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Marker-assisted backcrossing of lcyE for enhancement of proA in sweet corn

Abstract

Vitamin A deficiency (VAD) compromises the immune system and is the leading cause of preventable blindness in children in many developing countries. Biofortification provides a sustainable way to prevent VAD and other micronutrient malnutrition problems. Sweet corn is one of the most popular vegetables and is an important provitamin A (proA) resource for humans. In this study, one common corn line with favorable lcyE alleles was used as donor parental line, and four elite sweet corn lines were employed as recipient lines. The favorable lcyE allele was introgressed into four sweet corn lines by marker-assisted selection (MAS). The functional marker 3′indel was selected as foreground marker, and 196 simple sequence repeats were utilized as background selection markers. Background selection was performed in each backcrossed population, and individuals with higher recovery ratio were retained. Twelve agronomic traits were investigated in the BC3F3 populations, which did not show any significant difference between the converted BC3F3 families and their recurrent parental lines. Further investigation showed that the favorable lcyE allele significantly improves β-carotene, proA, and total carotenoid contents as well as significantly increases α-carotene levels. The average increase in β-carotene, proA, and total carotenoid content was 1.54, 1.85, and 6.77 μg/g, respectively. The β-carotene/total carotenoid ratio increased among three converted lines, except for the K185-converted lines. The findings of the present study indicate that the introgression of the favorable lcyE allele by MAS efficiently increases proA carotenoid content in sweet corn.

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References

  • Azmach G, Menkir A, Spillane C, Gedil M (2018) Genetic loci controlling carotenoid biosynthesis in diverse tropical maize lines. G3 (Bethesda) 8:1049–1065

    Google Scholar 

  • Babu R, Nair SK, Prasanna BM, Gupta HS (2004) Integrating marker-assisted selection in crop breeding—prospects and challenges. Curr Sci 87:607–619

    CAS  Google Scholar 

  • Babu R, Rojas N, Gao S, Yan J, Pixley K (2013) Validation of the effects of molecular marker polymorphisms in LcyE and CrtRB1 on provitamin A concentrations for 26 tropical maize populations. Theor Appl Genet 126:389–399

    Article  CAS  PubMed  Google Scholar 

  • Buckner B, Kelson TL, Robertson DS (1990) Cloning of the y1 Locus of Maize, a Gene Involved in the Biosynthesis of Carotenoids. Plant Cell 2:867–876

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Chander S, Meng Y, Zhang Y, Yan J, Li J (2008) Comparison of nutritional traits variability in selected eighty-seven inbreds from Chinese maize (Zea mays L.) germplasm. J Agric Food Chem 56:6506–6511

    Article  CAS  PubMed  Google Scholar 

  • Dowling JE, Wald G (1961) The role of vitamin A acid. Vitam Horm 18:515–541

    Article  Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Feng F, Wang G, Wang Q, Yang R, Li X (2014) Variation of provitamin A and vitamin E components at milk stage of kernel development in sweet corn. ACTA Agron Sin 40:1227–1234

    Article  CAS  Google Scholar 

  • Gai J (2006) Various theories of crop breeding. China Agricultural Press, Beijing

    Google Scholar 

  • Goffman FD, Boehme T (2001) Relationship between fatty acid profile and vitamin E content in maize hybrids (Zea mays L.). J Agric Food Chem 49:4990–4994

    Article  CAS  PubMed  Google Scholar 

  • Grams GW, Blessin CW, Inglett GE (1970) Distribution of tocopherols within the corn kernel. J Am Oil Chem Soc 47:337–339

    Article  CAS  PubMed  Google Scholar 

  • Harjes CE, Rocheford TR, Bai L, Brutnell TP, Kandianis CB, Sowinski SG, Stapleton AE, Vallabhaneni R, Williams M, Wurtzel ET, Yan J, Buckler ES (2008) Natural genetic variation in lycopene epsilon cyclase tapped for maize biofortification. Science 319:330–333

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • He S, Wan Z, Luo J, Hu J, Yin Y, Qin L (2013) Development situation and countermeasure on Guangdong sweet corn industry in 2012. Guangdong Agric Sci 12:3–5 (in China)

    Google Scholar 

  • Hospital F, Chevalet C, Mulsant P (1992) Using markers in gene introgression breeding programs. Genetics 132:1199–1210

