Abstract
Growth hormone (GH) has recently been identified as co-gonadotropin regulating fish reproduction, hitherto, no effort has been made to see its effect on oocyte maturation in fishes, though some reports demonstrate the role of insulin like growth factor-I (IGF-I) in oocyte maturation in teleosts. Hence, effect of GH on oocyte maturation in post-vitellogenic H. fossilis has been worked out in the present study. Post-vitellogenic follicles in the ovarian tissue were challenged in vitro with H. fossilis pituitary homogenate (fPH), Clarias batrachus GH and GtH, barramundi IGF-I (IGF-I), 17α, 20β-dihydroxy-4-pregnen-3-one (DHP) and testosterone alone, or in combination with IGF-I for 18 h at 26±1°C. Incubation of ovarian tissue with GH in the presence of actinomycin d or cycloheximide or barramundi IGF-I antiserum was also made separately. In general, oocyte maturation was induced by fPH, barramundi IGF-I, GtH, GH and DHP, which was augmented further by addition of barramundi IGF-I. Testosterone had no effect on GVBD. Actinomycin d, cycloheximide and anti barramundi IGF-I abolished the GH induced oocyte maturation. Present study suggests for the first time that GH has a role in egg maturation in fish.
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Sarang, M.K., Lal, B. Effect of piscine GH/IGF-I on final oocyte maturation in vitro in Heteropneustes fossilis . Fish Physiol Biochem 31, 231–233 (2005). https://doi.org/10.1007/s10695-006-0029-y
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DOI: https://doi.org/10.1007/s10695-006-0029-y
Keywords
- Actinomycin d
- Barramundi IGF-I
- Cycloheximide
- Fish gonadotropin
- Growth hormone
- GVBD
- Ovarian follicles