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Euphytica

, 214:57 | Cite as

Genotypic variability among cotton cultivars for heat and drought tolerance using reproductive and physiological traits

  • Kulvir Singh
  • Chathurika Wijewardana
  • Bandara Gajanayake
  • Suresh Lokhande
  • Ted Wallace
  • Don Jones
  • Kambham Raja Reddy
Article

Abstract

Development of rapid and inexpensive screening tools for heat and drought stress tolerance is needed and will be helpful in cotton breeding programs and selecting cultivars for a niche environment. In this study, several pollen-based traits at optimum and high temperatures and physiological parameters measured during the boll-filling period were used to evaluate variability among the cultivars for heat and drought stresses. Principal component analysis and drought stress response index methods were used to categorize cotton cultivars into three heat and drought tolerant clusters. Based on the combined analysis, PX532211WRF has been identified as heat- and drought-tolerant, and would be expected to perform better under both heat- and drought-stressed environments. A poor correlation between reproductive and physiological indices indicates that screening breeders have to use different traits to screen cultivars for reproductive and vegetative tolerance. Identified traits could serve as valuable screening tools in cotton breeding programs aimed at developing genotypes to a changing climate. Moreover, cultivar-dependent relative scores will aid in the identification of cultivars best suited to niche environments to alleviate the influences of abiotic stresses at both vegetative and reproductive stages.

Keywords

Heat stress Drought Screening tool Cumulative stress response index Pollen viability Pollen tube length 

Abbreviations

Pn

Leaf net photosynthesis

Fv′/Fm′

Chlorophyll fluorescence

gs

Stomatal conductance

ETR

Electron transport rate

WUE

Water use efficiency

Trans

Transpiration

CSI

Chlorophyll stability index

Chl.

Total chlorophyll

Car

Carotenoids

CMT

Cell membrane thermostability

SLA

Specific leaf area

CTD

Canopy temperature depression

PV

Pollen viability

PG

Pollen germination

PGTRI

Pollen germination temperature response index

IRI

Individual response index

CRI

Cumulative response index

HSRI

Heat stress response index

DSRI

Drought stress response index

HSRI-R

Heat stress response index-reproductive

HSRI-P

Heat stress response index-physiological

CHDSRI

Cumulative heat and drought response index

Notes

Acknowledgements

This work was partially supported by the USDA NIFA projects (2015-34263-24070, G-14901-1), Mississippi Agriculture, Forestry and Veterinary Medicine as part of undergraduate Research program, and Cotton Incorporated, Inc., Cary, NC. We also thank David Brand for technical help during the experiment. This study was a contribution of the Department of Plant and Soil Sciences, Mississippi State University, and the Mississippi Agricultural and Forestry Experiment Station.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

