Abstract
To develop a gene-based DNA selection marker for the purple and red colored root characteristics, multiplex-PCR was performed for genotyping of Raphanus sativus flavonoid 3′ hydroxylase (RsF3′H) in several cultivars and progenies. In cultivars, DNA with an approximately 450-bp in size was amplified from several radishes, such as purple-, white-, green-, and black-root radishes, whereas approximately 750-bp in size was amplified from red-root radishes. In progenies from red- and white-roots, almost F1 progenies had purple-root with DNA fragments of approximately 450- and 750-bp. In F2 progenies, two band patterns which are 450-bp, and both 450- and 750-bp fragments were amplified from purple-root individuals, whereas only 750-bp fragment were amplified from red-root individuals. In contrast, three band patterns were amplified from white-root individuals. Therefore, our data indicated that mutant RsF3′H allele-based marker could select the potential red root genotype effectively in breeding program of purple and red root radishes.
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Acknowledgements
This study was supported by Grant-in-Aid for Scientific Research (KAKENHI No. 16K07597) from Japan Society for the Promotion of Science (JSPS).
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Masukawa, T., Cheon, KS., Mizuta, D. et al. Development of mutant RsF3′H allele-based marker for selection of purple and red root in radish (Raphanus sativus L. var. longipinnatus L. H. Bailey). Euphytica 215, 119 (2019). https://doi.org/10.1007/s10681-019-2442-1
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DOI: https://doi.org/10.1007/s10681-019-2442-1