Abstract
Agropyron cristatum (L.) Gaertn. (2n = 4x = 28, PPPP), which is a wild relative of common wheat and has a P genome, has many excellent characteristics (for example, it is disease-resistant, stress-resistant, and high yielding) and has important utilization values for the genetic improvement of wheat. In this study, the wheat–A. cristatum 3P addition line I-27 and 3P(3B) substitution line I-15 were identified for the first time via in situ hybridization (ISH) and specific molecular marker (EST-STS) from wheat–A. cristatum-derived lines, which are a representative and important material for the utilization of the excellent genes from the A. cristatum chromosome 3P for wheat genetic improvement. In terms of agronomic traits, the tiller number and spike length of the 3P addition line I-27 were significantly higher than those of Fukuhokomugi. The spike length and spikelet number per spike of the 3P substitution line I-15 were significantly greater than those of Fukuho. Additionally, the wheat–A. cristatum 3P substitution line I-15 was resistant to wheat leaf rust. The generation of the 3P addition line I-27 and the 3P substitution line I-15 was essential for the transfer of the excellent genes of the A. cristatum 3P chromosome into common wheat, and resulted in the wheat–A. cristatum addition line containing a complete set of genetic research materials, laying the foundation for comparative studies of the P genome and subsequent fine mapping.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bartos P, Dyck PL, Samborski DJ (1969) Adult-plant leaf rust resistance in Thatcher and Marquis wheat: a genetic analysis of the host-parasite interaction. Can J Bot 47:267–269. https://doi.org/10.1139/b69-039
Chapman V, Riley R (1955) Disomic addition of rye chromosome II to wheat. Nature 175:1091–1092. https://doi.org/10.1038/1751091a0
Chen PD, Qi LL, Zhou B, Zhang SZ, Liu DJ (1995) Development and molecular cytogenetic analysis of wheat-Haynaldia villosa 6VS/6AL translocation lines specifying resistance to powdery mildew. Theor Appl Genet 91:1125–1128. https://doi.org/10.1007/BF00223930
Chen PD, Liu WX, Yuan JH, Wang X, Zhou B, Wang SL, Zhang SZ, Feng YG, Yang BJ, Liu GX, Liu DJ, Qi LL, Zhang P, Friebe B, Gill BS (2005) Development and characterization of wheat-Leymus racemosus translocation lines with resistance to Fusarium head blight. Theor Appl Genet 111:941–948. https://doi.org/10.1007/s00122-005-0026-z
Chen ZY, Cheng XJ, Chai LL, Wang ZH, Du DJ, Wang ZH, Bian RL, Zhao AJ, Xin MM, Guo WL, Hu ZR, Peng HR, Yao YY, Sun QX, Ni ZF (2020) Pleiotropic QTL influencing spikelet number and heading date in common wheat (Triticum aestivum L.). Theor Appl Genet 133:1825–1838. https://doi.org/10.1007/s00122-020-03556-6
Chen ZY, Ke WS, He F, Chai LL, Cheng XJ, Xu HW, Wang XB, Du DJ, Zhao YD, Chen XY, Xing JW, Xin MM, Guo WL, Hu ZR, Su ZQ, Liu J, Peng HR, Yao YY, Sun QX, Ni ZF (2022) A single nucleotide deletion in the third exon of FT-D1 increases the spikelet number and delays heading date in wheat (Triticum aestivum L.). Plant Biotechnol J 20:920–933. https://doi.org/10.1111/pbi.13773
