Abstract
Three genes for resistance to Erysiphe pisi, named er1, er2 and Er3 have been described in pea so far. er1 gene is located in pea linkage group VI, while er2 gene has been mapped in LGIII. SCAR and RAPD markers tightly linked to Er3 gene have been identified, but the position of these markers in the pea genetic map was unknown. The objective of this study was to localize Er3 gene in the pea genetic map. Towards this aim, the susceptible pea cv. Messire (er3er3) and a resistant near isogenic line of Messire (cv. Eritreo, Er3Er3) were surveyed with SSRs with known position in the pea map. Three SSRs were polymorphic between “Messire” and “Eritreo” and further surveyed in two contrasting bulks formed by homozygous Er3Er3/er3er3 individuals obtained from a F2 population derived from the cross C2 (Er3Er3) × Messire (er3er3). A single marker, AA349, was polymorphic between the bulks. Subsequently, other ten markers located in the surrounding of AA349 were selected and analysed in Er3Er3 and er3er3 plants. As a results, another SSR, AD61, was found to be polymorphic between Er3Er3 and er3er3 plants. Further linkage analysis confirmed that SSRs AA349 and AD61 were linked to Er3 and to the RAPD and SCAR markers previously reported to be linked to this gene. Er3 gene was located in pea LGIV at 0.39 cM downstream of marker AD61. The location of Er3 gene in the pea map is a first step toward the identification of this gene.
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References
Attanayake RN, Glawe DA, McPhee KE, Dugan FM, Chen W (2010) Erysiphe trifolii—a newly recognized powdery mildew pathogen of pea. Plant Pathol 59:712–720
Carrillo E, Satovic Z, Aubert G, Boucherot K, Rubiales D, Fondevilla S (2014) Identification of quantitative trait loci and candidate genes for specific cellular resistance responses against Didymella pinodes in pea. Plant Cell Rep 33:1133–1145
Fondevilla S, Carver TLW, Moreno MT, Rubiales D (2006) Macroscopic and histological characterisation of genes er1 and er2 for powdery mildew resistance in pea. Eur J Plant Pathol 115:309–321
Fondevilla S, Carver TLW, Moreno MT, Rubiales D (2007) Identification and characterisation of sources of resistance to Erysiphe pisi Syd. in Pisum spp. Plant Breed 126:113–119. https://doi.org/10.1111/j.1439-0523.2006.01312.x
Fondevilla S, Rubiales D, Moreno MT, Torres AM (2008) Identification and validation of RAPD and SCAR markers linked to the gene Er3 conferring resistance to Erysiphe pisi DC in pea. Mol Breed 22:193–200. https://doi.org/10.1007/s11032-008-9166-6
Fondevilla S, Cubero JI, Rubiales D (2010) Confirmation that the Er3 gene, conferring resistance to Erysiphe pisi in pea, is a different gene from er1 and er2 genes. Plant Breed 130:281–282
Fondevilla S, Chattopadhyay C, Khare N, Rubiales D (2013) Erysiphe trifolii is able to overcome er1 and Er3 resistance genes but not er2. Eur J Plant Pathol 136:557–563
Gritton ET, Ebert RD (1975) Interaction of planting date and powdery mildew on pea plant performance. J Am Soc Hortic Sci 100:137–142
Harland SC (1948) Inheritance of immunity to mildew in Peruvian forms of Pisum sativum. Heredity 2:263–269
Heringa RJ, Van Norel A, Tazelaar MF (1969) Resistance to powdery mildew (Erysiphe polygoni D.C.) in peas (Pisum sativum L.). Euphytica 18:163–169
Humphry M, Reinstädler A, Ivanov S, Bisseling T, Panstruga R (2011) Durable broadspectrum powdery mildew resistance in pea er1 plants is conferred by natural loss-of-function mutations in PsMLO1. Mol Plant Pathol 12:866–878
Katoch V, Sharma S, Pathania S, Banayal DK, Sharma SK, Rathour R (2010) Molecular mapping of pea powdery mildew resistance gene er2 to pea linkage group III. Mol Breed 25:229–237
Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Loridon K, McPhee K, Morin J, Dubreuil P, Pilet-Nayel ML, Aubert G, Rameau C, Baranger A, Coyne C, Lejeune- Henaut I, Burstin J (2005) Microsatellite marker polymorphism and mapping in pea (Pisum sativum L.). Theor Appl Genet 111:1022–1031
Ondřej M, Dostálová R, Odstrčilová L (2005) Response of Pisum sativum germplasm resistant to Erysiphe pisi to inoculation with Erysiphe baeumleri, a new pathogen of peas. Plant Prot Sci 41:95–103
Ram B, Prasad CS (1994) Assessment of losses by powdery mildew, Erysiphe polygoni (DC) on pea. Natl Acad Sci Lett 17:175–177
Smith PH, Foster EM, Boyd LA, Brown JKM (1996) The early development of Erysiphe pisi on Pisum sativum L. Plant Pathol 45:302–309
Tayed N, Aubert G, Pilet-Nayel ML, Lejeune-Hénaut I, Warkentin TD, Bursting J (2015) Genomic tools in pea breeding programs: status and perspectives. Front Plant Sci 6:1037
Timmerman GM, Frew TJ, Weeden NF (1994) Linkage analysis of er1, a recessive Pisum sativum gene for resistance to powdery mildew fungus (Erysiphe pisi DC). Theor Appl Genet 88:1050–1055
Tiwari KR, Penner GA, Warkentin TD (1999) Identification of AFLP markers for powdery mildew resistance gene er2 in pea. Pisum Genet 31:27–29
Warkentin TD, Rashid KY, Xue AG (1996) Fungicidal control of powdery mildew in field pea. Can J Plant Sci 76:933–935
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This research was supported by Projects AGL2014-52871-R and AGL2017-82907-R co-financed by FEDER.
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Cobos, M.J., Satovic, Z., Rubiales, D. et al. Er3 gene, conferring resistance to powdery mildew in pea, is located in pea LGIV. Euphytica 214, 203 (2018). https://doi.org/10.1007/s10681-018-2292-2
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DOI: https://doi.org/10.1007/s10681-018-2292-2