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Seabream GnRH: partial cDNA cloning, localization and stage-dependent expression in the ovary of snake head murrel, Channa striatus

Fish Physiology and Biochemistry volume 31, pages 157–161 (2005)Cite this article

Abstract

Vertebrate reproduction is under the neuroendocrine control of the hypothalamic decapeptide GnRH which synchronizes various reproductive events and influences other reproduction related aspects like spawning behavior and pheromonal action in fish. Multiple forms of GnRH peptides have been reported across diverse vertebrate and invertebrate classes. Here we report the partial seabream GnRH (sbGnRH) cDNA sequence cloned from the brain of Channa striatus (snake head murrel) a fresh water perciform with immense economic and medicinal value across Asiatic countries. sbGnRH mRNA was found in brain, gill and ovary of mature murrel with possible implications to the effect of GnRH on pheromonal phenomena and on reinitiation of oocyte meiosis. In keeping with the earlier reported role of GnRH in initiation of oocyte meiosis we here present evidence from RT-PCR, ICC demonstrating an increase in the level of sbGnRH mRNA in ovary from pre-vitellogenic to post-vitellogenic follicles.

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References

  • Andreu-Vieyra CV, Buret AG, Habibi HR (2005) Gonadotropin-releasing hormone induction of apoptosis in the testes of goldfish (Carassius auratus). Endocrinology 146:1588–1596

    Article  CAS  PubMed  Google Scholar 

  • Cameron CB, Mackie GO, Powell JFF, Lescheid DW, Sherwood NM (1999) Gonadotropin-releasing hormone in mulberry cells of Saccoglossus and Ptychodera (Hemichordata: Enteropneusta). Gen Comp Endocrinol 114:2–10

    Article  CAS  PubMed  Google Scholar 

  • Kwon JY, McAndrew BJ, Penman DJ (2001) Cloning of brain aromatase gene and expression of brain and ovarian aromatase genes during sexual differentiation in genetic male and female Nile tilapia Oreochromis niloticus. Mol Reprod Dev 59:359–370

    Article  CAS  PubMed  Google Scholar 

  • Marchant TA, Chang JP, Nahorniak CS, Peter RE (1989) Evidence that gonadotropin-releasing hormone also functions as a growth hormone-releasing factor in the goldfish. Endocrinology 124:2509–2518

    Article  CAS  PubMed  Google Scholar 

  • Muske LE (1993) Evolution of gonadotropin-releasing hormone (GnRH) neuronal systems. Brain Behav Evol 42:215–230

    Article  CAS  PubMed  Google Scholar 

  • Pati D, Habibi HR (2002) Involvement of protein kinase C and arachidonic acid pathways in the gonadotropin-releasing hormone regulation of oocyte meiosis and follicular steroidogenesis in the goldfish ovary. Biol Reprod 66:813–822

    Article  CAS  PubMed  Google Scholar 

  • Senthilkumaran B, Okuzawa K, Gen K, Ookura T, Kagawa H (1999) Distribution and seasonal variation of three native GnRHs in the brain and pituitary of perciform fish. J Neuroendocrinol 11:181–186

    Article  CAS  PubMed  Google Scholar 

  • Sherwood NM, von Schalburg K, Lescheid DW (1997) Origin and evolution of GnRH in vertebrates and invertebrates. In: Parhar IS, Sakuma Y (eds) GnRH neurons: gene to behavior. Brain Shuppan, Tokyo, pp 3–25

    Google Scholar 

  • Shimizu A, Tanaka H, Kagawa H (2003) Immunocytochemical applications of specific antisera raised against synthetic fragment peptides of mummichog GtH subunits: examining seasonal variations of gonadotrophs (FSH cells and LH cells) in the mummichog and applications to other acanthopterygian fishes. Gen Comp Endocrinol 132:35–45

    Article  CAS  PubMed  Google Scholar 

  • Weber GM, Powell JFF, Park M, Fischer WH, Craig AG, Rivier JE, Nanakorn U, Parhar IS, Ngamvongchon S, Grau EG, Sherwood NM (1997) Evidence that gonadotropin-releasing hormone (GnRH) functions as a prolactin-releasing factor in a teleost fish (Oreochromis mossambicus) and primary structures for three native GnRH molecules. J Endocrinol 155:121–132

    Article  CAS  PubMed  Google Scholar 

  • Wiechmann CR, Dionne VE (2000) Neuromodulatory effects of gonadotropin releasing hormone on olfactory receptor neurons. J Neurosci 20:3947–3955

    Google Scholar 

  • Wong TT, Zohar Y (2004) Novel expression of gonadotropin subunit genes in oocytes of the gilthead seabream (Sparus aurata). Endocrinology 145:5210–5220

    Article  CAS  PubMed  Google Scholar 

  • Yamamoto N, Oka Y, Kawashima S (1997) Lesions of gonadotropin-releasing hormone-immunoreactive terminal nerve cells: effects on the reproductive behavior of male dwarf gouramis. Neuroendocrinology 65:403–412

    Article  CAS  PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad, 500 046, India

    I. Swapna, G. Sreenivasulu, M. K. Rasheeda & B. Senthilkumaran

  2. Center for Cellular and Molecular Biology, Hyderabad, India

    K. Thangaraj

  3. National Institute of Ocean Technology, Pallikaranai, Chennai, 601 230, India

    R. Kirubagaran

  4. SEAFDEC/AQD, Tigbauan, 5021, Iloilo, Phillipines

    K. Okuzawa

  5. Department of Biological Production and Environmental Science, Faculty of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan

    H. Kagawa

Authors
  1. I. Swapna
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  2. G. Sreenivasulu
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  3. M. K. Rasheeda
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  4. K. Thangaraj
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  5. R. Kirubagaran
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  6. K. Okuzawa
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  7. H. Kagawa
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  8. B. Senthilkumaran
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Corresponding author

Correspondence to B. Senthilkumaran.

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Swapna, I., Sreenivasulu, G., Rasheeda, M.K. et al. Seabream GnRH: partial cDNA cloning, localization and stage-dependent expression in the ovary of snake head murrel, Channa striatus . Fish Physiol Biochem 31, 157–161 (2005). https://doi.org/10.1007/s10695-006-0019-0

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  • DOI: https://doi.org/10.1007/s10695-006-0019-0

Keywords

  • Channa striatus
  • Reinitiation of oocyte meiosis
  • sbGnRH
  • Vitellogenic follicles

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