Population consequences of mutational events: effects of antibiotic resistance on the r/K trade-off

Abstract

What are the effects of a mutational event on population dynamics? This eco-evolutionary question has relevance not only to basic biological theories but also to conservation applications. We evaluated the relationship between maximum population growth rate (r max) and carrying capacity (K) among strains of the bacterium Pseudomonas fluorescens. Each of 65 strains differed from their common ancestor by one naturally acquired phenotypic change conferring antibiotic resistance, brought about by a single mutational event, and each was grown in isolation in four environments. We found no evidence of a trade-off between r max and K. Rather, strains with rapid growth rates also had high carrying capacity, with little interaction between strain and environment. We conclude that the extensive variation in overall fitness resulting from single mutational events likely masks whatever population trade-offs may exist.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2

References

  1. Adam M, Murali B, Glenn NO, Potter SS (2008) Epigenetic inheritance based evolution of antibiotic resistance in bacteria. BMC Evol Biol 8:52. doi:10.1186/1471-2148-8-52

    Article  PubMed  Google Scholar 

  2. Bell G (2008) Selection: the mechanism of evolution, 2nd edn. Oxford University Press, Oxford

    Google Scholar 

  3. Carroll SP, Hendry AP, Reznick DN, Fox CW (2007) Evolution on ecological time-scales. Funct Ecol 21:387–393. doi:10.1111/j.1365-2435.2007.01289.x

    Article  Google Scholar 

  4. de Jong G, van Noordwijk AJ (1992) Acquisition and allocation of resources: genetic (co)variances, selection, and life histories. Am Nat 139:749–770. doi:10.1086/285356

    Article  Google Scholar 

  5. Eyre-Walker A, Keightley PD (2007) The distribution of fitness effects of new mutations. Nat Rev Genet 8:610–618. doi:10.1038/nrg2146

    Article  CAS  PubMed  Google Scholar 

  6. Gutteling EW, Doroszuk A, Riksen JAG, Prokop Z, Reszka J, Kammenga JE (2007) Environmental influence on the genetic correlations between life-history traits in Caenorhabditis elegans. Heredity 98:206–213. doi:10.1038/sj.hdy.6800929

    Article  CAS  PubMed  Google Scholar 

  7. Hawkey PM (2003) Mechanisms of quinolone action and microbial response. J Antimicrob Chemother 51:29–35. doi:10.1093/jac/dkg207

    Article  CAS  PubMed  Google Scholar 

  8. Hendry AP, Farrugia TJ, Kinnison MT (2008) Human influences on rates of phenotypic change in wild animal populations. Mol Ecol 17:20–29. doi:10.1111/j.1365-294X.2007.03428.x

    Article  PubMed  Google Scholar 

  9. Hoffmann AA, Willi Y (2008) Detecting genetic responses to environmental change. Nat Rev Genet 9:421–432. doi:10.1038/nrg2339

    Article  CAS  PubMed  Google Scholar 

  10. Jacoby GA (2005) Mechanisms of resistance to quinolones. Clin Infect Dis 41:S120–S126. doi:10.1086/428052

    Article  CAS  PubMed  Google Scholar 

  11. Jasmin J-N, Kassen R (2007) On the experimental evolution of specialization and diversity in heterogeneous environments. Ecol Lett 10:272–281. doi:10.1111/j.1461-0248.2007.01021.x

    Article  PubMed  Google Scholar 

  12. Johnson T, Barton N (2005) Theoretical models of selection and mutation on quantitative traits. Philos Trans R Soc Lond B Bio 360:1411–1425. doi:10.1098/rstb.2005.1667

    Article  CAS  Google Scholar 

  13. Johnson MTJ, Stinchcombe JR (2007) An emerging synthesis between community ecology and evolutionary biology. Trends Ecol Evol 22:250–257. doi:10.1016/j.tree.2007.01.014

    Article  PubMed  Google Scholar 

  14. Kassen R, Bataillon T (2006) Distribution of fitness effects among beneficial mutations before selection in experimental populations of bacteria. Nat Genet 38:484–488. doi:10.1038/ng1751

    Article  CAS  PubMed  Google Scholar 

  15. Lau JA (2008) Beyond the ecological: biological invasions alter natural selection on a native plant species. Ecology 89:1023–1031. doi:10.1890/06-1999.1

    Article  PubMed  Google Scholar 

  16. Luria SE, Delbrück M (1943) Mutations of bacteria from virus sensitivity to virus resistance. Genetics 28:491–511

    CAS  PubMed  Google Scholar 

  17. MacArthur RH, Wilson EO (1967) The theory of island biogeography. Princeton University Press, Princeton

    Google Scholar 

  18. Mace GM, Purvis A (2008) Evolutionary biology and practical conservation: bridging a widening gap. Mol Ecol 17:9–19. doi:10.1111/j.1365-294X.2007.03455.x

