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Purification of vitellogenin from the air breathing catfish, Clarias gariepinus

Fish Physiology and Biochemistry volume 31, pages 235–239 (2005)Cite this article

Abstract

Vitellogenin (Vtg) is a female specific glycophospholipoprotein which can be induced both in male and female with estradiol and xeno-estrogens. The basic theme behind the purification of vitellogenin from the fish is to understand the evolutionary relationship and for the purification and characterization of the Vtg receptor. The male catfish, Clarias gariepinus was administrated with estradiol over a period of time for the synthesis of Vtg and the serum was collected. The Vtg was purified from the serum using a two step chromatographic technique. The serum was passed on to DEAE-ion exchange column and the protein was eluted using a salt gradient. The fractions containing the Vtg were pooled and passed onto a gel permeation chromatography column and the pure protein was obtained. The molecular weight is around 200 kDa on the SDS-PAGE and around 520 kDa on the native gel electrophoresis.

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Authors and Affiliations

  1. Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, 500 046, India

    D. Manohar, G. Damodar Rao, G. Sreenivasulu, B. Senthilkumaran & Aparna Dutta Gupta

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  1. D. Manohar
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  2. G. Damodar Rao
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  3. G. Sreenivasulu
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  4. B. Senthilkumaran
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  5. Aparna Dutta Gupta
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Correspondence to Aparna Dutta Gupta.

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Manohar, D., Rao, G.D., Sreenivasulu, G. et al. Purification of vitellogenin from the air breathing catfish, Clarias gariepinus . Fish Physiol Biochem 31, 235–239 (2005). https://doi.org/10.1007/s10695-006-0030-5

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  • DOI: https://doi.org/10.1007/s10695-006-0030-5

Keywords

  • Clarias gariepinus
  • Vitellogenin
  • Estradiol-17β

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