Production and genetic analyses of novel Brassica rapa L. introgressions from interspecific crosses with Brassica juncea L. landraces native to the Qinghai-Tibet Plateau Article First Online: 10 January 2018 Received: 20 July 2017 Accepted: 03 January 2018 Abstract
Interspecific hybrids between related species have long been used for transferring desirable genes, broadening genetic diversity and utilizing intersubgenomic heterosis. In this study, we developed a novel
Brassica rapa type (AA, 2n = 20) exhibiting certain features derived from interspecific hybridization between natural B. rapa and Brassica juncea (AABB, 2n = 36). In pollen mother cells (PMCs) of the novel B. rapa type, normal chromosome pairing with 10 bivalents and 10:10 segregation was observed, and the novel B. rapa lines were completely fertile. However, GISH showed that certain B chromosomes or fragments were introgressed into B. rapa. Genetic components of the novel B. rapa lines were investigated by GISH, AFLP and SSR analyses. GISH analysis of F 1, BC 1F 1, and BC 1F 2 plants confirmed the identities of three addition lines and seven translocation lines. AFLP and SSR analyses of 60 hybrid progenies from BC 1F 4 plants, their parents, and some B. juncea and B. rapa resources indicated that the A J and B chromosome(s) or fragment(s) introgressed to the novel B. rapa. AFLP revealed that 60 BC 1F 4 plants contained B chromosomes or fragments, which evidenced introgression into the hybrid progeny. SSR analysis indicated that the A-genome (A1–A10) of B. juncea was introgressed into the hybrid progeny at 1.0 to 42.7%. Lastly, we obtained some yellow-seed and early-flowering B. rapa resources. The novel B. rapa lines can be used to genetically improve B. rapa in the Qinghai-Tibet Plateau and to study the origin and evolution of the A- and B-genomes. Keywords Amplified fragment length polymorphism (AFLP) B. juncea B. rapa Genetic introgression Genomic in situ hybridization (GISH) Simple sequence repeat (SSR)
Changcai Teng and Yan Niu are equally contributed to the work and should be regarded as co-first authors.
Electronic supplementary material
The online version of this article (
) contains supplementary material, which is available to authorized users. https://doi.org/10.1007/s10681-018-2108-4 Notes Acknowledgements
The authors are grateful to Dr. Bin Zhu for his critical reading of the manuscript.
ZZ and DD designed and managed this study. CT and YN performed the experiments and analyzed the data. CT wrote the manuscript. QY designed and executed the artificial synthesis of
B. rapa. Compliance with ethical standards Conflict of interest
We declare that we have no financial or personal relationships with other people or organizations that can inappropriately influence our work. There are no professional or other personal interests of any nature in any product, service and/or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled, “Production and genetic analyses of novel
Brassica rapa L. introgressions from interspecific crosses with Brassica juncea L. landraces native to the Qinghai-Tibet Plateau”. Ethical approval
This article does not describe any studies involving human participants or animals.
Anamthawat-Jonsson K (2001) Molecular cytogenetics of introgression hybridization in plants. Methods Cell Sci 23:139–148.
https://doi.org/10.1023/A:1013182724179 CrossRef PubMed Google Scholar
Attia T, Robbelen G (1986) Cytogenetic relationship within cultivated
analyzed in amphihaploids from the three diploid ancestor. Can J Genet Cytol 28:323–329.
https://doi.org/10.1139/g86-048 CrossRef Google Scholar
Attia T, Busso C, Robbelen G (1987) Digenomic triploids for an assessment of chromosome relationships in the cultivated diploid
species. Genome 29:326–330.
https://doi.org/10.1139/g87-053 CrossRef Google Scholar
Barba-Gonzalez R, Van Silfhout AA, Visser RGF, Ramanna MS, Van Tuyl JM (2006) Progenies of allotriploids of Oriental × Asiatic lilies (
) examined by GISH analysis. Euphytica 151:243–250.
https://doi.org/10.1007/s10681-006-9148-x CrossRef Google Scholar
Bennett RA, Séguin-Swartz G, Rahman H (2012) Broadening genetic diversity in Canola using the C-genome species
L. Crop Sci 52:2030–2039.
https://doi.org/10.2135/cropsci2011.11.0580 CrossRef Google Scholar
Chen HF, Wang H, Li ZY (2007) Production and genetic analysis of partial hybrids in intertribal crosses between
B. rapa, B. napus
. Plant Cell Rep 26:1791–1800.
