Abstract
Stripe (yellow) rust caused by Puccinia striiformis f. sp. tritici (Pst) is a major disease in many wheat growing regions. The objective of this study was to characterise stripe rust resistance in a common wheat landrace PI388231, originated from Sindh province of Pakistan, which confers high to moderate levels of resistance against Pst pathotypes prevalent in Pakistan. A recombinant inbred line (RIL) population was developed by crossing PI388231 with the susceptible parent Avocet ‘s’. Screening of this population with Pakistani Pst pathotype 574212 in glasshouse indicated the presence of a single gene and the locus was temporarily named YrPak. Bulked segregant analysis using the targeted genotyping-by-sequencing (tGBS) platform located YrPak on the short arm of chromosome 5B. YrPak-linked SNPs were converted into kompetitive-allele specific PCR (KASP) markers and were genotyped on the parents. Five KASP markers sunKASP_330, sunKASP_334, sunKASP_335, sunKASP_338 and sunKASP_341 that showed clear clustering between parents were tested on the entire RIL population. Markers sunKASP_338 and sunKASP_341 flanked YrPak. The markers sunKASP_338 and sunKASP_341 amplified alleles alternate to those linked with YrPak in 86% and 83% of wheat cultivars tested, respectively. This population segregated for two adult plant resistance genes in addition to YrPak. The presence of pleiotropic locus Yr46/Lr67/Sr55 was confirmed using the gene specific marker TM10_67T and the second gene remains unknown.
Similar content being viewed by others
Availability of data and materials
All data are given in the manuscript.
Code availability
Publicly available softwares are used in this study.
References
Bansal UK, Forrest K, Hayden M, Miah H, Singh D, Bariana HS (2011) Characterization of a new stripe rust resistance gene Yr47 and its genetic association with the leaf rust resistance gene Lr52. Theor Appl Genet 122:1461–1466
Bariana HS (2003) Breeding for disease resistance. In: Thomas B (ed) Encyclopedia of Applied Plant Sciences. Academic Press, Harcourt, pp 244–253
Bariana HS, Bansal UK (2017) Breeding for disease resistance. In: Thomas B, Murray BG, Murphy DJ (Editors in Chief), Encyclopaedia of applied plant sciences. Volume 3, Waltham, MA, Academic Press, pp 69–76
Bariana H, McIntosh R (1995) Genetics of adult plant stripe rust resistance in four Australian wheats and the French cultivar ‘Hybride-de-Bersée.’ Plant Breed 114:485–491
Bariana HS, Bell JA, Brown GN, Standen GE (1996). A catalogue of the national cereal rusts control program (NCRCP) germplasm I: wheat. In: Proceedings of 8th assembly of wheat breeding Society, Canberra, Australia, pp 137–140
Bariana HS, Miah H, Brown GN, Willey N, Lehmensiek A (2007) Molecular mapping of durable rust resistance in wheat and its implication in breeding. In: Buck HT, Nisi JE, Salomón N (eds) Wheat production in stressed environments: Proc 7th Intl Wheat Conference, 27 November–2 December 2005, Mar del Plata, Argentina. Springer, Dordrecht, pp 723–728
Bothmer R, Von Seberg O, Jacobsen N (1992) Genetic resources in the Triticeae. Hereditas 116:141–150
Chhetri M, Bariana H, Kandiah P, Bansal U (2016) A new stripe rust resistance gene and its interaction with Yr46 for enhanced resistance. Phytopathol 106:1530–1534
Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
Dyck PL, Samborski DJ (1979) Adult-plant leaf rust resistance in PI250413, an introduction of common wheat. Can J Plant Sci 59(2):329–332
Hovmoller MS, Walter S, Bayles RA, Hubbard A, Flath K, Sommerfeldt N, Leconte M, Czembor P, Rodriguez-Algaba J, Thach T, Hansen JG, Lassen P, Justesen AF, Aliaf A, De Vallavieille-Pope C (2016) Replacement of the European wheat yellow rust population by new races from the center of diversity in the near-Himalayan region. Plant Pathol 65:402–441
Kosambi D (1943) The estimation of map distances from recombination values. Ann Eugen 12:172–175
Lagudah ES, McFadden H, Singh RP, Huerta-Espino J, Bariana HS, Spielmeyer W (2006) Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor Appl Genet 114:21–30
Lorieux M (2012) MapDisto: fast and efficient computation of genetic linkage maps. Mol Breed 30:1231–1235
