Abstract
Pea has been hitherto considered to have strict maternal inheritance of mitochondria, as most of flowering plants. Using a CAPS marker we detected a case of paternal inheritance of mitochondrial DNA (mtDNA) in the second generation of a cross between a wild and a cultivated representatives of the common pea (Pisum sativum L.). Heteroplasmy for mitochondrial and plastid DNA was detected in arrays of high throughput sequence reads and confirmed by analysis of CAPS markers in an accession of Pisum fulvum Sm. and probable heteroplasmy for mtDNA marker was detected in an accession of the wild P. sativum subsp, elatius. A case of mtDNA recombination in one of accessions of the same subspecies from Turkey was observed as a ca 13 Kb long insert originating from a distant evolutionary lineage. Thus, three phenomena described in this work, occasional paternal leakage of mitochondria, naturally occurring heteroplasmy and recombination of diverged mitochondrial genomes in pea, elucidated microevolutionary processes which shaped diversity in this important crop.
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Acknowledgements
Bioinformatic analysis was carried out at Computational Facilities of Siberian Supercomputer Center and of Novosibirsk State University. This work was supported by the scientific program FWNR-2022-0017 ‘Genetic control of development and formation of valuable characters in crop plants’ by the Ministry of Science and High Education.
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This work was supported by the scientific program FWNR-2022-0017 ‘Genetic control of development and forming valuable characters in crop plants’ of the Ministry of Science and High Education.
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VSB(B) assembled and analyzed mitochondrial genomes, worked out experimental design. performed the crossing experiment, scored molecular markers, analysed the data and jointly with OEK wrote the text; NVS isolated organellar DNA and prepared samples for high throughput sequencing, OEK jointly with VSB(B) wrote the text; GVV performed high throughput sequencing.
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Bulgakova (Bogdanova), V.S., Shatskaya, N.V., Kosterin, O.E. et al. Cases of paternal inheritance and recombination of mitochondrial DNA in peas (Pisum L.). Euphytica 219, 74 (2023). https://doi.org/10.1007/s10681-023-03207-z
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DOI: https://doi.org/10.1007/s10681-023-03207-z