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Are Primary Cultures of Trout (Oncorhynchus mykiss) Ventricular Cardiomyocytes Metabolically Viable?

Fish Physiology and Biochemistry volume 30, pages 109–117 (2004)Cite this article

Abstract

The objectives of this study were to establish a protocol for the isolation of metabolically viable ventricular cardiomyocytes from the rainbow trout and to determine which measures may best reflect viability 24 h after isolation. Cardiomyocytes were isolated by enzymatic digestion and maintained in cell suspension. Viability was assessed using Trypan blue dye exclusion, ATP content, oxygen consumption and lactate dehydrogenase (LDH) leakage into the medium. Viability, assessed by these measures did not significantly change over the time period of this study. ATP content did correlate significantly with oxygen consumption but not with Trypan blue exclusion. We conclude that primary cultured cardiomyocytes remain metabolically viable for at least 24 h after isolation. Also, it appears that ATP content and oxygen consumption most adequately reflect metabolic cell viability. To be confident with a culture, however, a combination of viability measures is necessary when isolating cardiomyocytes from fish.

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Authors and Affiliations

  1. Department of Biology, Mount Allison University, E4L1G7, Sackville, New Brunswick, Canada

    J.P Goldstein, A. Scott & S. Currie

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  1. J.P Goldstein
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  2. A. Scott
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  3. S. Currie
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Correspondence to S. Currie.

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Goldstein, J., Scott, A. & Currie, S. Are Primary Cultures of Trout (Oncorhynchus mykiss) Ventricular Cardiomyocytes Metabolically Viable?. Fish Physiol Biochem 30, 109–117 (2004). https://doi.org/10.1007/s10695-005-2492-2

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  • DOI: https://doi.org/10.1007/s10695-005-2492-2

Keywords

  • ATP content
  • cardiomyocytes
  • cell viability
  • LDH leakage
  • metabolism
  • oxygen consumption
  • rainbow trout
  • Trypan blue exclusion

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