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Behavior of chromosomes from the Mexican wild diploid species Solanum pinnatisectum in the interspecific hybrid with cultivated potato and its backcross progenies

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Abstract

The genomic relationship between Mexican wild diploid species and South American wild and cultivated potato species is ambiguous because these species are reproductively isolated from each other. Previously, we successfully obtained triploid interspecific hybrids between a chromosome-doubled clone of the Mexican wild diploid species Solanum pinnatisectum (2n = 2x = 24) and a cultivated diploid species (A-genome species); these hybrids were then chromosome-doubled and backcrossed to tetraploid potatoes. In this study, we conducted a genomic in situ hybridization (GISH) analysis to reveal the chromosome behaviors of the S. pinnatisectum chromosomes in the hybrid progenies. Due to the high specificity of the A-genome probe for A-genome chromosomes, the chromosomes of the two species were differentially painted. In the triploid hybrid, bivalents with two S. pinnatisectum chromosomes and univalents with cultivated species chromosomes were exclusively observed at meiosis. However, in the hexaploid hybrid, 20% of the S. pinnatisectum chromosomes showed allosyndetic pairing with A-genome chromosomes and formed bivalents and multivalents. From the BC1 to BC4 plants, the number of S. pinnatisectum chromosomes was drastically reduced from 24 to an average of 0.9, and translocation chromosomes were observed. Therefore, we conclude that the S. pinnatisectum germplasm was actually introgressed by recombination into the S. tuberosum genome, which was demonstrated by the presence of an S. pinnatisectum-derived late blight resistance gene (Rpi-blb3) in BC4 plants whose chromosomes were all painted with the A-genome probe.

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Data availability

All data are included in the manuscript. Plant materials are available upon request.

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Acknowledgements

We thank the US Potato Genebank at Sturgeon Bay, Wis. for providing Solanum seeds. This research was supported partly by Calbee Inc., Hokkaido Potato Growers Association, Kewpie Corp., KENKO Mayonnaise Co., Ltd., and the Japan Snack Cereal Foods Association.

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  1. Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, Matsudo, Chiba, 271-8510, Japan

    Shinji Kikuchi & Hikari Ishii

  2. Potato Germplasm Enhancement Laboratory, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan

    Kazuyoshi Hosaka & Rena Sanetomo

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  1. Shinji Kikuchi
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SK, RS and KH conceived and designed the experiment and interpreted the results. SK and HI carried out the chromosome observation and GISH analysis. RS prepared all plants and DNA materials. KH conducted the marker analysis. RS and SK wrote the manuscript with the help of KH who edited the final version. All authors read and approved the final manuscript.

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Correspondence to Rena Sanetomo.

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Kikuchi, S., Ishii, H., Hosaka, K. et al. Behavior of chromosomes from the Mexican wild diploid species Solanum pinnatisectum in the interspecific hybrid with cultivated potato and its backcross progenies. Euphytica 218, 56 (2022). https://doi.org/10.1007/s10681-022-03003-1

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