploid counterpart. The triploid ovary maintained a significantly lower level of G-6-P D activity throughout the resting, preparatory and pre-spawning periods compared to the diploid ovary. Lower level of malic enzymes (NAD-MDH and NADP-MDH) in liver and G-6-P D in ovary are in close synchrony with lower level of estradiol-17β in plasma of female triploids as found in earlier study.

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Authors and Affiliations

  1. Department of Animal Physiology, Bose Institute, P-1/12 CIT, Scheme VII M, Calcutta, 700054, India

    Angshuman Biswas, Mitali Pramanik, Samita Kundu, Sumedha Roy, Jhuma De & Arun Kumar Ray

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  1. Angshuman Biswas
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  2. Mitali Pramanik
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  3. Samita Kundu
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  4. Sumedha Roy
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  5. Jhuma De
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  6. Arun Kumar Ray
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Correspondence to Arun Kumar Ray.

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Biswas, A., Pramanik, M., Kundu, S. et al. Alterations in enzyme activities in vital organs of triploid female catfish Heteropneustes fossilis (Bloch). Fish Physiol Biochem 32, 73–81 (2006). https://doi.org/10.1007/s10695-006-7657-0

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  • DOI: https://doi.org/10.1007/s10695-006-7657-0

Keywords

  • Brain
  • Cytosolic NADP-malate dehydrogenase
  • Fish
  • Glucose 6-phosphate dehydrogenase
  • Glutamate pyruvate transaminase
  • Kidney
  • Liver
  • Mitochondrial NAD-malate dehydrogenase
  • Ovary

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