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Characterization of an EMS-induced soybean mutant with an increased content of Af saponin and a new component Ab-δ in the seed hypocotyl

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Abstract

Saponins are one of the components present in the soybean seeds that have various functional properties. The chemical structures and concentration of soyasaponins affect the taste of the processed soyfood, thereby limiting its industrial applications. Therefore, it is important to understand saponin biosynthesis to explore natural and artificial variation in the saponin components, which can be modified to suit its application. The objective of the present study was to identify and characterize an EMS-induced soybean mutant with an altered saponin composition from a pool of 892 M4 lines. The mutant PE1905 showed an increased content of saponin Af (336.0%). The content of saponin Ab, DDMP-αg, and DDMP-βg was decreased in the mutant PE1905 by 89.3, 24.8, and 63.1%, respectively compared to the wild-type Pungsannamul. Additionally, four new components were detected in the mutant PE1905 that were absent in the wild type. Of these, the compound 4 (designated as Ab-δ) had the highest concentration, and therefore it was further characterized by HPLC and LC-PDA/MS/MS analysis to know the chemical structure, and molecular weight and formula. Considering these details, along with the alterations in the saponin Af and Ab concentrations, it was presumed that the Ab-δ acts as a precursor for the synthesis of saponin Af and Ab. Thus, we predicted a biosynthetic pathway from the Ab-δ to Ab saponin. The inheritance analysis showed that the concentration of saponin Ab-δ is controlled by a single recessive gene in the mutant PE1905. The results from the present study would be helpful in understanding the mechanisms behind altered seed saponin composition in soybeans.

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Author notes

  1. Cheol Woo Park

    Present address: Division of Agrobiology, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido, 060-0808, Japan

  2. Krishnanand P. Kulkarni

    Present address: Department of Plant and Soil Sciences, Institute for Agricultural Biosciences, Oklahoma State University, Ardmore, OK, 73401, USA

  3. Cheol Woo Park and Krishnanand P. Kulkarni have contributed equally to this work.

Authors and Affiliations

  1. School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea

    Cheol Woo Park, Krishnanand P. Kulkarni, Minsu Kim, Jong Tae Song & Jeong-Dong Lee

  2. Department of Applied Biological Chemistry, Iwate University, Morioka, Iwate, 020-88250, Japan

    Kyosuke Mukaiyama & Chigen Tsukamoto

  3. Department of Biotechnology, Chonnam National University, Yeosu, Chonnam, 550-749, Republic of Korea

    Gyuhwa Chung

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  1. Cheol Woo Park
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Correspondence to Jeong-Dong Lee.

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Park, C.W., Kulkarni, K.P., Kim, M. et al. Characterization of an EMS-induced soybean mutant with an increased content of Af saponin and a new component Ab-δ in the seed hypocotyl. Euphytica 214, 163 (2018). https://doi.org/10.1007/s10681-018-2242-z

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