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Pyridoxine deficiency of grouper, Epinephelus coioides: physiological and biochemical alteration

Fish Physiology and Biochemistry volume 31, pages 331–337 (2005)Cite this article

Abstract

Pyridoxine is essential for animals. The purpose of this study was to investigate the pyridoxine deficiency symptoms and the fit dose of pyridoxine to keep normal physiological function in grouper, Epinephelus coioides. Graded levels of pyridoxine (0, 1, 2, 4, 8, 16, 32 mg pyridoxine kg−1 diet) were fed to grouper juveniles (14.83±0.31 g) for 8 weeks, and then some physiological and biochemical parameters were measured. Pyridoxine deficiency symptoms such as anorexia, poor survival, convulsion, helically swimming and hyperirritability were observed in fish fed the pyridoxine-deficient diet, and these fish also showed low whole blood haemoglobin level, liver glutamic-pyruvic transaminase (GPT) and superoxide dismutase (SOD) activities. Slight symptoms of pyridoxine deficiency also appeared among fish fed the diet with very low dose (1 mg pyridoxine kg−1 diet) of pyridoxine in 6 weeks. Broken-line regression analysis showed that the optimal pyridoxine levels for these fish to keep normal survival, whole blood haemoglobin level, liver GPT and SOD activities were approximately 1.75, 1.87, 2.22 and 2.05 mg kg−1, respectively.

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  1. Institute of Economic Aquatic Animals, School of Life Sciences, Sun Yat-sen University, 135 Xingang West Street, Haizhu District, Guangzhou, 510275, Guangdong Province, The People’s Republic of China

    J. W. Huang, L. X. Tian, Z. Y. Du, H. J. Yang & Y. J. Liu

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  1. J. W. Huang
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  2. L. X. Tian
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  3. Z. Y. Du
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  4. H. J. Yang
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Correspondence to Y. J. Liu.

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Huang, J.W., Tian, L.X., Du, Z.Y. et al. Pyridoxine deficiency of grouper, Epinephelus coioides: physiological and biochemical alteration. Fish Physiol Biochem 31, 331–337 (2005). https://doi.org/10.1007/s10695-005-2832-2

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  • DOI: https://doi.org/10.1007/s10695-005-2832-2

Keywords

  • Epinephelus coioides
  • glutamic-pyruvic transaminase (GPT)
  • grouper
  • haemoglobin
  • pyridoxine deficiency
  • superoxide dismutase (SOD)

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