Darko E, Khalil R, Dobi Z, Kovacs V, Szalai G, Janda T, Molnar I (2020) Addition of Aegilops biuncialis chromosomes 2M or 3M improves the salt tolerance of wheat in different way. Sci Rep 10:22327–22336. https://doi.org/10.1038/s41598-020-79372-1
Dewey DR (1969) Synthetic hybrids of Agropyron caespitosum×Agropyron spicatum, Agropyron caninum, and Agropyron yezoense. Bot Gazette 130:110–116. https://doi.org/10.1002/j.1537-2197.1968.tb07479.x
Dewey DR (1984) The genomic system of classification as a guide to intergeneric hybridization with the perennial Triticeae. In: Gustafson JP (ed) Gene manipulation in plant improvement. Springer, New York, pp 209–279
Dobrovolskaya O, Martinek P, Voylokov AV, Korzun V, Roder MS, Borner A (2009) Microsatellite mapping of genes that determine supernumerary spikelets in wheat (T. aestivum) and rye (S. cereale). Theor Appl Genet 119:867–874. https://doi.org/10.1007/s00122-009-1095-1
Du DJ, Zhang DX, Yuan J, Feng M, Li ZJ, Wang ZH, Zhang ZH, Li XT, Ke WS, Li RH, Chen ZY, Chai LL, Hu ZR, Guo WL, Xing JW, Su ZQ, Peng HR, Xin MM, Yao YY, Sun QX, Liu J, Ni ZF (2021) FRIZZY PANICLE defines a regulatory hub for simultaneously controlling spikelet formation and awn elongation in bread wheat. New Phytol 231:814–833. https://doi.org/10.1111/nph.17388
Friebe B, Schubert V, Biuthner WD, Hammer K (1992) C-banding pattern and polymorphism of Aegilops caudata and chromosome constitutions of the amphiploid T. aestivum – Ae. Caudate and six derived chromosome addition lines. Theor Appl Genet 83:589–596. https://doi.org/10.1007/BF00222072
Guo CH, Yang L, Liu CY (2011) Study on chromosome aberrations of crosses between 3E disomic addition line and 2C addition line. Adv Mater Res 356:236–239
Guo J, Zhang XL, Hou YL, Cai JJ, Shen XR, Zhou TT, Xu HH, Ohm HW, Wang HW, Li AF, Han FP, Wang HG, Kong LR (2015) High-density mapping of the major FHB resistance gene Fhb7 derived from Thinopyrum ponticum and its pyramiding with Fhb1 by marker-assisted selection. Theor Appl Genet 128:2301–2316. https://doi.org/10.1007/s00122-015-2586-x
Han HM, Liu WH, Lu YQ, Zhang JP, Yang XM, Li XQ, Hu ZM, Li LH (2017) Isolation and application of P genome-specific DNA sequences of Agropyron Gaertn. in Triticeae. Planta 245:425–437. https://doi.org/10.1007/s00425-016-2616-1
Han HM, Liu WH, Zhang JP, Zhou SH, Yang XM, Li XQ, Li LH (2019) Identification of P genome chromosomes in Agropyron cristatum and wheat-A. cristatum derivative lines by FISH. Sci Rep 9:9712–9720. https://doi.org/10.1038/s41598-019-46197-6
Hunter MC, Smith RG, Schipanski ME, Atwood LW, Mortensen DA (2017) Agriculture in 2050: recalibrating targets for sustainable intensification. Bioscience 67:385–390. https://doi.org/10.1093/biosci/bix010
Jiang B, Liu TG, Li HG, Han HM, Li LH, Zhang JP, Yang XM, Zhou SH, Li XQ, Liu WH (2018) Physical mapping of a novel locus conferring leaf rust resistance on the long arm of Agropyron cristatum chromosome 2P. Front Plant Sci 9:817–829. https://doi.org/10.3389/fpls.2018.00817
