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Rat Ghrelin Stimulates GH Release and GH mRNA Expressionin the Pituitary of Orange-spotted Grouper, Epinephelus Coioides

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Abstract

Ghrelin was recently demonstrated as an endogenous ligand of the growth hormone (GH) secretagogue receptor (GHS-R), which could promote the release of GH in mammal significantly. The present study conducted to determine whether ghrelin stimulate the release and synthesis of GH in orange-spotted grouper (Epinephelus coioides). Rat ghrelin was incubated with the pituitary fragments of grouper in static culture system. The culture medium was collected at 1, 6, 12, 18 and 24 h after incubation to detect the contents of GH by homologous radioimmunoassay. The level of GH mRNA in the pituitary fragments was measured by a sensitive chemiluminescent ribonuclease protection assay. The results showed that rat ghrelin not only stimulated the release of GH but also augmented the GH mRNA level in grouper. It suggested that the ghrelin-like peptide and the GHS-R involved in the regulation of GH synthesis and release in grouper. The present study would provide a better understanding of the regulatory mechanism of GH release in marine fish.

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Abbreviations

cDNA:

complementary deoxyribonucleic acid

DA:

dopamine

Dig:

digoxigenin

GH:

growth hormone

GHRH:

growth hormone-releasing hormone

GHS-R:

growth hormone secretagogue receptor

GHS:

growth hormone secretagogue

GnRH:

gonadotropin-releasing hormone

Hepes:

N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid

Pipes:

1,4-piperazinebis-ethanesulfoni acid

Pit-1:

pituitary-specific transcription factor

RACE:

rapid amplification of the cDNA ends

RIA:

radioimmunoassay

RPA:

ribonuclease protection assay

SDS:

sodium dodecylsulfate

SS:

somatostatin

SSC:

sodium chloride-sodium citrate

Tris:

tri-hydroxymethyl-aminomethane.

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Authors and Affiliations

  1. Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Zhongshan University, 510275, Guangzhou, China

    Xueqin Ran, Wensheng Li & Haoran Lin

  2. School of Animal Science, Guizhou University, 550025, Guiyang, China

    Xueqin Ran

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  1. Xueqin Ran
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  2. Wensheng Li
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Correspondence to Haoran Lin.

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Ran, X., Li, W. & Lin, H. Rat Ghrelin Stimulates GH Release and GH mRNA Expressionin the Pituitary of Orange-spotted Grouper, Epinephelus Coioides. Fish Physiol Biochem 30, 95–102 (2004). https://doi.org/10.1007/s10695-005-1768-x

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