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Tomato near isogenic lines to unravel the genetic diversity of S. pimpinellifolium LA0722 for fruit quality and shelf life breeding

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Abstract

The introgression of genes from the wild species S. pimpinellifolium has the potential to improve tomato commercial varieties for numerous fruit quality traits. One of particular interest for fresh-market tomatoes is long shelf life (SL). Twenty-two near isogenic lines (NILs) were developed in three to four backcrosses using S. pimpinellifolium accession LA0722 as the donor parent, based on phenotypic selection for long SL and using a set of 28 SSRs for marker assisted selection. A total of 30 QTLs for fruit quality were found and 18 were subsequently validated in previous generations. A two year trial of the NILs homozygous for the LA0722 introgressions showed several novel QTLs. Fruit quality QTLs revealed an increased effect promoted by LA0722 alleles. A SL QTL was found on chromosome 9 and was consistent both years. A wild introgression on chromosome 3 showed a stable QTL for increased firmness and for yellow fruits. Therefore, the wild introgressions provide a source of variation with positive effects in fruit traits of commercial value. While obtaining new varieties, this strategy provided an effective method that integrates the QTL analysis and the prediction of positive alleles in wild germplasm.

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Data supporting the findings of this work are available within this published article and its Electronic Supplementary Material.

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Acknowledgements

This work was supported by Agencia Nacional de Promoción Científica y Tecnológica PICT2015-0424, Consejo Nacional de Investigaciones Científicas y Técnicas PIP008-2015-2017 GI and PUE0043. We thank the Tomato Genetic Resources Center, University of California, Davis, California, USA for kindly providing seeds of the wild accession LA0722 of S. pimpinellifolium and Estación Experimental Agropecuaria - Instituto Nacional de Tecnología Agropecuaria Cerrillos, Salta, Argentina for kindly providing seeds of the Caimanta cultivar. We thank Prof. Trad. Virginia Cattolica for the language editing.

Funding

This work was supported by Agencia Nacional de Promoción Científica y Tecnológica PICT2015-0424, Consejo Nacional de Investigaciones Científicas y Técnicas PIP008-2015-2017 GI and PUE0043.

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Author notes

  1. Di Giacomo and Luciani did equal contribution to the manuscript.

Authors and Affiliations

  1. Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR-CONICET-UNR), Campo Experimental Villarino, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla, S2125ZAA, Santa Fe, Argentina

    Melisa Di Giacomo, Marianela Dana Luciani, Vladimir Cambiaso, Gustavo Rubén Rodríguez & Javier Hernán Pereira da Costa

  2. Consejo de Investigaciones de la Universidad Nacional de Rosario CIUNR, Santa Fe, Argentina

    Roxana Zorzoli

  3. Cátedra de Genética, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla, S2125ZAA, Santa Fe, Argentina

    Vladimir Cambiaso, Roxana Zorzoli, Gustavo Rubén Rodríguez & Javier Hernán Pereira da Costa

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  1. Melisa Di Giacomo
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  2. Marianela Dana Luciani
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  3. Vladimir Cambiaso
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  6. Javier Hernán Pereira da Costa
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Contributions

Melisa Di Giacomo and Marianela D. Luciani: first draft of the manuscript, plant material development, molecular and phenotype characterization, formal analysis; Vladimir Cambiaso: review and editing, molecular and phenotype characterization; Roxana Zorzoli: review and editing, conception and design, formal analysis; Gustavo R. Rodríguez: review and editing, conception and design, formal analysis, funding acquisition; Javier H. Pereira da Costa: review and editing, plant material development, conception and design, formal analysis, funding acquisition.

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Correspondence to Javier Hernán Pereira da Costa.

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Di Giacomo, M., Luciani, M.D., Cambiaso, V. et al. Tomato near isogenic lines to unravel the genetic diversity of S. pimpinellifolium LA0722 for fruit quality and shelf life breeding. Euphytica 216, 126 (2020). https://doi.org/10.1007/s10681-020-02649-z

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