Skip to main content

Advertisement

SpringerLink
Log in

Antixenosis and antibiosis mechanisms of resistance to pod borer, Helicoverpa armigera in wild relatives of chickpea, Cicer arietinum

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

The noctuid pod borer, Helicoverpa armigera is one of the most damaging pests of chickpea, Cicer arietinum. The levels of resistance to H. armigera in the cultivated chickpea are low to moderate, but the wild relatives of chickpea have exhibited high levels of resistance to this pest. To develop insect-resistant cultivars with durable resistance, it is important to understand the contribution of different components of resistance, and therefore, we studied antixenosis and antibiosis mechanisms of resistance to H. armigera in a diverse array of wild relatives of chickpea. The genotypes IG 70012, PI 599046, IG 70022, PI 599066, IG 70006, IG 70018 (C. bijugum), ICC 506EB, ICCL 86111 (cultivated chickpea), IG 72933, IG 72953 (C. reticulatum), IG 69979 (C. cuneatum) and IG 599076 (C. chrossanicum) exhibited non preference for oviposition by the females of H. armigera under multi-choice, dual-choice and no-choice cage conditions. Based on detached leaf assay, the genotypes IG 70012, IG 70022, IG 70018, IG 70006, PI 599046, PI 599066 (C. bijugum), IG 69979 (C. cuneatum), PI 568217, PI 599077 (C. judaicum) and ICCW 17148 (C. microphyllum) suffered significantly lower leaf damage, and lower larval weights indicating high levels of antibiosis than on the cultivated chickpea. Glandular and non-glandular trichomes showed negative correlation with oviposition, while the glandular trichomes showed a significant and negative correlation with leaf damage rating. Density of non-glandular trichomes was negatively correlated with larval survival. High performance liquid chromatography (HPLC) fingerprints of leaf surface exudates showed a negative correlation of oxalic acid with oviposition, but positive correlation with malic acid. Both oxalic acid and malic acid showed a significant negative correlation with larval survival. The wild relatives exhibiting low preference for oviposition and high levels of antibiosis can be used as sources of resistance to increase the levels and diversify the basis of resistance to H. armigera in cultivated chickpea.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Babu GC, Sharma HC, Madhumati T, Raghavaiah G, Murthy KVMK, Rao VS (2014) A semi-synthetic chickpea flour based diet for long-term maintenance of laboratory culture of Helicoverpa armigera. Indian J Entomol 76:336–340

    Google Scholar 

  • Bernays EA, Champman RF (1994) Host plant selection by phytophagopus insects. Chapman & Hall, New York

    Book  Google Scholar 

  • Chen W, Sharma HC, Muehlbauer F (2011) Chickpea and lentil crop protection compendium. American Phytopathological Society, St Paul

    Google Scholar 

  • Cowgill SE, Lateef SS (1996) Identification of antibiotic and antixenotic resistance to Helicoverpa armigera (Lepidoptera: Noctuidae) in chickpea. J Econ Entomol 89:224–229

    Article  Google Scholar 

  • FAO STAT (2014) Food and Agriculture Organisation of the United Nations 2002. IOP Publishing FAO. http://apps.fao.org

  • Girija SPM, Patil SA, Gowda CLL, Sharma HC (2008) Biophysical and biochemical basis of host plant resistance to pod borer, Helicoverpa armigera (Hubner) in chickpea (Cicer arietinum L.). Indian J Genet Plant Breed 68:320–323

    Google Scholar 

  • Green PWC, Stevenson PC, Simmonds MSJ, Sharma HC (2002) Can larvae of the pod borer, Helicoverpa armigera (Lepidoptera: Noctuidae), select between wild and cultivated pigeonpea Cajanus sp. (Fabaceae)? Bull Entomol Res 92:45–51

    PubMed  Google Scholar 

  • Hajjar R, Hodgkin T (2007) The use of wild relatives in crop improvement: a survey of developments over the last 20 years. Euphytica 156:1–13

    Article  Google Scholar 

  • Hossain MA, Haque MA, Prodhan MZH (2008) Effect of pods characteristics on pod borer, Helicoverpa armigera (Hubner), investigation in chickpea. SAARC J Agric 6(1):1–11

    Google Scholar 

  • Jackai LEN, Oghiakhe S (1989) Pod wall trichomes and role in the resistance of two wild cowpea, Vigna vexillata accessions to Maruca testulalis (Geyer), (Lepidoptera: Pyralidae) and Clavigralla tomentosicollois, (Hemiptera: Coreidae). Bull Entomol Res 79:595–605

