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MAGIC lines in chickpea: development and exploitation of genetic diversity

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In chickpea a multi-parent advanced generation intercross (MAGIC) population was developed using eight parents that are improved varieties and widely adaptable breeding lines. The main objective was to enhance the genetic diversity and bring novel alleles for developing superior chickpea varieties. The development scheme involved a sequence of 28 two-way, 14 four-way and 7 eight-way crosses, followed by bulking of final F1 plants. From F2 generation onwards single plants were grown as progenies and advanced to F8 by single seed descent method. The finally developed 1136 MAGIC lines were phenotyped under rainfed (RF) and irrigated (IR) conditions for 2 years (2013 and 2014) under normal season, and one year under heat stress (HS) condition (summer-2014) in field to estimate the genetic diversity created among these lines. Under RF-2014, RF-2013, IR-2014, IR-2013 and S-2014 seasons 46, 62, 83, 50 and 61 lines showed significantly higher grain yield than the best parent, respectively. Similarly, 23 and 19 common lines were identified under RF and IR conditions over two years and no common line was identified between RF/IR and HS conditions. Preliminary evaluation showed a large variation among MAGIC lines for flowering time (34–69 days), maturity (80–120 days), plant height (23.3–65 cm), grain yield (179–4554 kg/ha), harvest index (0.10–0.77) and 100 seed weight (10–45 g) under RF and IR conditions. Several genotypes with higher grain yield than the best check under heat stress were identified. These MAGIC lines provide a useful germplasm source with diverse allelic combinations to global chickpea community.

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  • Arif A, Parveen N, Waheed MQ, Atif RM, Waqar I, Shah TM (2021) A comparative study for assessing the drought-tolerance of chickpea under varying natural growth environments. Front Plant Sci 11:607869

    Article  Google Scholar 

  • Bandillo N, Muyco PA, Caspillo C, Laza M, Sajise AG, Singh RK, Gregorio GB, Redona E, Leung H (2010) Development of multiparent advanced generation intercross (MAGIC) populations for gene discovery in rice (Oryza sativa L.). Philipp J Crop Sci 35:96

    Google Scholar 

  • Basu PS, Ali M, Chaturvedi S (2009) Terminal heat stress adversely affects chickpea productivity in northern India-strategies to improve thermotolerance in the crop under climate change. In: ISPRS Archives XXXVIII-8/W3 workshop proceedings: impact of climate change on agriculture, pp 189–193

  • Bevan EH, Andrew WG, Kerrie LF, Andrzej K, Matthew JH, Matthew KM, Colin RC (2012) A multiparent advanced generation inter-cross population for genetic analysis in wheat. Plant Biotechnol J 10:826–839

    Article  Google Scholar 

  • Bharadwaj C, Tripathi S, Soren KR, Thudi M, Singh RK, Sheoran S et al (2020) Introgression of “QTL-hotspot” region enhances drought tolerance and grain yield in three elite chickpea cultivars. Plant Genome.

    Article  Google Scholar 

  • Cavanagh C, Morell M, Mackay I, Powell W (2008) From mutations to MAGIC: resources for gene discovery, validation and delivery in crop plants. Curr Opin Plant Biol 11:215–221

    Article  Google Scholar 

  • Devasirvatham V, Tan D (2018) Impact of high temperature and drought stresses on chickpea production. Agron J 8:1–9

    Google Scholar 

  • Devasirvatham V, Tan D, Trethowan RM, Gaur P, Mallikarjuna N (2010) Impact of high temperature on the reproductive stage of chickpea. In: Food security from sustainable agriculture proceedings of the 15th Australian Society of Agronomy conference, pp 15–18

  • Devasirvatham V, Gaur PM, Mallikarjuna N, Tokachichu RN, Trethowan RM, Tan DKY (2012) Effect of high temperature on the reproductive development of chickpea genotypes under controlled environments. Funct Plant Biol 39:1009–1018

    Article  Google Scholar 

  • Fang XW, Turner NC, Yan GJ, Li FM, Siddique KHM (2010) Flower numbers, pod production, pollen viability, and pistil function are reduced and flower and pod abortion increased in chickpea (Cicer arietinum L.) under terminal drought. J Exp Bot 61:335–345