References

  1. Abro S, Rajput MT, Khan MA, Sial MA, Tahir SS (2015) Screening of cotton (Gossypium hirsutum L.) genotypes for heat tolerance. Pak J Bot 47:2085–2091Google Scholar
  2. Ashraf M, Saeed MM, Qureshi MJ (1994) Tolerance to high temperature in cotton (Gossypium hirsutum L.) at initial growth stages. Environ Exp Bot 34:275–283CrossRefGoogle Scholar
  3. Aslam M, Brown MS, Kohel RJ (1964) Evaluation of seven tetrazolium salts as vital pollen stains in cotton, Gossypium hirsutum L. Crop Sci 4:508–510CrossRefGoogle Scholar
  4. Azhar FM, Ali Z, Akhtar MM, Khan AA, Trethowan R (2009) Genetic variability of heat tolerance, and its effect on yield and fiber quality traits in upland cotton (Gossypium hirsutum L.). Plant Breed 128:356–362CrossRefGoogle Scholar
  5. Bibi AC, Oosterhuis DM, Brown RS, Gonias ED, Bourland FM (2003) The physiological response of cotton to high temperatures for germplasm screening. Summaries of arkansas cotton research. AAES Res Ser 521:87–93Google Scholar
  6. Brand D, Wijewardana C, Gao W, Reddy KR (2016) Interactive effects of carbon dioxide, low temperature, and ultraviolet-B radiation on cotton seedling root and shoot morphology and growth. Front Earth Sci 10:607–620CrossRefGoogle Scholar
  7. Burke JJ, Velten J, Oliver MJ (2004) In vitro analysis of cotton pollen germination. Agron J 96:359–368CrossRefGoogle Scholar
  8. Chapple CCS, Vogt T, Ellis BE, Somerville CR (1992) An Arabidopsis mutant defective in the general phenylpropanoid pathway. Plant Cell 4:1413–1424CrossRefPubMedPubMedCentralGoogle Scholar
  9. Christ EH, Webster PJ, Snider JL, Toma VE, Oosterhuis DM, Chastain DR (2016) Predicting heat stress in cotton using probabilistic canopy temperature forecasts. Agron J 108:1–11CrossRefGoogle Scholar
  10. Cornish K, Radin JW, Turcotte EL, Lu ZM, Zeiger E (1991) Enhanced photosynthesis and stomatal conductance of Pima cotton bred for increased yield. Plant Physiol 97:484–489CrossRefPubMedPubMedCentralGoogle Scholar
  11. Cross RH, McKay SAB, McHughen AG, Bonham-Smith PC (2003) Heat stress effects on reproduction and seed set in Linum usitatissimum L. (flax). Plant, Cell Environ 26:1013–1020CrossRefGoogle Scholar
  12. Dai A, Wigley TML, Boville BA, Kiehl JT, Buja LE (2001) Climates of the 20th and 21st centuries simulated by the NCAR climate system model. J Clim 14:485–519CrossRefGoogle Scholar
  13. Dodds DM, Buehring NW, Falconer LL, Golden BR, Shankle MW, Wallace TP (2016) Mississippi official cotton variety trials. http://www.mississippi-crops.com/wp-content/uploads/2016/12/2016-COTTON-OVT-BOOK.pdf
  14. Gajanayake B, Brian WT, Harkess RL, Reddy KR (2011) Screening ornamental pepper cultivars for temperature tolerance using pollen and physiological parameters. Hort Sci 46:878–884Google Scholar
  15. Golden BR, Clark WE, Dodds DM, Buehring NW, Martin SW, Shankle MW, Wallace TP (2012) Mississippi official cotton variety trials. http://www.mississippi-crops.com/wp-content/uploads/2013/02/Cotton-OVT-MS-2012-Final-Pub-for-Blog.pdf
  16. Golden BR, Clark WE, Dodds DM, Buehring NW, Falconer LL, Shankle MW, Wallace TP (2013) Mississippi official cotton variety trials. http://extension.msstate.edu/sites/default/files/publications/variety-trials/ib0485.pdf
  17. Gwata D, Wofford P, Pfahler P, Boote K (2003) Pollen morphology and in vitro germination characteristics of nodulating and non-nodulating soybean (Glycine max L.) genotypes. Theor Appl Genet 106:837–839CrossRefPubMedGoogle Scholar
  18. Hedhly A, Hormaza JI, Herrer M (2004) Effect of temperature on pollen tube kinetics and dynamics in sweet cherry, Prunus avium (Rosaceae). Am J Bot 91:558–564CrossRefPubMedGoogle Scholar
  19. Houghton JT, Ding Y, Griggs DJ (2001) Climate change 2001: the scientific basis. Contribution of working group to third assessment report to the Intergovernmental Panel on Climate Change (IPCC). Cambridge University Press, CambridgeGoogle Scholar
