Abstract
Phytic acid (PA) is an important antinutritional component in maize that affects the availability of major micro-nutrients like di- and multi-valent mineral cations like iron (Fe) and zinc (Zn). The long-term consumption of maize as a staple food crop leads to micronutrient malnutrition especially iron and zinc deficiency in the human population. In addition, it also acts as a storehouse of a major part of mineral phosphorous (P), approximately 80% of the total P stored as phytate P is not available to mono-gastric animals like humans and poultry birds, and it gets excreted as such, leading to one of the major environmental pollution called eutrophication. Of the various low phytic acid (lpa) mutants, lpa2-2 generated through mutagenesis reduces PA by 30%. BML 6 and BML 45, the parents of the popular maize hybrid DHM 121 with high PA were selected to introgress lpa2-2 through marker-assisted backcross breeding (MABB). The percent recurrent parental genome (RPG) in the selected BC2F2 plants ranged from 88.68 to 91.04% and 90.09–91.51% in the genetic background of BML 6 and BML 45, respectively. Based on the highest percentage of RPG, best five BC2F2 plants, viz., #3190, #3283, #3230, #3263 and #3292 with RPG 88.68–91.04% in the genetic background of BML 6 and #3720, #3776, #3717, #3828 and #3832 with RPG 90.09–91.51% in the genetic background of BML 45 were advanced to BC2F3. The newly developed near-isogenic lines (NILs) possessed low phytate content (2.37 mg/g in BML 6 and 2.40 mg/g in BML 45) compared to 3.59 mg/g and 3.16 mg/g in recurrent parents BML 6 and BML 45, respectively thereby reducing the phytate by an average of 34 and 24 per cent, respectively. These newly developed progenies were similar to their recurrent parents for various morphological traits. These inbreds assume great significance in alleviating Fe and Zn deficiencies in worldwide.
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The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
The authors acknowledge Dr. R. K. Khulbe and ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora for sharing lpa2 donor under CRPMB Project. The author also acknowledge the PJTSAU, Hyderabad for sharing the recurrent parental lines BML 6 and BML 45 for conversion through MABB, The author also acknowledge Dr. A. K. Singh, Director, ICAR-Indian Agricultural Research Institute and also the Project Coordinator, CRPMB and Dr. D. K. Yadava, ADG (Seed), ICAR for their support and guidance while undertaking the project.
Funding
This work is supported by the Indian Council of Agricultural Research (ICAR) under Consortium Research Platform on Molecular Breeding (CRPMB) Project.Indian Council of Agricultural Research
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Conceptualization and investigation of work by CGK and YKR. The experiments and studies are conducted by YKR, SSG. Part of the experimental work was also supported by AK, P J, H K Y, and S S. The data analysis was done by HK MS and SK. The first draft of the manuscript was written by YKR and CGK. The manuscript was imporved by SR, AS, JCS, RNG, RKP, FH, and AKD. All authors read and approved the manuscript.
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Yathish, K.R., Karjagi, C.G., Gangoliya, S.S. et al. Introgression of the low phytic acid locus (lpa2) into elite maize (Zea mays L.) inbreds through marker-assisted backcross breeding (MABB). Euphytica 218, 127 (2022). https://doi.org/10.1007/s10681-022-03076-y
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DOI: https://doi.org/10.1007/s10681-022-03076-y