Kang HY, Wang Y, Fedak G, Cao WG, Zhang HQ, Fan X, Sha LN, Xu LL, Zheng YL, Zhou YH (2011) Introgression of chromosome 3Ns from Psathyrostachys huashanica into wheat specifying resistance to stripe rust. PLoS ONE 6:e21802. https://doi.org/10.1371/journal.pone.0021802
Katz A, Byrne P, Reid S, Bratschun S, Haley S, Pearce S (2022) Identification and validation of a QTL for spikelet number on chromosome arm 6BL of common wheat (Triticum aestivum L.). Mol Breed 42:17–33. https://doi.org/10.1007/s11032-022-01288-7
Kokhmetova A, Rsaliyev S, Atishova M, Kumarbayeva M, Malysheva A, Keishilov Z, Zhanuzak D, Bolatbekova A (2021) Evaluation of wheat germplasm for resistance to leaf rust (Puccinia triticina) and identification of the sources of Lr resistance genes using molecular markers. Plants 10:1484–1497. https://doi.org/10.3390/plants10071484
Kolmer JA, Anderson JA, Flor JM (2010) Chromosome location, linkage with simple sequence repeat markers, and leaf rust resistance conditioned by gene in wheat. Crop Sci 50:2392–2395. https://doi.org/10.2135/cropsci2010.01.0005
Kolmer JA, Su ZQ, Bernardo A, Bai GH, Chao SM (2018) Mapping and characterization of the new adult plant leaf rust resistance gene Lr77 derived from Santa Fe winter wheat. Theor Appl Genet 131:1553–1560. https://doi.org/10.1007/s00122-018-3097-3
Kolodziej MC, Singla J, Sanchez-Martin J, Zbinden H, Simkova H, Karafiatova M, Dolezel J, Gronnier J, Poretti M, Glauser G, Zhu W, Koster P, Zipfel C, Wicker T, Krattinger SG, Keller B (2021) A membrane-bound ankyrin repeat protein confers race-specific leaf rust disease resistance in wheat. Nat Commun 12:956–967. https://doi.org/10.1038/s41467-020-20777-x
Kuzay S, Xu YF, Zhang JL, Katz A, Pearce S, Su ZQ, Fraser M, Anderson JA, Brown-Guedira G, DeWitt N, Haugrud AP, Faris JD, Akhunov E, Bai GH, Dubcovsky J (2019) Identification of a candidate gene for a QTL for spikelet number per spike on wheat chromosome arm 7AL by high-resolution genetic mapping. Theor Appl Genet 132:2689–2705. https://doi.org/10.1007/s00122-019-03382-5
Li H, Wang X (2009) Thinopyrum ponticum and Th. intermedium: the promising source of resistance to fungal and viral diseases of wheat. J Genet Genom 36:557–565. https://doi.org/10.1016/S1673-8527(08)60147-2
Li LH, Li XQ, Li P, Dong YC, Zhao GS (1997) Establishment of wheat-Agropyron cristatum alien addition lines I. Cytology of F3, F2BC1, BC4, and BC3F1 progenies. Acta Genet Sin 24:154–159
Li LH, Yang XM, Zhou RH, Li XQ, Dong YC (1998) Establishment of wheat-Agropyron cristatum alien addition lines II. Identification of alien chromosomes and analysis of development approaches. Acta Genet Sin 25:538–544
Li HH, Lv MJ, Song LQ, Zhang JP, Gao AN, Li LH, Liu WH (2016) Production and identification of wheat-Agropyron cristatum 2P translocation lines. PLoS ONE 11:e0145928. https://doi.org/10.1371/journal.pone.0145928
Li QF, Lu YQ, Pan CL, Wang ZJ, Liu FL, Zhang JP, Yang XM, Li XQ, Liu WH, Li LH (2020) Characterization, identification and evaluation of a novel wheat-Agropyron cristatum(L.) Gaertn. disomic addition line II-30-5. Genet Resour Crop Ev 67:2213–2223. https://doi.org/10.1007/s10722-020-00975-8
