Adult plant leaf rust resistance derived from the wheat landrace cultivar Americano 44d is conditioned by interaction of three QTL
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Americano 44 is a landrace derived wheat cultivar from Uruguay that has had long lasting resistance to the leaf rust pathogen Puccinia triticina. A population of 92 recombinant inbred lines derived from the cross ‘Thatcher*3/Americano 44d’ was evaluated for segregation of adult plant leaf rust resistance in four field plot tests and two greenhouse tests. A genetic map was constructed with 381 Diversity Array Technology markers, five simple sequence repeat markers, and seven kompetitive allele specific PCR markers. Significant effects for reduction of leaf rust severity were found for quantitative trait loci (QTL) on chromosomes 3AS, 3DS and 6DS. Individually the 3AS and 3DS regions did not decrease leaf rust severity in any of the tests compared to the susceptible parent, yet strongly interacted when present in the same genotype to decrease leaf rust severity equal to the resistant parent. Genotypes with only the 6DS region had significantly lower leaf rust severity compared to the susceptible parent in three of the field plot tests. The 6DS QTL also interacted with the 3DS QTL to decrease leaf rust severity. Major QTLs on chromosome 3AS and 3DS are both located in the distal region and may be encoded by homoeoalleles.
KeywordsAdult plant resistance Brown rust Wheat landrace Puccinia triticina Quantitative trait loci (QTL)
We thank K. Xiao and M. Hughes for excellent technical assistance. The authors would like to thank the IWGSC (www.wheatgenome.org) as well as project leaders Nils Stein, Curtis Pozniak, and Jesse Poland, for providing pre-publication access to IWGSC WGA v1.0. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture.
- Maccaferri M, Mantovani P, Tubersoa R, Deambrogia E, Giuliani S, Demontis A, Massi A, Sanguineti MC (2008) A major QTL for durable leaf rust resistance widely exploited in durum wheat breeding programs maps on the distal region of chromosome arm 7BL. Theor Appl Genet 117:1225–1240CrossRefPubMedGoogle Scholar
- McIntosh RA, Dubcovsky J, Rogers WJ, Morris, C 2017. Catalogue of gene symbols for wheat: 2017 supplement. Komugi Wheat Genetic Resources Database http://shigen.nig.ac.jp/wheat/komugi/genes/symbolClassList.jsp. Accessed Apr 4 2017
- Roelfs AP (1988) Resistance to leaf and stem rusts in wheat. In: Simmonds NW, Rajaram S (eds) Breeding strategies for resistance to the rusts of wheat. CIMMYT, Mexico, pp 10–22Google Scholar
- Roelfs AP, Singh RP, Saari EE (1992) Rust diseases of wheat: concepts and methods of disease management. CIMMYT, MexicoGoogle Scholar
- Singh A, Knox RE, DePauw RM, Singh AK, Cuthbert RD, Campbell HL, Singh D, Bhavani S, Fetch T, Clarke F (2013) Identification and mapping in spring wheat of genetic factors controlling stem rust resistance and the study of their interactions across multiple environmnents. Theor Appl Genet 126:1951–1964CrossRefPubMedPubMedCentralGoogle Scholar
- Wang S, Wong D, Forrest K, Allen A, Chao S, Huang BE, Maccaferri M, Salvi S, Milner SG, Cattivelli L, Mastrangelo AM, Whan A, Stephen S, Barker G, Wieseke R, Plieske J, International Wheat Genome Sequencing Consortium, Lillemo M, Mather D, Appels R, Dolferus R, Brown-Guedira G, Korol A, Akhunova AR, Feuillet C, Salse J, Morgante M, Pozniak C, Luo M-C, Dvorak J, Morell M, Dubcovsky J, Ganal M, Tuberosa R, Lawley C, Mikoulitch I, Cavanagh C, Edwards KJ, Hayden MJ, Akhunov E (2014) Characterization of polyploid wheat genomic diversity using a high-density 90 000 single nucleotide polymorphism array. Plant Biotechnol 12:787–796CrossRefGoogle Scholar