    Article  Google Scholar 

  • Kranthi KR, Jadhav DR, Kranthi S, Wanjari RR, Ali SS, Russel DA (2002) Insecticide resistance in five major insect pests of cotton in India. Crop Prot 21:449–460

    Article  CAS  Google Scholar 

  • Kulkarni US, Gawande RB, Kulkarni SS, Yadgirwar PV (2004) Comparative studies on the biology of Helicoverpa armigera on different food substrates. J Soils Crops 14:207–208

    Google Scholar 

  • Kumari DA, Reddy DJ, Sharma HC (2006) Antixenosis mechanism of resistance in pigeonpea to the pod borer, Helicoverpa armigera. J Appl Entomol 130(1):10–14

    Article  Google Scholar 

  • Maiti RK, Bidinger FR (1979) Simple approaches to the identification of shoot fly tolerance in sorghum. Indian J Plant Prot 7:135–140

    Google Scholar 

  • Narayanamma VL, Sharma HC, Vijay PM, Gowda CLL, Sriramulu M (2013) Expression of resistance to the pod borer Helicoverpa armigera (Lepidoptera: Noctuidae), in relation to high-performance liquid chromatography fingerprints of leaf exudates of chickpea. Int J Trop Insect Sci 33(4):276–282

    Article  Google Scholar 

  • Navasero RC, Ramaswamy SB (1991) Morphology of leaf surface trichomes and its influence on egglaying by Heliothis virescens. Crop Sci 31:342–353

    Article  Google Scholar 

  • Nimbalkar RK, Shinde SS, Tawar DS, Muley SP (2009) Response of cotton bollworm Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) to different insecticides in Maharashtra, India. World J Agric Sci 5(2):250–255

    CAS  Google Scholar 

  • Peter AJ, Shanower TG (1998) Plant glandular trichomes: chemical factories with many potential uses. Resonance 3:41–45

    Article  Google Scholar 

  • Peter AJ, Shanower TG, Romeis J (1995) The role of plant trichomes in insect resistance: a selective review. Phytophaga 7:41–64

    Google Scholar 

  • Pundir RPS, Mangesha MH (1995) Cross compatibility between chickpea and its wild relative Cicer echinospermum Davis. Euphytica 83:241–245

    Article  Google Scholar 

  • Rembold H (1981) Malic acid in chickpea exudates a marker for Heliothis resistance. Int Chickpea Newsl 4:18–19

    Google Scholar 

  • Romeis J, Shanower TG, Peter AJ (1999) Trichomes on pigeonpea (Cajanus cajan) and two wild Cajanus spp. Crop Sci 39:564–569

    Article  Google Scholar 

  • Ruan YM, Wu KJ (2001) Performances of the cotton bollworm, Helicoverpa armigera on different food plants. Acta Entomol 44:205–212

    Google Scholar 

  • Sarode SV (1999) Sustainable management of Helicoverpa armigera (Hubner). Pestology 13(2):279–284

    Google Scholar 

  • Sarwar M, Ahmad N, Toufiq M (2009) Host plant resistance relationships in chickpea (Cicer arietinum L.) against gram pod borer (Helicoverpa armigera Hubner). Pak J Bot 41(6):3047–3052

    Google Scholar 

  • Shabbir MZ, Arshad M, Hussin B, Nadeen I, Ali S, Abbasi A, Ali Q (2014) Genotypic response of chickpea (Cicer arietinum L.) for resistance against gram pod borer (Helicoverpa armigera). Adv Life Sci 2(1):23–30

    Google Scholar 

  • Shahzad K, Iqbal A, Khalil SK, Khattak S (2005) Response of different chickpea (Cicer arietinum) genotypes to the infestation of pod borer (Helicoverpa armigera) with relation to trichomes. Res J Agric Biol Sci 1(1):120–124

    Google Scholar 

  • Sharma HC, Pampapathy G, Lanka SK, Ridsdill-Smith TJ (2004) Evaluation of wild relatives of chickpea (Cicer spp.) for resistance to pod borer, Helicoverpa armigera (Hubner). Indian J Plant Genet Resour 17(1): 17–26

    Google Scholar 

  • Sharma HC, Ahmad R, Ujagir R, Yadav RP, Singh R, Ridsdill-Smith TJ (2005a) Host plant resistance to cotton bollworm/legume pod borer, Heliothis/Helicoverpa. In: Sharma HC (ed) Heliothis/Helicoverpa management: emerging trends and strategies for future research. Oxford and IBH Publishing, New Delhi, pp 167–208