Li T, Deng GB, Tang YY, Su Y, Wang JH, Cheng J, Yang Z, Qiu XB, Pu X, Zhang HL, Liang JJ, Yu MQ, Wei YM, Long H (2021) Identification and validation of a novel locus controlling spikelet number in bread wheat (Triticum aestivum L.). Front Plant Sci 12:611106. https://doi.org/10.3389/fpls.2021.611106
Liu WH, Luan Y, Wang JC, Wang XG, Su JJ, Zhang JP, Yang XM, Gao AN, Li LH (2010) Production and identification of wheat-Agropyron cristatum (1.4P) alien translocation lines. Genome 53:472–481. https://doi.org/10.1139/G10-023
Luan Y, Wang X, Liu W, Li C, Zhang J, Gao A, Wang Y, Yang X, Li L (2010) Production and identification of wheat-Agropyron cristatum 6P translocation lines. Planta 232:501–510. https://doi.org/10.1007/s00425-010-1187-9
Marais GF, Bekker TA, Eksteen A, Mccallum B, Fetch T, Marais AS (2010) Attempts to remove gametocidal genes co-transferred to common wheat with rust resistance from Aegilops speltoides. Euphytica 171:71–85. https://doi.org/10.1007/s10681-009-9996-2
Pan CL, Li QF, Lu YQ, Zhang JP, Yang XM, Li XQ, Li LH, Liu WH (2017) Chromosomal localization of genes conferring desirable agronomic traits from Agropyron cristatum chromosome 1P. PLoS ONE 12:0175265. https://doi.org/10.1371/journal.pone.0175265
Pan CL, Li QF, Han HM, Zhang JP, Zhou SH, Yang XM, Li XQ, Li LH, Liu WH (2022) Identification of 5P chromosomes in Wheat-Agropyron cristatum addition line and analysis of its effect on homologous pairing of wheat chromosomes. Front Plant Sci 13:844348. https://doi.org/10.3389/fpls.2022.844348
Pang YH, Chen XH, Zhao JX, Du WL, Cheng XN, Wu J, Li YL, Wang LM, Wang J, Yang QH (2014) Molecular cytogenetic characterization of a wheat-Leymus mollis 3D(3Ns) substitution line with resistance to leaf rust. J Genet Genom 41:205–214. https://doi.org/10.1016/j.jgg.2013.11.008
Qi K, Han HM, Zhang JP, Zhou SH, Li XQ, Yang XM, Liu WH, Lu YQ, Li LH (2021) Development and characterization of novel Triticum aestivum-Agropyron cristatum 6P robertsonian translocation lines. Mol Breed 41:59–71. https://doi.org/10.1007/s11032-021-01251-y
Qin JY, Li ZF, Yan XC, Su JH, Liu DQ (2015) Molecular identification of leaf rust resistance gene in wheat line 5R625. Acta Agron Sin 41:651. https://doi.org/10.3724/SP.J.1006.2015.00651
Qureshi N, Bariana H, Kumran VV, Muruga S, Forrest KL, Hayden MJ, Bansal U (2018) A new leaf rust resistance gene Lr79 mapped in chromosome 3BL from the durum wheat landrace Aus26582. Theor Appl Genet 131:1091–1098. https://doi.org/10.1007/s00122-018-3060-3
Rey E, Abrouk M, Keeble-Gagnere G, Karafiatova M, Vrana J, Balzergue S, Soubigou-Taconnat L, Brunaud V, Martin-Magniette ML, Endo TR, Bartos J, Appels R, Dolezel J, Cons IWGS (2018) Transcriptome reprogramming due to the introduction of a barley telosome into bread wheat affects more barley genes than wheat. Plant Biotechnol J 16:1767–1777. https://doi.org/10.1111/pbi.12913
Roelfs AP, Singh RP, Saari EE (1992) Rust diseases of wheat: concepts and methods of disease management. Plant Diseases. pp: 76–81
Savary S, Willocquet L, Pethybridge SJ, Esker P, McRoberts N, Nelson A (2019) The global burden of pathogens and pests on major food crops. Nat Ecol Evol 3:430–439. https://doi.org/10.1038/s41559-018-0793-y
