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Marker-assisted gene introgression for resistance to Xanthomonas oryzae pv. oryzae in rice for the control of bacterial leaf blight

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Abstract

Bacterial leaf blight, sometimes known as BLB, is one of the most damaging diseases that may jeopardize the world’s supply of rice. It is caused by the bacterium Xanthomonas oryzae pv. oryzae (Xoo). It has caused sharp decline in production in regions of the globe that produce rice. More than 40 previously characterized resistance (R) genes enable host tolerance for diverse Xoo strains. In this study, three BLB resistant genes, including xa5, xa13, and Xa21, which have been crucial in disease prevention in Bangladesh, were introgressed into populations. These populations were created by crossing IRRI 154, a popular rice variety with a modern genetic background, with IRBB66 (resistant to BLB). Fifteen virulent bacterial isolates were used for BLB infection, and promising recombinants from the F5 and BC2F4 generations were found to be resistant. Using marker assisted selection (MAS) with gene-specific primers on generations F5 and BC2F4, we were able to find that 60 recombinant introgressed lines (RILs) had a pyramiding of BLB resistance genes. In terms of agronomic performance, the RILs outperformed both their donor and recipient parents, demonstrating that the RILs had pyramided three resistance genes, therefore conferring broad-spectrum BLB resistance. The produced BLB-resistant RILs offer substantial future development potential, either as cultivable crops or as BLB resistance donor material for use in boosting the yields of other rice lines.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors would like to express their gratitude to Dr. F H Ansarey, President, ACI Limited, Dhaka 1212, Bangladesh, for providing continual emotional support, and assistance with lab equipment.

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The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.

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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Md. Ariful Islam, Md. Moniruzzaman Hasan and Tanbin Akter. The first draft of the manuscript was written by Md. Ariful Islam and Md. Moniruzzaman Hasan and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Md. Moniruzzaman Hasan.

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Islam, M.A., Hasan, M.M., Rahman, M.A. et al. Marker-assisted gene introgression for resistance to Xanthomonas oryzae pv. oryzae in rice for the control of bacterial leaf blight. Euphytica 220, 60 (2024). https://doi.org/10.1007/s10681-024-03331-4

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