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Starvation Induced Alterations in Hepatic Lysine Metabolism in Different Families of Rainbow Trout (Oncorhynchus  mykiss)

Fish Physiology and Biochemistry volume 31, pages 33–44 (2005)Cite this article

Abstract

Lysine is the second limiting amino acid in fish meal based diets, second only to methionine. However, little is known about lysine metabolism in rainbow trout (RBT). Therefore, lysine catabolism by the lysine α-ketoglutarate reductase (LKR) pathway was studied. Additionally, since genetically improved strains could influence fish production, these studies were performed in 4 distinct families of RBT. Two full-sibling families, differing in feed efficiency, were selected from each of 2 strains (A and B) of RBT. Eight fish from each of the 4 families were allotted to individual tanks. Fish were fed until satiation for 5 weeks when four fish within each family were randomly selected for 2 weeks of starvation. After the starvation period, all fish were harvested. Hepatic in-vitro LKR activity and lysine oxidation were measured as was LKR mRNA. No effect of family within strain on LKR activity or lysine oxidation was detected. Strain A exhibited a 55% reduction (p < 0.01) in LKR transcripts compared to strain B pooled across both feeding levels. Within each family, LKR mRNA was decreased (p < 0.01) in starved vs. fed fish. On average, there was a 68% decrease in LKR transcripts for starved fish. LKR activity averaged 104 ± 33 and 150 ± 31 nmol/min*gm liver (p > 0.1) in fed and starved fish, respectively. Lysine oxidation averaged 1.2 ± 0.5 and 2.2 ± 0.4 nmol/min*gm liver (p > 0.1) in fed and starved fish, respectively. LKR transcripts were positively correlated to weight gain (p < 0.01). These data are consistent with multiple modes of LKR regulation in fish.

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Abbreviations

FCR:

Feed Conversion Ratio

LKR:

Lysine α-ketoglutarate reductase

LOX:

Lysine Oxidation

RBT:

Rainbow Trout

SACDH:

Saccharopine Dehydrogenase

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Authors and Affiliations

  1. Division of Animal and Veterinary Sciences, West Virginia University, P.O. Box 6108, Morgantown, WV, USA, 26506

    Angela D. Higgins, Juanita Engles, Matthew E. Wilson & Kenneth P. Blemings

  2. US Department of Agriculture, Agriculture Research Service, National Center for Cool and Cold Water Aquaculture, 11876 Leetown Rd., Kearneysville, WV, USA, 25430

    Jeffrey T. Silverstein & Caird E. Rexroad III

  3. Program in Genetics and Developmental Biology, West Virginia University, USA

    Angela D. Higgins & Kenneth P. Blemings

Authors
  1. Angela D. Higgins
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  2. Jeffrey T. Silverstein
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  3. Juanita Engles
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  4. Matthew E. Wilson
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  5. Caird E. Rexroad III
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  6. Kenneth P. Blemings
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Correspondence to Kenneth P. Blemings.

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Higgins, A.D., Silverstein, J.T., Engles, J. et al. Starvation Induced Alterations in Hepatic Lysine Metabolism in Different Families of Rainbow Trout (Oncorhynchus  mykiss). Fish Physiol Biochem 31, 33–44 (2005). https://doi.org/10.1007/s10695-005-4587-1

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  • DOI: https://doi.org/10.1007/s10695-005-4587-1

Keywords

  • feed efficiency
  • lysine
  • lysine α-ketoglutarate reductase
  • rainbow trout

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