Schneider A, Linc G, Molnar I, Molnar-Lang M (2005) Molecular cytogenetic characterization of Aegilops biuncialis and its use for the identification of 5 derived wheat-Aegilops biuncialis disomic addition lines. Genome 48:1070–1082. https://doi.org/10.1139/G05-062
Singh RP, McIntosh RA (1984) Complementary genes for reaction to Puccinia recondita tritici in Triticum aestivum L. Genetic and linkage studies. Canjgenetcytol 26:736–742. https://doi.org/10.1139/g84-115
Song LQ, Zhao H, Zhang Z, Zhang S, Liu JJ, Zhang W, Zhang N, Ji J, Li LH, Li JM (2020) Molecular cytogenetic identification of wheat-Aegilops biuncialis 5M(b) disomic addition line with tenacious and black glumes. Int J Mol Sci 21:4053–4065. https://doi.org/10.3390/ijms21114053
Su ZQ, Bernardo A, Tian B, Chen H, Wang S, Ma HX, Cai SB, Liu DT, Zhang D, Li T, Trick H, Amand P, Yu JM, Zhang ZY, Bai GH (2019) A deletion mutation in TaHRC confers Fhb1 resistance to Fusarium head blight in wheat. Nat Genet 51:1–7. https://doi.org/10.1038/s41588-019-0425-8
Sun YY, Lyu MJ, Han HM, Zhou SH, Lu YQ, Liu WH, Yang XM, Li XQ, Zhang JP, Liu X, Li LH (2021) Identification and fine mapping of alien fragments associated with enhanced grain weight from Agropyron cristatum chromosome 7P in common wheat backgrounds. Theor Appl Genet 134:3759–3772. https://doi.org/10.1007/s00122-021-03927-7
Tang ZX, Li M, Chen L, Wang YY, Ren Z, Fu SL (2014a) New types of wheat chromosomal structural variations in derivatives of wheat-rye hybrids. PLoS ONE 9:e110282. https://doi.org/10.1371/journal.pone.0110282
Tang ZX, Yang ZJ, Fu SL (2014b) Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis. J Appl Genet 55:313–318. https://doi.org/10.1007/s13353-014-0215-z
Wang HW, Sun SL, Ge WY, Zhao LF, Hou BQ, Wang K, Lyu ZF, Chen LY, Xu SS, Guo J, Li M, Su PS, Li XF, Wang GP, Bo CY, Fang XJ, Zhuang WW, Cheng XX, Wu JW, Dong LH, Chen WY, Li W, Xiao GL, Zhao JX, Hao YC, Xu Y, Gao Y, Liu WJ, Liu YH, Yin HY, Li JZ, Li X, Zhao Y, Wang XQ, Ni F, Ma X, Li AF, Xu SS, Bai GH, Nevo E, Gao CX, Ohm H, Kong LR (2020) Horizontal gene transfer of Fhb7 from fungus underlies Fusarium head blight resistance in wheat. Science 368(6493):5435. https://doi.org/10.1126/science.aba5435
Wang X, Han BH, Sun YY, Kang XL, Zhang M, Han HM, Zhou SH, Liu WH, Lu YQ, Yang XM, Li XQ, Zhang JP, Liu X, Li LH (2022) Introgression of chromosome 1P from Agropyron cristatum reduces leaf size and plant height to improve the plant architecture of common wheat. Theor Appl Genet 135:1951–1963. https://doi.org/10.1007/s00122-022-04086-z
Wu J, Yang XM, Wang H, Li HJ, Li LH, Li XQ, Liu WH (2006) The introgression of chromosome 6P specifying for increased numbers of florets and kernels from Agropyron cristatum into wheat. Theor Appl Genet 114:13–20. https://doi.org/10.1007/s00122-006-0405-0
Yang WJ, Wang CY, Chen CH, Wang YJ, Zhang H, Liu XL, Ji WQ (2016) Molecular cytogenetic identification of a wheat-rye 1R addition line with multiple spikelets and resistance to powdery mildew. Genome 59:277–288. https://doi.org/10.1139/gen-2015-0129
