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Alcohol treatment promotes conversion of testosterone to estradiol-17β in female tilapia, Oreochromis mossambicus

Fish Physiology and Biochemistry volume 29, pages 263–268 (2003)Cite this article

Abstract

Effect of ethanol treatment on production of sex steroid hormoneswas assessed in the present study. Intraperitoneal administration (1.16 μl/g body weight) of ethanol to mature female tilapia, Oreochromis mossambicus, had little effect on plasma level of estradiol-17β (E2), but resulted in a significant decrease in plasma level of testosterone (T) at 6 days after administration. Ratio of E2 to T increased significantly from 3 days after ethanol administration. When pieces of ovaries from mature female tilapia were incubated with different doses of ethanol (0, 0.58, 2.9 and 5.8 μl/ml medium), significantly higher E2 and lower T productions were observed at 48 h in the higher ethanol treatments (2.9 and 5.8 μl/ml medium). This steroid production at higher ethanol concentration coincided with a significantly higher cytochrome P450 aromatase gene (P450arom) mRNA abundance as assessed by reverse transcription-polymerase chain reaction. Taken together, these results suggest that alcohol activates P450arom activity and promotes the conversion of T to E2 in the ovary of tilapia.

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Authors and Affiliations

  1. Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus, 3422 Sesoko, Motobu, Okinawa, 905-0227, Japan

    B. H. Kim, M. S. Rahman & A. Takemura

  2. Department of Life Science, Ara-1, Cheju National University, Jeju, 690-756, Korea

    S. J. Kim

  3. Marine and Environmental Research Institute, Hamdeok, Jocheon, Cheju National Univertisy, Jeju, 695-810, Korea

    Y. D. Lee

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  1. B. H. Kim
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  2. M. S. Rahman
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  3. S. J. Kim
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  4. Y. D. Lee
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  5. A. Takemura
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Correspondence to A. Takemura.

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Kim, B.H., Rahman, M.S., Kim, S.J. et al. Alcohol treatment promotes conversion of testosterone to estradiol-17β in female tilapia, Oreochromis mossambicus. Fish Physiol Biochem 29, 263–268 (2003). https://doi.org/10.1007/s10695-004-3268-9

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  • DOI: https://doi.org/10.1007/s10695-004-3268-9

Keywords

  • alcohol
  • aromatase
  • estradiol-17β
  • fish
  • testosterone
  • tilapia

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