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Familial Cancer

, Volume 16, Issue 4, pp 517–524 | Cite as

Genetic polymorphisms of NFκB1-94ins/delATTG and NFκBIA-881A/G genes in Egyptian patients with colorectal cancer

  • Mohamed Ragab YoussefEmail author
  • Zeinab Ibraheim Attia
  • Rizk Ahmed El-Baz
  • Sameh Roshdy
  • Ahmad Settin
Original Article

Abstract

To assess the association of genetic polymorphisms of NFκB1 and NFκBIA genes with the susceptibility to colorectal cancer (CRC). Subjects included 100 Egyptian patients with CRC (60 males and 40 females) in addition to 85 healthy controls (47 males and 38 females) from the same locality. For all participants, genetic polymorphisms of NFκB1-94ins/delATTG (rs28362491) and NFκBIA-881A/G (rs3138053) were detected by using restriction fragment length polymorphism polymerase chain reaction (RFLP–PCR). CRC patients showed a significantly higher frequency of the NFκB1-94ins/ins genotype than controls (30 vs. 4.7%) that was significant in the recessive (OR 17.69, 95% CI   5.41–57.82, p < 0.0001) and codominant models (OR   18.28, 95% CI   4.87–68.6, p < 0.0001). The NFκB1-94ins allele frequency was significantly higher among patients than controls (58 vs. 39%, OR  2.18, 95% CI  1.4–3.3, p = 0.0004). We also noticed that the genotype G/G of NFκBIA-881 polymorphism was present in patients (4%) while it was absent (0%) in controls with increased frequency of the NFκBIA-881G allele in patients compared to controls (23 vs. 14%, p = 0.041). These polymorphisms were more associated with smoking and advanced tumor staging. This study indicates that the NFκB1-94ins/ins genotype was associated with the risk of developing colorectal cancer in Egyptian subjects. Also, CRC cases showed an increase in the frequency of NFκBIA-881G allele but not reaching statistical significance for multiple comparisons.

Keywords

NFκB1-94ins/delATTG NFκBIA-881A/G Colorectal cancer Egypt 

Notes

Funding

The funding was provided by Tanta University.

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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  1. 1.Physiology Department, Faculty of ScienceTanta UniversityTantaEgypt
  2. 2.Zoology Department, Faculty of ScienceTanta UniversityTantaEgypt
  3. 3.Genetics Unit, Faculty of MedicineMansoura UniversityMansouraEgypt
  4. 4.Oncology CenterMansoura UniversityMansouraEgypt

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