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Protein and Energy Metabolism of European Seabass (Dicentrarchus labrax) Juveniles and Estimation of Maintenance Requirements

Fish Physiology and Biochemistry volume 31, pages 23–31 (2005)Cite this article

Abstract

The present work aimed to study metabolic utilization of energy and protein by juvenile European seabass reared at 25 °C and to estimate maintenance requirements, based on indirect calorimetry and nitrogen excretion measurements. Duplicate groups of fish (IBW = 22 g) were fed a practical diet, for 28 days, at ration levels of 0, 0.25, 0.5, 1 and 1.5% of body weight. At the end of this period, oxygen consumption and ammonia nitrogen excretion were measured in fed and unfed fish. Weight gain linearly increased with ration level. Feed efficiency significantly increased with nitrogen intake up to 0.68 g N/kg/day. Nitrogen retention (% N intake) was positive at all feeding levels, being maximum at 0.5% BW and then decreased with increasing ration levels. There was negative energy balance at the lowest ration level and significantly increased at higher ration levels. Daily oxygen consumption (g/kg body mass) and ammonia excretion (mg N–NH4/kg body mass) significantly increased with ration level. Heat production and heat increment of feeding (kJ/kg/day) remained low at low ration levels and significantly increased as ration level increased from 0.5 to 1.5% BW. Below maintenance, protein energy represented 34% of total energy expenditure and significantly increased to 49–56% above maintenance. Based on indirect calorimetry and ammonia excretion data, maintenance energy and protein requirements were estimated to be 56.8 kJ DE/kg/day and 1.15 g DP/kg/day, respectively. The efficiencies of energy and protein utilization were estimated to be 0.83 and 0.66, respectively.

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Authors and Affiliations

  1. CIIMAR-Centro Interdisciplinar de Investigação, Marinha e Ambiental Rua dos Bragas, 289, 4050-123, Porto, Portugal

    H. Peres & A. Oliva-Teles

  2. Departamento de Zoologia e Antropologia faculdade de Ciências, Universidade do Porto, Praça Gomes Teixeira, 4099-002, Porto, Portugal

    A. Oliva-Teles

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  1. H. Peres
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  2. A. Oliva-Teles
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Correspondence to A. Oliva-Teles.

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Peres, H., Oliva-Teles, A. Protein and Energy Metabolism of European Seabass (Dicentrarchus labrax) Juveniles and Estimation of Maintenance Requirements. Fish Physiol Biochem 31, 23–31 (2005). https://doi.org/10.1007/s10695-005-4586-2

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  • DOI: https://doi.org/10.1007/s10695-005-4586-2

Keywords

  • energy utilization
  • maintenance requirements
  • nitrogen excretion
  • oxygen consumption
  • protein utilization

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