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Does urea reabsorption occur via the glucose pathway in the kidney of the freshwater rainbow trout?

Fish Physiology and Biochemistry volume 30, pages 1–12 (2004)Cite this article

Abstract

This study tested the hypothesis that the renal reabsorption of urea occurs via the glucose transport pathway in the freshwater rainbow trout (Oncorhynchus mykiss). The relationship between glucose transport and urea transport was examined by experimentally elevating the rate of renal glucose reabsorption via infusion of the fish with exogenous glucose, and by inactivating the glucose transporters via the administration of phlorizin. Under all treatments, urea was reabsorbed against a concentration gradient, with plasma levels of urea being higher than urine levels. Glucose was almost completely reabsorbed (88%) whereas urea was reabsorbed less efficiently (47%) but to a greater extent than water (22%). Glucose and urea reabsorption were both found to be correlated with Na+ and Cl− reabsorption, though the latter were 20 fold and 200–300 fold higher than glucose and urea transport rates, respectively. Glucose infusions greatly increased glucose reabsorption but urea reabsorption was unaffected. Phlorizin treatment completely blocked glucose reabsorption, but urea reabsorption was again unaffected. We conclude that there is no relationship between glucose and urea handling in the trout kidney, thus disproving the hypothesis.

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Authors and Affiliations

  1. McMaster University, 1280 Main St. West, Hamilton, Ont., Canada, L8S 4K1

    Carol Bucking & Chris M. Wood

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  1. Carol Bucking
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  2. Chris M. Wood
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Correspondence to Carol Bucking.

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Bucking, C., Wood, C.M. Does urea reabsorption occur via the glucose pathway in the kidney of the freshwater rainbow trout?. Fish Physiol Biochem 30, 1–12 (2004). https://doi.org/10.1007/s10695-004-4154-1

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  • DOI: https://doi.org/10.1007/s10695-004-4154-1

Keywords

  • Na+ reabsorption
  • O.mykiss
  • phlorizin
  • renal function
  • urea transporters
  • urine

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