  20. Intergovernmental Panel on Climate Change (IPCC) (2007) Climate change 2007: impacts, adaptation, and vulnerability. In: Parry ML, Canziani OF, Palutikof JP, van der Linden PJ, Hanson CE (eds) Contribution of working group II to the fourth assessment report of the Intergovernmental Panel on Climate Change. Cambridge University Press, CambridgeGoogle Scholar
  21. Ismail MA, Hall AE (1999) Reproductive stage heat tolerance, leaf membrane thermostability and plant morphology in cowpea. Crop Sci 39:1762–1768CrossRefGoogle Scholar
  22. Jain M, Prasad PVV, Boote KJ, Hartwell AL, Chourey PS (2007) Effects of season-long high temperature growth conditions on sugar-to-starch metabolism in developing microspores of grain sorghum (Sorghum bicolor L. Moench). Planta 227:67–69CrossRefPubMedGoogle Scholar
  23. Jayaprakash P, Sarla N (2001) Development of an improved medium for germination of Cajanus cajan (L.) Millsp. pollen in vitro. J Exp Bot 52:851–855CrossRefPubMedGoogle Scholar
  24. Kakani VG, Prasad PVV, Craufurd PQ, Wheeler TR (2002) Response of in vitro pollen germination and pollen tube growth of groundnut (Arachis hypogaea L.) genotypes to temperature. Plant, Cell Environ 25:1651–1661CrossRefGoogle Scholar
  25. Kakani VG, Reddy KR, Koti S, Wallace TP, Prasad PVV, Reddy VR, Zhao D (2005) Differences in in vitro pollen germination and pollen tube growth of cotton cultivars in response to high temperature. Ann Bot 96:59–67CrossRefPubMedPubMedCentralGoogle Scholar
  26. Khan AI, Iftikhar AK, Sadaqat HA (2008) Heat tolerance is variable in cotton (Gossypium hirsutum L.) and can be exploited for breeding of better yielding cultivars under high temperature regimes. Pak J Bot 40:2053–2058Google Scholar
  27. Khan N, Azhar FM, Khan AA, Ahmad R (2014) Mea-surement of canopy temperature for heat toler-ance in upland cotton: variability and its genetic basis. Pak J Agri Sci 51:359–365Google Scholar
  28. Kolb PF, Robberecht R (1996) High temperature and drought stress effects on survival of Pinus ponderosa seedlings. Tree Physiol 16:665–672CrossRefPubMedGoogle Scholar
  29. Koti S, Reddy KR, Reddy VR, Kakani VG, Zhao D (2005) Interactive effects of carbon dioxide, temperature, and ultraviolet-B radiation on soybean (Glycine max L.) flower and pollen morphology, pollen production, germination, and tube lengths. J Exp Bot 56:725–736CrossRefPubMedGoogle Scholar
  30. Kuo CG, Chen HM, Sunday HC (1993) Membrane thermostability and heat tolerance of vegetable leaves. In: Kuo CG (ed) Adaptation of food crops to temperature and water stress. Asian Veg Res Dev Center, Shanhua, pp 160–168Google Scholar
  31. Liu Z, Yuan YL, Liu SQ, Yu XN, Rao LQ (2006) Screening for high-temperature tolerant cotton cultivars by testing in vitro pollen germination, pollen tube growth and boll retention. J Integr Plant Biol 48:706–714CrossRefGoogle Scholar
  32. Lokhande S, Reddy KR (2014) Quantifying temperature effects on cotton reproductive efficiency and fiber quality. Agron J 106:1275–1282CrossRefGoogle Scholar
  33. Lu Z, Zeiger E (1994) Selection of higher yield and heat resistance in pima cotton has caused genetically determined changes in stomatal conductance. Physiol Plant 92:273–278CrossRefGoogle Scholar
  34. Lu Z, Radin JW, Turcotte EL, Percy R, Zeiger E (1994) High yields in advanced lines of pima cotton are associated with higher stomatal conductance, reduced leaf area and lower leaf temperature. Physiol Plant 92:266–272CrossRefGoogle Scholar
  35. Lu Z, Percy RG, Qualset CO, Zeiger E (1998) Stomatal conductance predicts yields in irrigated Pima cotton and bread wheat grown at high temperatures. J Exp Bot 49:453–460CrossRefGoogle Scholar
  36. Malik MN, Chaudhry FI, Makhdum MI (1999) Cell membrane thermostability as a measure of heat-tolerance in cotton. Pak J Sci Ind Res 42:44–46Google Scholar
  37. Martineau JR, Williams JH, Specht JE (1979) Temperature tolerance in soybeans. II. Evaluation of segregating populations for membrane thermostability. Crop Sci 19:79–81CrossRefGoogle Scholar
  38. Meyer VG (1969) Some effects of genes, cytoplasm and environment on male sterility of cotton (Gossypium). Crop Sci 9:237–242CrossRefGoogle Scholar