    Article  CAS  Google Scholar 

  • FAOSTAT (2019) Accessed 7 Mar 2021

  • Gaur PM, Tripathi S, Gowda CLL, Ranga Rao GV, Sharma HC, Pande S, Sharma M (2010) Chickpea Seed Production Manual. Patancheru 502 324, Andhra Pradesh, India: International Crops Research Institute for the Semi-Arid Tropics

  • Gaur PM, Krishnamurthy L, Kashiwagi J (2008) Improving drought-avoidance root traits in chickpea (Cicer arietinum L.): current status of research at ICRISAT. Plant Prod Sci 11:3–11

    Article  Google Scholar 

  • Gaur PM, Samineni S, Thudi M, Tripathi S, Sajja SB, Jayalakshmi V, Mannur DM, Vijayakumar AG, Ganga Rao NVPR, Ojiewo C, Fikre A, Kimurto P, Kileo RO, Girma N, Chaturvedi SK, Varshney RK, Dixit GP (2019) Integrated breeding approaches for improving drought and heat adaptation in chickpea (Cicer arietinum L.). Plant Breed 138:389–400.

    Article  CAS  Google Scholar 

  • Glaszmann JC, Kilian G, Upadhyaya HD, Varshney RK (2010) Accessing genetic diversity for crop improvement. Curr Opin Plant Biol 13:167–173

    Article  CAS  Google Scholar 

  • IPCC (2018) Summary for policymakers. In: Masson-Delmotte V, Zhai P, Pörtner HO, Roberts D, Skea J, Shukla PR, Pirani A, Moufouma-Okia W, Péan C, Pidcock R, Connors S, Matthews JBR, Chen Y, Zhou X, Gomis MI, Lonnoy E, Maycock T, Tignor M, Waterfield T (eds) Global warming of 1.5°C. An IPCC special report on the impacts of global warming of 1.5°C above pre-industrial levels and related global greenhouse gas emission pathways, in the context of strengthening the global response to the threat of climate change, sustainable development, and efforts to eradicate poverty. World Meteorological Organization, Geneva, p 32

  • Jha UC, Shil S (2015) Association analysis of yield contributing traits of chickpea genotypes under high temperature condition. Trends Biosci I8:2335–2341

    Google Scholar 

  • Jha UC, Basu P, Singh D (2015) Genetic variation and diversity analysis of chickpea genotypes based on quantitative traits under high temperature stress. Int J Bio-Resour Stress Manag 6:700–706

    Article  Google Scholar 

  • Jha UC, Kole PC, Singh NP, Shil S, Gawande D (2017) Genetic variability and association of various heat stress relevantindices for selecting heat-tolerant chickpea (Cicer arietinum L.) genotype. Int J Bio-Resour Stress Manag 8:733–739

    Article  Google Scholar 

  • Krishnamurthy L, Gaur PM, Basu PS, Chaturvedi SK, Tripathi S, Vadez V, Rathore A, Varshney R, Gowda CLL (2011) Large genetic variation for heat tolerance in the reference collection of chickpea (Cicer arietinum L.) germplasm. Plant Genet Resour 9:59–69

    Article  Google Scholar 

  • Leport L, Turner NC, French RJ, Barr MD, Duda R, Daves SL (1999) Physiological responses of chickpea genotypes to terminal drought in a Mediterranean-type environment. Eur J Agron 11:279–291

    Article  Google Scholar 

  • Leport L, Turner N, Davies S, Siddique K (2006) Variation in pod production and abortion among chickpea cultivars under terminal drought. Eur J Agron 24:236–246

    Article  Google Scholar 

  • Munier-Jolain NG, Ney B (1998) Seed growth rate in grain legumes II. Seed growth rate depends on cotyledon cell number. J Exp Bot 49:1971–1976

    Article  CAS  Google Scholar 

  • Pang J, Turner NC, Khan T, Du YL, Xiong JL, Colmer TD (2017) Response of chickpea (Cicer arietinum L.) to terminal drought: leaf stomatal conductance, pod abscisic acid concentration, and seed set. J Exp Bot 68:1973–1985

    CAS  PubMed  Google Scholar 

  • Poland JA, Bradbury PJ, Buckler ES, Nelson RJ (2011) Genome-wide nested association mapping of quantitative resistance to northern leaf blight in maize. Proc Natl Acad Sci 108:6893–6898

    Article  CAS  Google Scholar 

  • Roorkiwal M, Bharadwaj C, Barmukh R, Dixit GP, Thudi M, Gaur PM et al (2020) Integrating genomics for chickpea improvement: achievements and opportunities. Theor Appl Genet 133:1703–1720.

