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QTL analysis of main agronomic traits in rice under low temperature stress

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To identify cold tolerance at the reproductive stage in rice (Oryza sativa L.), the cold susceptible accession, IAPAR-9 (tropical japonica) was crossed with the cold tolerant accessions Akihikari and Liaoyan241 (temperate japonica) to construct two recombinant inbred lines (RIL) to detected QTLs related to cold-tolerance of rice through analyze phenotypic variation. The two RILs and their parents were experimentally grown in natural conditions and cold water irrigation at one site, Gongzhuling Jilin (China), and at natural low temperature in another site, Kunming Yunnan (China). We measured plant height, panicle length, number of panicles per plant, grain number per panicle, seed setting rate and panicle exsertion of parents and RIL populations in the maturation stage. In the three treatment environments, the traits of the two RILs showed normal distributions, and both exhibited transgressive segregation. Low temperature stress had greater influence on number of panicles per plant and grain number per panicle and lower influence on plant height and panicle length. Seventeen quantitative trait locus (QTLs) were detected, distributed on chromosomes 1, 3-6, 8, 11 and 12. Among these, four QTLs had values of phenotypic variance explained that were greater than 10%. There were two QTLs, qPN12-2E2 and qPN12-2E3, detected in the IAPAR-9/Akihikari RIL population in same region under cold water irrigation condition in Gongzhuling Jilin and natural low temperature in Kunming Yunnan. Major regions related to cold tolerance QTLs in these two populations was on chromosome 12 from RM511 to RM3739. The cold tolerance allele was from the Akihikari and Liaoyan241 in the respective populations for both QTL regions.

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Acknowledgements

This work was supported by the National Key Research and Development Program of China (2016YFD0100101, 2016YFD0100301), the National Natural Science Fundation of China (31671664), the National Science and Technology Support Program of China (2015BAD01B01-1), CAAS Science and Technology Innovation Program, National Infrastructure for Crop Germplasm Resources (NICGR2017-01), Protective Program of Crop Germplasm of China (2017NWB036-01, 2017NWB036-12-2).

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Authors and Affiliations

  1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Jianghong Tang, Xiaoding Ma, Di Cui, Bing Han, Leiyue Geng & Longzhi Han

  2. Chongqing Engineering Research Center of Specialty Crop Resource, Chongqing Normal University, Chongqing, 401331, China

    Jianghong Tang & Zhengwu Zhao

  3. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Yafei Li

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  1. Jianghong Tang
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Correspondence to Zhengwu Zhao, Yafei Li or Longzhi Han.

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Tang, J., Ma, X., Cui, D. et al. QTL analysis of main agronomic traits in rice under low temperature stress. Euphytica 215, 193 (2019). https://doi.org/10.1007/s10681-019-2507-1

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