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Molecular cloning and expression of insulin-like growth factor-I (IGF-I) in Eurasian perch (Perca fluviatilis): lack of responsiveness to growth hormone treatment

Fish Physiology and Biochemistry volume 30, pages 67–76 (2004)Cite this article

Abstract

The cloning of Eurasian perch (Perca fluviatilis) insulin-like growth factor-I (IGF-I) liver cDNA resulted in the identification of two transcripts of 794 and 713 nucleotides. Whereas the long IGF-Ib isoform consisted of 186 residues, the short IGF-Ia isoform was lacking 27 residues of the E domain due to alternative exon splicing. The long isoform was more abundant than the short isoform in liver, while the expression of both isoforms was rather low in non-hepatic tissues. In order to elucidate the specific endocrine regulation of growth in perch, effects of growth hormone (GH) treatment on weight gain and hepatic IGF-I expression were investigated in juvenile Eurasian perch. Fish injected with Southern black bream (Acanthopagrus butcheri, Perciformes) GH (0.5 μg/g body weight) grew significantly faster than the saline injected control group, whereas treatment with bovine GH (1.0 μg/g body weight) gave no growth enhancement. No effects of the GH treatments on hepatic IGF-I mRNA expression were shown by Northern blot analysis. Thus, the impact of GH-IGF-I axis on growth regulation in Eurasian perch remains unclear, but might be affected by IGF-binding proteins (IGFBPs).

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Acknowledgements

We tank Harald Støkken, Sigurd Øxnevad and Roar Elvegård for good assistance at the fish lab. We also acknowledge NIDDK’s National Hormone & Peptide Program and Dr. A.F. Parlow for the shipment of purified bovine GH. This study was funded by the Norwegian Research Council (NFR).

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Authors and Affiliations

  1. Department of Mathematical Sciences and Technology,, Norwegian University of Life Sciences, P.O.Box 5003-1432 Ã…s, Norway

    Sissel Jentoft

  2. Department of Chemistry, Biotechnology and Food Science,, Norwegian University of Life Sciences, P.O.Box 5003-1432 Ã…s, Norway

    Are H. Aastveit

  3. Institute of Aquaculture Research, P.O.Box 5010-1432 Ã…s, Norway

    Øivind Andersen

  4. Aquaculture Protein Centre, P.O.Box 5003-1432 Ã…s, Norway

    Øivind Andersen

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  1. Sissel Jentoft
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  2. Are H. Aastveit
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  3. Øivind Andersen
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Correspondence to Sissel Jentoft.

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Jentoft, S., Aastveit, A.H. & Andersen, Ø. Molecular cloning and expression of insulin-like growth factor-I (IGF-I) in Eurasian perch (Perca fluviatilis): lack of responsiveness to growth hormone treatment. Fish Physiol Biochem 30, 67–76 (2004). https://doi.org/10.1007/s10695-005-0267-4

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Keywords

  • alternative splicing
  • Eurasian perch
  • growth
  • IGF-I cDNA
  • pro-IGF-I

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