  39. Mohan MM, Narayanan SL, Ibrahim SM (2000) Chlorophyll stability index (CSI): its impact on salt tolerance in rice. Intl Rice Res 25:38–39Google Scholar
  40. Noshair K, Azhar FM, Khan AA, Ahmad R (2014) Measurement of canopy temperature for heat tolerance in upland cotton: variability and its genetic basis. Pak J Agric Sci 51:359–365Google Scholar
  41. Oosterhuis DM (1997) Effects of temperature extremes on cotton yields in Arkansas. In: Oosterhuis DM (ed) Proceedings of Cotton Research Meeting and Research Summaries, University of Arkansas Agricultural experiment Station Special Report, vol 183, pp 94–98Google Scholar
  42. Paulsen GM (1994) High temperature responses of crop plants. In: Boote KJ, Bennett JM, Sinclair TR, Paulsen GM (eds) Physiology and determination of crop yield. American Society of Agronomy, Madison, pp 365–389Google Scholar
  43. Peet MM, Sato S, Gardner RG (1998) Comparing heat-stress effects on male-fertile and male-sterile tomatoes. Plant, Cell Environ 21:225–231CrossRefGoogle Scholar
  44. Percy RG, Lu ZM, Radin JW, Turcotte EL, Zeiger E (1996) Inheritance of performance of near isogenic lines from bulk populations. Crop Sci 26:219–222Google Scholar
  45. Pettigrew WT, Meredith WR Jr (1994) Leaf gas exchange parameters vary among cotton genotypes. Crop Sci 34:700–705CrossRefGoogle Scholar
  46. Porch TG, Jahn M (2001) Effects of high-temperature stress on microsporogenesis in heat-sensitive and heat-tolerant genotypes of Phaseolus vulgaris. Plant, Cell Environ 24:723–731CrossRefGoogle Scholar
  47. Prasad PVV, Craufurd PQ, Summerfield RJ (1999) Fruit number in relation to pollen production and viability in groundnut exposed to short episodes of heat stress. Ann Bot 84:381–386CrossRefGoogle Scholar
  48. Prasad PVV, Boote KJ, Allen H, Thomas JMG (2002) Effect of elevated temperature and carbon dioxide on seed set and yield of kidney bean (Phaseolus vulgaris L.). Glob Change Biol 8:710–721CrossRefGoogle Scholar
  49. Prasad PVV, Boote KJ, Allen H, Thomas JMG (2003) Super-optimal temperatures are detrimental to peanut (Arachis hypogaea L.) reproductive processes and yield at both ambient and elevated carbon dioxide. Glob Change Biol 9:1775–1787CrossRefGoogle Scholar
  50. Radin JW, Lu ZM, Percy RG, Zeiger E (1994) Genetic variation for stomatal conductance in Pima cotton and its relation to improvements of heat adaptation. Proc Natl Acad Sci USA 91:7217–7221CrossRefPubMedPubMedCentralGoogle Scholar
  51. Reddy KR, Hodges HF (2000) Climate change and global crop productivity. CAB International, OxonCrossRefGoogle Scholar
  52. Reddy KR, Kakani VG (2007) Screening Capsicum species of different origins for high temperature tolerance by in vitro pollen germination and pollen tube length. Hort Sci 112:130–135CrossRefGoogle Scholar
  53. Reddy VR, Reddy KR, Hodges HF (1991) Temperature effects on growth and development of cotton during the fruiting period. Agron J 83:211–217CrossRefGoogle Scholar
  54. Reddy KR, Hodges HF, Reddy VR (1992a) Temperature effects on cotton fruit retention. Agron J 84:26–30CrossRefGoogle Scholar
  55. Reddy KR, Reddy VR, Hodges HF (1992b) Temperature effects on early season cotton growth and development. Agron J 84:229–237CrossRefGoogle Scholar
  56. Reddy KR, Hodges HF, McKinion JM (1993) A temperature model for cotton phenology. Biotronics 22:47–59Google Scholar
  57. Reddy KR, Hodges HF, McKinion JM (1995) Carbon dioxide and temperature effects on pima cotton growth. Agric Ecosyst Environ 54:17–29CrossRefGoogle Scholar
  58. Reddy KR, Hodges HF, McKinion JM (1997) A comparison of scenarios for the effect of global climate change on cotton growth and yield. Aust J Plant Phys 24:707–713CrossRefGoogle Scholar
  59. Reddy KR, Davidonis GH, Johnson AS, Vinyard BT (1999) Temperature regime and carbon dioxide enrichment alter cotton boll development and fiber properties. Agron J 91:851–858CrossRefGoogle Scholar
  60. Reddy KR, Brand D, Wijewardana C, Gao W (2017) Temperature effects on cotton seedling emergence, growth, and development. Agron J 109:1379–1387CrossRefGoogle Scholar