    Article  PubMed  PubMed Central  Google Scholar 

  • Scott MF, Ladejobi O, Amer S, Bentley AR, Biernaskie J, Scott BA, Clark M et al (2020) Multi-parent populations in crops: a toolbox integrating genomics and genetic mapping with breeding. Heredity.

    Article  PubMed  PubMed Central  Google Scholar 

  • Shah TM, Imran M, Atta BM, Ashraf MY, Hameed A, Waqar I (2020) Selection and screening of drought tolerant high yielding chickpea genotypes based on physio-biochemical indices and multi-environmental yield trials. BMC Plant Biol 20:171.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Singh P, Nedumaran S, Boote KJ, Gaur PM, Srinivas K, Bantilana MCS (2014) Climate change impacts and potential benefits of drought and heattolerance in chickpea in South Asia and East Africa. Eur J of Agron 52:123–137

    Article  Google Scholar 

  • Sita K, Sehgal A, HanumanthaRao BNRM, Vara Prasad PV, Kumar S (2017) Food legumes and rising temperatures: effects, adaptive functional mechanisms specific to reproductive growth stage and strategies to improve heat tolerance. Front Plant Sci 8:1–30.

    Article  Google Scholar 

  • Summerfield RJ, Wien HC (1980) Effects of photoperiod and air temperature on growth and yield of economic legumes. In: Summerfield RJ, Bunting AH (eds) Advances in legume science. Her Majesty’s Stationery Office, London, pp 17–36

    Google Scholar 

  • Summerfield RJ, Hadley P, Roberts EH, Minchin FR, Rawsthrone S (1984) Sensitivity of chickpea (Cicer arietinum L.) to hot temperatures during the reproductive period. Exp Agric 20:77–93

    Article  Google Scholar 

  • Upadhyaya H, Dronavalli N, Laxmipathi G, Cholenahalli SS (2011) Identification and evaluation of chickpea germplasm for tolerance to heat stress. Crop Sci 51:2079.

    Article  Google Scholar 

  • Varshney RK, Song C, Saxena RK, Azam S, Yu S, Sharpe AG, Cannon S, Baek J, Rosen BD, Tar’an B, Millan T, Zhang X, Ramsay LD, Iwata A, Wang Y, Nelson W, Farmer AD, Gaur PM, Soderlund C, Penmetsa RV, Xu C, Bharti AK, He W, Winter P, Zhao S, Hane JK, Carrasquilla-Garcia N, Condie JA, Upadhyaya HD, Luo MC, Thudi M, Gowda CLL, Singh NP, Lichtenzveig J, Gali KK, Rubio J, Nadarajan N, Dolezel J, Bansal KC, Xu X, Edwards D, Zhang G, Kahl G, Gil J, Singh KB, Datta SK, Jackson SA, Wang J, Cook DR (2013) Draft genome sequence of chickpea (Cicer arietinum) provides a resource for trait improvement. Nat Biotechnol 31:240–246

    Article  CAS  Google Scholar 

  • Wang J, Gan YT, Clarke F, McDonald CL (2006) Response of chickpea yield to high temperature stress during reproductive development. Crop Sci 46:2171–2178

    Article  Google Scholar 

  • Yadav VK, Neelum Y, Singh RD, Yadav N (1996) Metabolic changes and their impact on yield in chickpea under water stress. Plant Physiol Biochem 23:49–52

    Google Scholar 

  • Yu J, Holland JB, McMullen MD, Buckler ES (2008) Genetic design and statistical power of nested association mapping in maize. Genetics 178:539–551

    Article  Google Scholar 

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Financial support from CGIAR Generation Challenge Program (GCP) and Bill & Melinda Gates Foundation through Tropical Legumes projects is gratefully acknowledged. This work has been undertaken as part of the CGIAR Research Program on Grain Legumes and Dryland Cereals.

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PMG and RKV conceived the idea; PMG and SrS developed the MAGIC lines; SoS, BM and UC evaluated the MAGIC lines and analyzed the data; SrS wrote the initial draft of the MS; and MT, RKV and PMG reviewed and edited the MS. All authors read the manuscript and agreed with its content.

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Correspondence to Pooran M. Gaur.

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Samineni, S., Sajja, S.B., Mondal, B. et al. MAGIC lines in chickpea: development and exploitation of genetic diversity. Euphytica 217, 137 (2021).

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