    Chapter  Google Scholar 

  • Sharma HC, Pampapathy G, Lanka SK, Ridsdill-Smith TJ (2005b) Exploitation of wild Cicer reticulatum germplasm for resistance to Helicoverpa armigera. J Econ Entomol 98(6):2246–2253

    Article  PubMed  CAS  Google Scholar 

  • Sharma HC, Pampapathy G, Lanka SK, Ridsdill-Smith TJ (2005c) Antibiosis mechanism of resistant to pod borer, Helicoverpa armigera in wild relatives of chickpea. Euphytica 142:107–117

    Article  Google Scholar 

  • Sharma HC, Bhagawat MP, Pampapathy G, Sharma JP, Ridsdill-Smith TJ (2006) Perennial wild relatives of chickpea as potential sources of resistance to Helicoverpa armigera. Genet Resour Crop Evol 53:131–138

    Article  Google Scholar 

  • Simmonds MSJ, Stevenson PC (2001) Effects of isoflavonoid from Cicer arietinum on larvae of Helicoverpa armigera. J Chem Ecol 27(5):965–977

    Article  PubMed  CAS  Google Scholar 

  • Srivastava CP, Srivastava RP (1989) Screening for resistance to gram podborer, Heliothis armigera (Hubner), in chickpea (Cicer arientium L.) genotypes and observations on its mechanism of resistance in India. Insect Sci Appl 10(3):255–258

    Google Scholar 

  • Suzana CS, Damiani R, Fortuna LS, Salvadori JR (2015) Desempenho de larvas de Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) em diferentes fontes alimentares. Pesquisa Agropecu Trop 43:480–485

    Article  Google Scholar 

  • Udayagiri S, Mason CE (1995) Host plant constituents as oviposition stimulants for a generalist herbivore: European corn borer. Entomol Exp Appl 76:59–65

    Article  Google Scholar 

  • Van der Maesen LJG, Maxted N, Javadi F, Coles S, Davies AMR (2007) Taxonomy of the genus Cicer revisited. In: Yadav SS, Redden RJ, Chen W, Sharma B (eds) Chickpea breeding and management. CAB International, Wallingford, pp 14–46

    Chapter  Google Scholar 

  • Yoshida M, Cowgill SE, Wightman JA (1995) Mechanisms of resistance to Helicoverpa armigera (Lepidoptera: Noctuidae) in chickpea—role of oxalic acid in leaf exudates as an antibiotic factor. J Econ Entomol 88:1783–1786

    Article  CAS  Google Scholar 

  • Yoshida M, Cowgill SE, Wightman JA (1997) Roles of oxalic and malic acids in chickpea trichome exudate in host-plant resistance to Helicoverpa armigera. J Chem Ecol 23(4):1195–1210

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank the entomology staff at ICRISAT, Patancheru, for their support in carrying out these experiments. The financial support provided by Department of Science and Technology (DST), New Delhi, India, under INSPIRE Fellowship scheme to Mr. Siva Kumar is gratefully acknowledged.

Funding

This study was not funded by any organization.

Author information

Authors and Affiliations

  1. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Telangana, 502324, India

    Siva Kumar Golla, Suraj Prashad Sharma & H. C. Sharma

  2. Acharya N.G. Ranga Agricultural University, Guntur, Andhra Pradesh, 522509, India

    Siva Kumar Golla, P. Rajasekhar & K. V. Hari Prasad

  3. Dr YS Parmar University of Horticulture and Forestry, Nauni, Solan, 173230, Himachal Pradesh, India

    H. C. Sharma

Authors
  1. Siva Kumar Golla
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Rajasekhar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Suraj Prashad Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. V. Hari Prasad
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H. C. Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

HC, PR and SK conceived and designed the research. SK conducted the experiments, analyzed the results and wrote the paper. SP and HP helped in data analysis and contributed in preparing manuscript. All the authors read and approved the manuscript.

Corresponding author

Correspondence to H. C. Sharma.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

This is not applicable as this article does not contain any studies with human participants performed by any of the authors.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Golla, S.K., Rajasekhar, P., Sharma, S.P. et al. Antixenosis and antibiosis mechanisms of resistance to pod borer, Helicoverpa armigera in wild relatives of chickpea, Cicer arietinum. Euphytica 214, 88 (2018). https://doi.org/10.1007/s10681-018-2168-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s10681-018-2168-5

Keywords

Search

Navigation