Abstract
The influence of Fusarium wilt in lettuce (Lactuca sativa L.) is becoming increasingly severe under the influence of global warming in tropical and subtropical regions. Previously, we reported on the development of a PCR-based marker, LG1_v8_116.506Mbp, on LG1 for the resistance to Fusarium wilt race 2. This study investigated genetic resources with novel resistance to race 2 using molecular markers for stable lettuce production. Comparing genotypes and phenotypes of the 48 cultivars/lines, it was found that ‘CGN17397’ and ‘2008–11’, which were at first considered to possess the same resistance genes, showed resistant phenotypes but had susceptible genotypes in the marker on LG1. Allelism tests using ten resistant cultivars/lines showed that the resistance derived from ‘CGN17397’ was not located on LG1. Using the two F2 populations derived from ‘CGN17397’, it was clarified that the resistance was controlled by a single semi-dominant gene located at the region between 63.476 Mbp and 66.012 Mbp on LG7, defined by AFLP analysis and fine mapping with PCR-based markers. Of the four markers which showed complete co-segregation with the resistance phenotype, only LG7_v8_65.645Mbp allowed us to identify the resistance genotype by genotyping the 48 cultivars/lines. According to the annotation of the reference genome sequence of ‘Salinas’, the locus for resistance on LG7 had twelve disease resistance gene candidates. The elucidation of underlying resistance mechanisms in the two loci on LG1 and LG7 is of future concern. These results may contribute to accelerating the breeding of Fusarium wilt-resistant cultivars and add understanding to the fundamental mechanisms for resistance to race 2 in lettuce.
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Acknowledgements
The authors would like to thank Dr. Toshiyuki Usami, Chiba University, for helpful discussions and advice.
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The present research was supported by Nagano Prefecture, Japan.
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KS and YS contributed equally to the present work. KS, HS, HS and TK planned the experiments. KS and HS performed the development of the mapping populations and the infection assay for disease resistance. MH, YS, HS, SK, AT and TK performed sequence analysis. KS and ES performed genotyping analysis. YS, MH, AT, FC and TK performed AFLP analysis. KS and TK wrote the manuscript. All authors read and approved the final manuscript.
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Seki, K., Shimizu, Y., Hayashi, M. et al. Discovery and fine mapping of a novel resistance locus to Fusarium wilt race 2 in lettuce (Lactuca sativa L.). Euphytica 218, 120 (2022). https://doi.org/10.1007/s10681-022-03058-0
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DOI: https://doi.org/10.1007/s10681-022-03058-0