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Temporal profile of the outer- and inner-ring iodothyronine deiodinase pathways in the liver and skin in developing rainbow trout Oncorhynchus mykiss

Fish Physiology and Biochemistry volume 29, pages 297–303 (2003)Cite this article

Abstract

We measured the activities of iodothyronine-activating (D1 and D2) and -inactivating (D3) deiodinases, as well as the circulating levels of their corresponding substrates and/or products, T4, T3, and rT3, in the developing rainbow trout. The study was conducted through 20 consecutive months and included fry, fingerlings, and mature animals from 14 to 88 weeks post-hatching (ph). Initially, blood concentration of active iodothyronines was low and tissue D3 activity was greater than the activity of D1 and D2. As fry developed into fingerlings, the activity of liver D2 increased, while that of skin D3 declined. The time course of these changes was directly (T3) or inversely (rT3 and D3) correlated to an increase in age, length, and body weight. The initial increment in both circulating T3 and hepatic D2 activity during the first spring (March-April; weeks 46–50 ph) coincided with the onset of gonadal differentiation, whereas the highest values of T3 and hepatic D1 and D2 (September–October; weeks 70–74 ph) occurred at the onset of sexual maturity. These results suggest that a hepatic supply of active T3 is temporally associated with gametogenesis.

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Abbreviations

ANS:

8-anilin-1-naphthalene sulphonic acid

DTT:

Dithiothreitol

ph:

post-hatching

T4:

thyroxine

T3:

triiodothyronine

rT3:

reverse triiodothyronine

T2:

diiodothyronine

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Authors and Affiliations

  1. Instituto de Neurobiología, Universidad Nacional Autónoma de México, Campus Juriquilla, Querétaro, Qro, 76230, México

    A. Orozco & C. Valverde-R

  2. División de Estudios de Posgrado, Facultad de Medicina ’Dr. Ignacio Chavez’ UMSNH Morelia, Mich, México

    B. Fenton

Authors
  1. A. Orozco
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  2. B. Fenton
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  3. C. Valverde-R
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Correspondence to A. Orozco.

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Orozco, A., Fenton, B. & Valverde-R, C. Temporal profile of the outer- and inner-ring iodothyronine deiodinase pathways in the liver and skin in developing rainbow trout Oncorhynchus mykiss. Fish Physiol Biochem 29, 297–303 (2003). https://doi.org/10.1007/s10695-004-7303-7

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Keywords

  • iodothyronine deiodinases
  • thyroid hormones

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