Yang XF, Li X, Wang CY, Chen CH, Tian ZR, Ji WQ (2017) Isolation and molecular cytogenetic characterization of a wheat-Leymus mollis double monosomic addition line and its progenies with resistance to stripe rust. Genome 60:1029–1036. https://doi.org/10.1139/gen-2017-0078
Ye XL, Lu YQ, Liu WH, Chen GY, Han HM, Zhang JP, Yang XM, Li XQ, Gao AN, Li LH (2015) The effects of chromosome 6P on fertile tiller number of wheat as revealed in wheat-Agropyron cristatum chromosome 5A/6P translocation lines. Theor Appl Genet 128:797–811. https://doi.org/10.1007/s00122-015-2466-4
Yuan C, Singh RP, Liu D, Randhawa MS, Huerta-Espino J, Lan C (2020) Genome-wide mapping of adult plant resistance to leaf rust and stripe rust in CIMMYT wheat line Arableu#1. Plant Dis 104:1455–1464. https://doi.org/10.1094/PDIS-10-19-2198-RE
Zhang JP, Liu WH, Lu YQ, Liu QX, Yang XM, Li XQ, Li LH (2017) A resource of large-scale molecular markers for monitoring Agropyron cristatum chromatin introgression in wheat background based on transcriptome sequences. Sci Rep 7:11942–11950. https://doi.org/10.1038/s41598-017-12219-4
Zhang J, Jiang Y, Wang Y, Guo YL, Long H, Deng GB, Chen Q, Xuan P (2018) Molecular markers and cytogenetics to characterize a wheat-Dasypyrum villosum 3V(3D) substitution line conferring resistance to stripe rust. PLoS ONE 13:0202033. https://doi.org/10.1371/journal.pone.0202033
Zhang RQ, Xiong CAX, Mu HQ, Yao RN, Meng XR, Kong LN, Xing LP, Wu JZ, Feng YG, Cao AZ (2021) Pm67, a new powdery mildew resistance gene transferred from Dasypyrum villosum chromosome 1V to common wheat (Triticum aestivum L.). Crop J 9:882–888. https://doi.org/10.1016/j.cj.2020.09.012
Zhao JX, Liu Y, Cheng XN, Pang YH, Li JC, Su ZQ, Wu J, Yang QH, Bai GH, Chen XH (2019) Development and identification of a dwarf wheat-Leymus mollis double substitution line with resistance to yellow rust and Fusarium head blight. Crop J 7:516–526. https://doi.org/10.1007/s10681-012-0772-3
Zhou Y, Zhao XB, Li YW, Xu J, Bi AY, Kang LP, Xu DX, Chen HF, Wang Y, Wang YG, Liu SY, Jiao CZ, Lu HF, Wang J, Yin CB, Jiao YL, Lu F (2020) Triticum population sequencing provides insights into wheat adaptation. Nat Genet 52:1412–1422. https://doi.org/10.1038/s41588-020-00722-w
Funding
This research was supported by Grants from the National Key Research and Development Program of China (2016YFD0100102).
Author information
Authors and Affiliations
Contributions
WL and LL designed the study. ZK, SX and XJ performed the experiment. ZK wrote the manuscript. HH, SZ, JZ, XY and XL provided materials and reagents for the study. All authors have read and agreed to the published version of the manuscript.
Corresponding authors
Ethics declarations
Conflict of interest
The authors declare no conflict of interest.
Data availability
All data generated or analyzed during this study are included in this published article.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Kuang, Z., Ji, X., Xu, S. et al. Generation and identification of a wheat–Agropyron cristatum (L.) Gaertn. 3P chromosome addition line and substitution line. Euphytica 219, 23 (2023). https://doi.org/10.1007/s10681-022-03149-y
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s10681-022-03149-y