    CAS  PubMed  PubMed Central  Google Scholar 

  • Kurilich AC, Juvik JA (1999) Quantification of carotenoid and tocopherol antioxidants in Zea mays. J Agric Food Chem 47:1948–1955

    Article  CAS  PubMed  Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newberg LA (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  CAS  PubMed  Google Scholar 

  • Li ZH, Matthews PD, Burr B, Wurtzel ET (1996) Cloning and characterization of a maize cDNA encoding phytoene desaturase, an enzyme of the carotenoid biosynthetic pathway. Plant Mol Biol 30:269–279

    Article  CAS  PubMed  Google Scholar 

  • Li Q, Farre G, Naqvi S, Breitenbach J, Sanahuja G, Bai C, Sandmann G, Capell T, Christou P, Zhu C (2010) Cloning and functional characterization of the maize carotenoid isomerase and beta-carotene hydroxylase genes and their regulation during endosperm maturation. Transgenic Res 19:1053–1068

    Article  CAS  PubMed  Google Scholar 

  • Lu H, Romero-Severson J, Bernardo R (2002) Chromosomal regions associated with segregation distortion in maize. Theor Appl Genet 105:622–628

    Article  CAS  PubMed  Google Scholar 

  • Matthews PD, Luo R, Wurtzel ET (2003) Maize phytoene desaturase and zeta-carotene desaturase catalyse a poly-Z desaturation pathway: implications for genetic engineering of carotenoid content among cereal crops. J Exp Bot 54:2215–2230

    Article  CAS  Google Scholar 

  • Ribaut JM, Jiang C, Hoisington D (2002) Simulation experiments on efficiencies of gene introgression by backcrossing. Crop Sci 42:557–565

    Article  Google Scholar 

  • Sandmann G (2001) Genetic manipulation of carotenoid biosynthesis: strategies, problems and achievements. Trends Plant Sci 6:14–17

    Article  CAS  PubMed  Google Scholar 

  • Sun Z, Hans J, Walter MH, Matusova R, Beekwilder J, Verstappen FWA, Ming Z, Echtelt EV, Strack D, Bisseling T (2008) Cloning and characterisation of a maize carotenoid cleavage dioxygenase (ZmCCD1) and its involvement in the biosynthesis of apocarotenoids with various roles in mutualistic and parasitic interactions. Planta 228:789

    Article  CAS  PubMed  Google Scholar 

  • Vallabhaneni R, Wurtzel ET (2009) Timing and biosynthetic potential for carotenoid accumulation in genetically diverse germplasm of maize. Plant Physiol 150:562–572

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wong JC, Lambert RJ, Wurtzel ET, Rocheford TR (2004) QTL and candidate genes phytoene synthase and ζ-carotene desaturase associated with the accumulation of carotenoids in maize. Theor Appl Genet 108:349–359

    Article  CAS  PubMed  Google Scholar 

  • Xia J, Zheng Y (2002) Molecular marker-assisted backcross breeding of maize Rf 3 NIL and its efficient analysis. ACTA Agron Sin 28:339–344

    Google Scholar 

  • Yan J, Kandianis CB, Harjes CE, Bai L, Kim E, Yang X, Skinner DJ, Fu Z, Mitchell S, Li Q, Fernandez MGS, Zaharieva M, Babu R, Fu Y, Palacios N, Li J, DellaPenna D, Brutnell T, Buckler ES, Warburton ML, Rocheford T (2010) Rare genetic variation at Zea mays crtRB1 increases β-carotene in maize grain. Nat Genet 42:322–327

    Article  CAS  PubMed  Google Scholar 

  • Zhang YZ (2007) The funtion and application of vitamin A. Chin Food Nutr 12:55–56 (in China)

    Google Scholar 

Download references

Acknowledgements

The Guangdong Provincial Science and Technology Plan Project and Guangzhou City Science and Technology Plan Project (Project Nos. 2015A020209113, 2015A030313415, 2017B090907023, and 201607010248) supported this study. The National Maize Improvement Center of China, China Agricultural University is gratefully acknowledged. We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

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Correspondence to Faqiang Feng.

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Yang, R., Yan, Z., Wang, Q. et al. Marker-assisted backcrossing of lcyE for enhancement of proA in sweet corn. Euphytica 214, 130 (2018). https://doi.org/10.1007/s10681-018-2212-5

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  • DOI: https://doi.org/10.1007/s10681-018-2212-5

Keywords

  • Sweet corn
  • lcyE
  • Carotenoids
  • Marker-assisted selection