McNeal FH, Konzak CF, Smith EP, Tate WS, Russell TS (1971) A uniform system for recording and processing cereal research data. U.S. Department of Agriculture. Agric Res Ser 34–121, 42 pp
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. PNAS 88:9828–9832
Moore JW, Herrera-Foessel S, Lan C, Schnippenkoetter W, Ayliffe M, Huerta-Espino J, Lillemo M, Viccars L, Milne R, Periyannan S, Kong X, Spielmeyer W, Talbot M, Bariana H, Patrick JW, Dodds P, Singh R, Lagudah E (2015) A recently evolved hexose transporter variant confers resistance to multiple pathogens in wheat. Nat Genet 47:1494–1498
Murray GM, Brennan JP (2009) The current and potential costs from diseases of wheat in Australia. Grains Research and Development Corporation, Canberra, p 69
Nsabiyera V, Bariana HS, Qureshi N, Wong D, Forrest KL, Hayden MJ, Bansal UK (2018) Characterization and molecular mapping of adult plant stripe rust resistance in wheat accession Aus27284. Theor Appl Genet 131:1459–1467
Qureshi N, Bariana H, Forrest K, Hayden M, Keller B, Wicker T, Faris J, Salina E, Bansal U (2017) Fine mapping of the chromosome 5B region carrying closely linked rust resistance genes Yr47 and Lr52 in wheat. Theor Appl Genet 130:495–504
Randhawa M, Bansal UK, Valárik M, Klocová B, Doležel J, Bariana HS (2014) Molecular mapping of stripe rust resistance gene Yr51 in chromosome 4AL of wheat. Theor Appl Genet 127:317–324
Randhawa M, Bariana HS, Mago R, Bansal U (2015) Mapping of a new stripe rust resistance locus Yr57 on chromosome 3BS of wheat. Mol Breed 35:1–8
Rosewarne GM, Singh RP, Hererra-Foessel J, Forrest KL, Hayden JM, Rebetzke GJ (2012) Analysis of wheat and stripe rust severities reveals pathotype changes and multiple minor QTLs associated with resistance in an Avocet×Pastor wheat population. Theor Appl Genet 124:1283–1294
Rosewarne G, Herrera-Foessel S, Singh R, Huerta-Espino J, Lan C, He Z (2013) Quantitative trait loci of stripe rust resistance in wheat. Theor Appl Genet 126:2427–2449
Stubbs RW, Prescott JM, Saari EE, Dubin HJ (1986) Cereal disease methodology manual. International Maize and Wheat Improvement Center (CIMMYT), Mexico
The International Wheat Genome Sequencing Consortium (IWGSC). (2018) Shifting the limits in wheat research and breeding using a fully annotated reference genome. Sci 361: eaar7191
Voorrips RE (2002) MapChart: Software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78
Wang S, Wong D, Forrest K, Allen A, Chao S, Huang BE, Maccaferri M, Salvi S, Milner SG, Cattivelli L (2014) Characterization of polyploid wheat genomic diversity using a high density 90,000 single nucleotide polymorphism array. Plant Biotech J 12:787–796
Wellings CR (2007) Puccinia striiformis in Australia: a review of the incursion, evolution, and adaptation of stripe rust in the period 1979–2006. Aust J Agric Res 58:567–575
Wellings CR, McIntosh RA (1990) Puccinia striiformis f.sp. tritici in Australia. Pathogenic changes during the 1st 10 years. Plant Pathol 39:316–325
Acknowledgements
Maryam Tariq is highly thankful to the Higher Education Commission of Pakistan for International Research Support Initiative Program (IRSIP) award and the University of Sydney providing resources for this research. I also acknowledge the staff of Crop Disease Research Institute (CDRI), Murree for supervision and providing research material and resources for this study.
Funding
Higher Education Commission of Pakistan, International Research Support Initiative Program (IRSIP).
Author information
Authors and Affiliations
Contributions
JM planned the study, MT drafted the manuscript; developed segregating population and performed phenotyping, KF and NQ performed genotyping and tGBS analysis, UB and MT designed the KASP primers and MT performed KASP genotyping, UB, HB, KF, NQ, JM and SH edited the manuscript.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Tariq, M., Mirza, J.I., Hussain, S. et al. Molecular mapping of all stage stripe rust resistance gene YrPak in wheat landrace PI388231. Euphytica 217, 121 (2021). https://doi.org/10.1007/s10681-021-02856-2
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s10681-021-02856-2