  61. Rodriguez-Garay B, Barrow JR (1988) Pollen selection for heat tolerance in cotton. Crop Sci 28:857–859CrossRefGoogle Scholar
  62. Saini HS, Sedgley M, Aspinall D (1983) Effect of heat stress during floral development on pollen tube growth and ovary anatomy in wheat (Triticum aestivum L.). Aust J Plant Physiol 10:137–144CrossRefGoogle Scholar
  63. Sairam RK, Deshmukh PS, Shukla DS (1997) Increased antioxidant enzyme activity in response to drought and temperature stress related with stress tolerance in wheat genotypes, Abstract: National Seminar (ISSP), IARI, New Delhi p 69Google Scholar
  64. Salem MA, Kakani VG, Koti S, Reddy KR (2007) Pollen-based screening of soybean genotypes for high temperatures. Crop Sci 47:219–231CrossRefGoogle Scholar
  65. Sari-Gorla M, Pe ME, Rossini L (1994) Detection of QTLs controlling pollen germination and growth in maize. Heredity 72:332–335CrossRefGoogle Scholar
  66. Singh RP, Prasad PVV, Sunita K, Giri SN, Reddy KR (2007) Influence of high temperature and breeding for heat tolerance in cotton: a review. Adv Agron 93:313–385CrossRefGoogle Scholar
  67. Singh SK, Kakani VG, Brand D, Baldwin B, Reddy KR (2008) Assessment of cold and heat tolerance of winter-grown canola (Brassica napus L.) cultivars by pollen-based parameters. J Agron Crop Sci 194:225–236CrossRefGoogle Scholar
  68. Snider JL, Oosterhuis DM (2012) Heat stress and pollen–pistil interactions. In: Oosterhuis DM, Cothren JT (eds) Flowering and fruiting in cotton. The Cotton Foundation, Cordova, pp 59–78Google Scholar
  69. Snider JL, Oosterhuis DM (2015) Physiology. In: Fang D, Percy R (eds) Agronomy, monograph 57, cotton, 2nd edn. ASA-CSSA-SSSA, Madison, pp 339–400Google Scholar
  70. Snider JL, Oosterhuis DM, Skulman BW, Kawakami EM (2009) Heat stress-induced limitations to reproductive success in Gossypium hirsutum. Physiol Plant 137:125–138CrossRefPubMedGoogle Scholar
  71. Sullivan CY (1972) Mechanism of heat and drought resistance in grain sorghum and methods of measurement. In: Rao NGP, House LR (eds) Sorghum in the Seventies. Oxford and IBH Publishing Co., New DelhiGoogle Scholar
  72. Talwar HS, Yanagihara S (1999) Physiological basis for heat tolerance during flowering and pod setting stages in groundnut (Arachis hypogaea L.). JIRCAS Work Rep 14:47–65Google Scholar
  73. ur Rahman H, Malik SA, Saleem M (2004) Heat tolerance of upland cotton during the fruiting stage evaluated using cellular membrane thermostability. Field Crops Res 85:149–158CrossRefGoogle Scholar
  74. Wijewardana C, Hock M, Henry WB, Reddy KR (2015) Screening corn hybrids for cold tolerance using morphological traits for early season seeding. Crop Sci 19:75–78Google Scholar
  75. Wijewardana C, Henry WB, Gao W, Reddy KR (2016a) Interactive effects on CO2, drought, and ultraviolet-B radiation on maize growth and development. Photochem Photo Biol 160:198–209CrossRefGoogle Scholar
  76. Wijewardana C, Henry WB, Hock M, Reddy KR (2016b) Growth and physiological trait variation among corn (Zea mays L.) hybrids for cold tolerance. Can J Plant Sci 96:639–656CrossRefGoogle Scholar
  77. Wijewardana C, Henry WB, Reddy KR (2017) Evaluation of drought tolerant maize germplasm in response to induced drought stress. Missi Aca Sci 62:316–329Google Scholar
  78. Young L, Wilen R, Bonham-Smith P (2004) High temperature stress of Brassica napus during flowering reduces micro- and megagametophyte fertility, induces fruit abortion, and disrupts seed production. J Expt Bot 55:485–495CrossRefGoogle Scholar
  79. Zahid KR, Ali F, Shah F, Younas M, Shah T, Shahwar D, Hassan W, Ahmad Z, Qi C, Iqbal A, Wu W (2016) Response and tolerance mechanism of cotton Gossypium hirsutum L. to elevated temperature stress: a review. Front Plant Sci 7:937CrossRefPubMedPubMedCentralGoogle Scholar

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Authors and Affiliations

  1. 1.Department of Plant and Soil SciencesMississippi State UniversityMississippi StateUSA
  2. 2.Agricultural and Environmental ResearchCotton IncorporatedCaryUSA

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