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Promoter analysis of two aromatase genes in the serial-sex changing gobiid fish, Trimma okinawae

Fish Physiology and Biochemistry volume 31, pages 123–127 (2005)Cite this article

Abstract

Despite numerous endocrine studies on sex change in teleost, no general mechanism that mediates sex change has emerged. The gobiid fish, Trimma okinawae, can change sex in both directions repeatedly. This phenomenon of sex change in goby assigns it as an excellent animal model to elucidate the understanding mechanisms of sex change. In hermaphrodite fishes, estrogen plays a particularly important role in natural and experimentally induced sex change. To investigate the role of estrogen in the serial-sex changing fish T. okinawae, we cloned and analyzed the 5′-flanking regions of P450arom genes from goby genome DNA. Both regions have consensus sequences of TATA, CRE and ERE. Ad4 binding site was restricted in the region of P450aromA. These findings indicate that different regulators control the expression of the two P450arom genes.

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Authors and Affiliations

  1. Sesoko Station, Tropical Biosphere Research Center, University of the Ryukus, 3422 Sesoko, Motobu-cho, Okinawa, 905-0227, Japan

    Yasuhisa Kobayashi & Masaru Nakamura

  2. CREST & SORST, Japan Science and Technology Corporation, Saitama Kawauchi, 332-0227, Japan

    Yasuhisa Kobayashi, Yoshitaka Nagahama & Masaru Nakamura

  3. Natural History Museum and Institute, 955-2, Aoba-cho, Chuo-ku, Chiba, 260-8682, Japan

    Tomoki Sunobe

  4. National Research Institute of Aquaculture, Tamaki, Mie, 516-0423, Japan

    Tohru Kobayashi

  5. Laboratory of Reproduction, National Institute for Basic Biology, Okazaki, 444-8585, Japan

    Yoshitaka Nagahama

Authors
  1. Yasuhisa Kobayashi
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  2. Tomoki Sunobe
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  3. Tohru Kobayashi
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  4. Yoshitaka Nagahama
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  5. Masaru Nakamura
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Corresponding author

Correspondence to Yasuhisa Kobayashi.

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Kobayashi, Y., Sunobe, T., Kobayashi, T. et al. Promoter analysis of two aromatase genes in the serial-sex changing gobiid fish, Trimma okinawae . Fish Physiol Biochem 31, 123–127 (2005). https://doi.org/10.1007/s10695-006-0013-6

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  • DOI: https://doi.org/10.1007/s10695-006-0013-6

Keywords

  • P450 aromatase
  • Sex change
  • Gobiid fish
  • Trimma okinawae
  • Promoter

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