    Article  PubMed  Google Scholar 

  19. Millennium Ecosystem Assessment (2005) Ecosystems and human well-being: biodiversity synthesis. World Resources Institute, Washington

    Google Scholar 

  20. Mueller LD (1997) Theoretical and empirical examination of density-dependent selection. Annu Rev Ecol Syst 28:269–288. doi:10.1146/annurev.ecolsys.28.1.269

    Article  Google Scholar 

  21. Mueller LD, Ayala FJ (1981) Trade-off between r-selection and K-selection in Drosophila populations. Proc Natl Acad Sci USA 78:1303–1305. doi:10.1073/pnas.78.2.1303

    Article  PubMed  Google Scholar 

  22. Novak M, Pfeiffer T, Lenski RE, Sauer U, Bonhoeffer S (2006) Experimental tests for an evolutionary trade-off between growth rate and yield in E. coli. Am Nat 168:242–251. doi:10.1086/506527

    Article  PubMed  Google Scholar 

  23. Ostrowski EA, Rozen DE, Lenski RE (2005) Pleiotropic effects of beneficial mutations in Escherichia coli. Evol Int J Org Evol 59:2343–2352

    CAS  Google Scholar 

  24. Pianka ER (1970) On r- and K-selection. Am Nat 104:592–597. doi:10.1086/282697

    Article  Google Scholar 

  25. Rainey PB, Bailey MJ (1996) Physical and genetic map of the Pseudomonas fluorescens SBW25 chromosome. Mol Microbiol 19:521–533. doi:10.1046/j.1365-2958.1996.391926.x

    Article  CAS  PubMed  Google Scholar 

  26. Reznick D, Nunney L, Tessier A (2000) Big houses, big cars, superfleas and the costs of reproduction. Trends Ecol Evol 15:421–425. doi:10.1016/S0169-5347(00)01941-8

    Article  PubMed  Google Scholar 

  27. Reznick D, Bryant MJ, Bashey F (2002) r- and K-selection revisited: the role of population regulation in life-history evolution. Ecology 83:1509–1520

    Google Scholar 

  28. Roff DA, Fairbairn DJ (2007) The evolution of trade-offs: where are we? J Evol Biol 20:433–447. doi:10.1111/j.1420-9101.2006.01255.x

    Article  CAS  PubMed  Google Scholar 

  29. Rueffler C, Egas M, Metz JAJ (2006) Evolutionary predictions should be based on individual-level traits. Am Nat 168:E148–E162. doi:10.1086/508618

    Article  PubMed  Google Scholar 

  30. Sgrò CM, Hoffmann AA (2004) Genetic correlations, tradeoffs and environmental variation. Heredity 93:241–248. doi:10.1038/sj.hdy.6800532

    Article  PubMed  Google Scholar 

  31. Stearns SC (1977) The evolution of life history traits: a critique of the theory and a review of the data. Annu Rev Ecol Syst 8:145–171. doi:10.1146/annurev.es.08.110177.001045

    Article  Google Scholar 

  32. van Noordwijk AJ, de Jong G (1986) Acquisition and allocation of resources: their influence on variation in life history tactics. Am Nat 128:137–142. doi:10.1086/284547

    Article  Google Scholar 

  33. Worley AC, Houle D, Barrett SCH (2003) Consequences of hierarchical allocation for the evolution of life-history traits. Am Nat 161:153–167. doi:10.1086/345461

    Article  PubMed  Google Scholar 

  34. Zera A, Harshman LG (2001) The physiology of life history trade-offs in animals. Annu Rev Ecol Syst 32:95–126. doi:10.1146/annurev.ecolsys.32.081501.114006

    Article  Google Scholar 

  35. Zwietering MH, Jongenburger I, Rombouts FM, van’t Riet K (1990) Modeling of the bacterial growth curve. Appl Environ Microbiol 56:1875–1881

    PubMed  Google Scholar 

Download references

Acknowledgments

We thank Mufida Al-azzabi for technical assistance and Lauren Fitzsimmons and two anonymous reviewers for their constructive criticism of the manuscript. This work was supported by Natural Sciences and Engineering Research Council of Canada (NSERC) grants to J.T.K. and R.K., and a Tito Scaiano Ontario Graduate Scholarship in Science and Technology to J.M.F.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Rees Kassen.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Fitzsimmons, J.M., Schoustra, S.E., Kerr, J.T. et al. Population consequences of mutational events: effects of antibiotic resistance on the r/K trade-off. Evol Ecol 24, 227–236 (2010). https://doi.org/10.1007/s10682-009-9302-8

Download citation

Keywords

  • Pseudomonas fluorescens
  • Genotype × environment interactions
  • Fitness
  • Antibiotic resistance
  • Population dynamics
  • Contemporary evolution
  • Y-model
  • Fitness components model