Abstract
Partial cDNAs encoding carbonyl reductase like 20β-hydroxysteroid dehydrogenase (20β-HSD) and P450 17α-hydroxylase/c17–20 lyase (CYP17) were isolated from the ovary of snake head murrel and they exhibited high sequence identity to the Nile tilapia and rainbow trout, respectively. A low transcript level of both 20β-HSD and CYP17 were detected in pre-vitellogenic follicles, while the transcript level was high in full-grown immature follicles. In hCG-induced in vitro oocyte maturation, we found a significant increase in 20β-HSD transcript level after 2 h. The CYP17 transcripts also showed a considerable increase following hCG-induction compared to saline-treated controls. On the other hand, Western blot analysis demonstrated no significant change in the CYP17 protein level during hCG-induced in vitro oocyte maturation. Taken together, we suggest that in addition to 20β-HSD, the CYP17 might have a role in the shift in steroidogenesis during meiotic maturation of snake head murrel.
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Sreenivasulu, G., Swapna, I., Rasheeda, M.K. et al. Expression of 20β-hydroxysteroid dehydrogenase and P450 17α-hydroxylase/c17–20 lyase during hCG-induced in vitro oocyte maturation in snake head murrel Channa striatus . Fish Physiol Biochem 31, 227–230 (2005). https://doi.org/10.1007/s10695-006-0028-z
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DOI: https://doi.org/10.1007/s10695-006-0028-z
Keywords
- 20β-HSD
- Channa striatus
- CYP17
- Oocyte maturation
- Steroidogenic shift