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Euphytica

, 214:55 | Cite as

Differential expression of hormone related genes between extreme segregants of a Saccharum interspecific F2 population

  • Fan Zhu
  • Ching Man Wai
  • Jisen Zhang
  • Tyler C. Jones
  • Chifumi Nagai
  • Ray Ming
Article
  • 122 Downloads

Abstract

Sugarcane is a highly productive, first generation biofuel feedstock, known for its remarkable efficiency in accumulating biomass. Hormones are important regulators for many biological processes in plants, especially in plant development and plant growth, which are crucial for plant biomass traits. To understand how hormones regulatory mechanisms contribute to sugarcane lignocellulose yield, we studied the transgressive segregation on biomass yield in the F2 population derived from a cross between Saccharum officinarum ‘LA Purple’ and Saccharum robustum ‘MOL5829’. Gene expression profiling was used to detect genes involved in three important hormone-related pathways, auxin, ethylene and gibberellin, to find out how they are differently regulated between the extreme segregants of high and low biomass yield groups. We identified seventeen differentially expressed genes in auxin, one in ethylene and one in gibberellin related signaling and biosynthesis pathways, which could potentially regulate biomass yield. Differentially expressed genes, PIF3 and EIL5, involved in gibberellin and ethylene pathway could play an important role in biomass accumulation. These plant hormone-related genes could serve as candidate genes in genetic modification and breeding programs to develop high yielding energy cane.

Keywords

Auxin Biomass Hormone regulation Transcriptome Gibberellin Ethylene 

Notes

Acknowledgments

This project was supported by grants from Energy Biosciences Institute, US DOE DE-SC0010686, the International Consortium for Sugarcane Biotechnology, and startup fund from Fujian Agriculture and Forestry University to RM.

Author contributions

RM and FZ conceived the study, RM coordinated all research activities, TJ and CN conducted field trials, FZ, CMW, and JZ carried out RNAseq experiment and bioinformatics analysis. FZ and RM wrote the manuscript; all authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Supplementary material

10681_2018_2137_MOESM1_ESM.docx (297 kb)
Supplementary material 1 (DOCX 297 kb)

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Authors and Affiliations

  1. 1.FAFU and UIUC-SIB Joint Center for Genomics and Biotechnology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Key Laboratory of Genetics, Breeding and Multiple Utilization of Corps, Ministry of EducationFujian Agriculture and Forestry UniversityFuzhouChina
  2. 2.Department of Plant BiologyUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  3. 3.Hawaii Agriculture Research CenterKuniaUSA

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