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Tagging of SSR markers associated to yellow mosaic virus resistance in black gram (Vigna mungo (L.) Hepper)

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Abstract

In black gram, yellow mosaic virus (YMV) disease is a serious problem causing severe yield reduction. In the present study, F2 and RIL Population (F4:5) of the cross IC436656 (YMV susceptible) x KKB14045 (YMV resistant) were evaluated for YMV resistance through bulked segregant analysis (BSA) using SSR markers. Out of 91 SSR markers studied, thirteen were polymorphic between IC436656 and KKB14045 which showed 14.3% of genome diversity. The F2 population segregated as 3:1 for susceptibility and resistance in chi-square test which indicated monogenic recessive gene control the YMV resistance. From single marker analysis (SMA), CEDG141 and CEDG008 were strongly associated to YMV resistance. From BSA, CEDG141 differentiated IC436656 (200 bp) and KKB14045 (280 bp) in F2 population. CEDG141, CEDG 264 and CEDG 008 differentiated the RILs of the same population in BSA. The identified SSR markers would be valuable in black gram breeding programs for YMV resistance studies.

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Abbreviations

%:

Percent

AC & RI:

Agricultural College and Research Institute

bp:

Base pair

BSA :

Bulked segregant analysis

cM:

Centimorgan

CTAB :

CetylTrimetyl ammonium bromide

DNA:

Deoxyribo nucleic acid

dNTPs:

Deoxynucleoside triphosphates

dATP:

Deoxyadenosine triphosphate

dGTP:

Deoxyguanosine triphosphate

dCTP:

Deoxycytidine triphosphate

dTTP:

Deoxythymidine triphosphate

EDTA :

Ethylene diamine tetra acetic acid

ml :

Milliliter

mM:

Millimolar

MYMIV:

Mungbean yellow mosaic Indian virus

MYMV:

Mungbean yellow mosaic virus

NBPGR:

National bureau of plant genetic resources

PCR :

Polymerase chain reaction

QTL :

Quantitative trait loci

SSR :

Simple sequence repeats

SMA :

Single marker analysis

SCAR :

Sequence characterized amplified regions

TBE:

Tris-borate EDTA

µg:

Microgram

μl:

Microliter

YMV :

Yellow mosaic virus

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Sathees, N., Shoba, D., Mani, N. et al. Tagging of SSR markers associated to yellow mosaic virus resistance in black gram (Vigna mungo (L.) Hepper). Euphytica 218, 23 (2022). https://doi.org/10.1007/s10681-022-02976-3

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