https://doi.org/10.1007/s00299-007-0392-x CrossRef PubMed Google Scholar
Choudhary BR, Joshi P, Rao SR (2002) Cytogenetics of
hybrids and patterns of variation in the hybrid derivatives. Plant Breed 121:292–296.
https://doi.org/10.1046/j.1439-0523.2002.00715.x CrossRef Google Scholar
Cui C, Ge X, Gautam M, Kang L, Li Z (2012) Cytoplasmic and genomic effects on meiotic pairing in
hybrids and allotetraploids from pair crosses of three cultivated diploids. Genetics 191:725–738.
https://doi.org/10.1534/genetics.112.140780 CrossRef PubMed PubMedCentral Google Scholar
Dhaka N, Rout K, Yadava SK, Sodhi YS, Gupta V, Pental D, Pradhan AK (2017) Genetic dissection of seed weight by QTL analysis and detection of allelic variation in Indian and East European gene pool lines of
. Theor Appl Genet 130:293–307.
https://doi.org/10.1007/s00122-016-2811-2 CrossRef PubMed Google Scholar
Du XZ, Ge XH, Yao XC, Zhao ZG, Li ZY (2009) Production and cytogenetic characterization of intertribal somatic hybrids between
and backcross progenies. Plant Cell Rep 28:1105–1113.
https://doi.org/10.1007/s00299-009-0712-4 CrossRef PubMed Google Scholar
Fredua-Agyeman R, Coriton O, Huteau V, Parkin IA, Chèvre AM, Rahman H (2014) Molecular cytogenetic identification of B genome chromosomes linked to blackleg disease resistance in
interspecific hybrids. Theor Appl Genet 127:1305–1318.
https://doi.org/10.1007/s00122-014-2298-7 CrossRef PubMed Google Scholar
Fu CH, Chen CL, Guo WW, Deng XX (2004) GISH, AFLP and PCR-RFLP analysis of an intergeneric somatic hybrid combining Goutou sour orange and
. Plant Cell Rep 23:391–396.
https://doi.org/10.1007/s00299-004-0828-5 CrossRef PubMed Google Scholar
González G, Comas C, Confalonieri V, Naranjo CA, Poggio L (2006) Genomic affinities between maize and
using classical and molecular cytogenetic methods (GISH–FISH). Chromosome Res 14:629–635.
https://doi.org/10.1007/s10577-006-1072-3 CrossRef PubMed Google Scholar
Guttman B (2001) Evolution. In: Brenner S, Miller JH (eds) Encyclopedia of genetics, vol 2. Academic, San Diego, pp 663–666
CrossRef Google Scholar
Hua YW, Li ZY (2006) Genomic in situ hybridization analysis of
hybrids and production of
aneuploids. Plant Breed 125:144–149.
https://doi.org/10.1111/j.1439-0523.2006.01200.x CrossRef Google Scholar
Hua YW, Liu M, Li ZY (2006) Parental genome separation and elimination of cells and chromosomes revealed by AFLP and GISH analyses in a
cross. Ann Bot 97:993–998.
https://doi.org/10.1093/aob/mcl073 CrossRef PubMed PubMedCentral Google Scholar
Jiang J, Gill BS (1994) Nonisotopic in situ hybridization and plant genome mapping: the first 10 years. Genome 37:717–725.
https://doi.org/10.1139/g94-102 CrossRef PubMed Google Scholar
Kang L, Du X, Zhou Y, Zhu B, Ge X, Li Z (2014) Development of a complete set of monosomic alien addition lines between
(Chinese woad). Plant Cell Rep 33:1355–1364.
https://doi.org/10.1007/s00299-014-1621-8 CrossRef PubMed Google Scholar
Leflon M, Eber F, Letanneur JC, Chelysheva L, Coriton O, Huteau V, Ryder CD, Barker G, Jenczewski E, Chèvre AM (2006) Pairing and recombination at meiosis of
(AACC) hybrids. Theor Appl Genet 113:1467–1480.
https://doi.org/10.1007/s00122-006-0393-0 CrossRef PubMed Google Scholar
Li Z, Liu HL, Luo P (1995) Production and cytogenetics of intergeneric hybrids between
. Theor Appl Genet 91:131–136.
https://doi.org/10.1007/BF00220869 PubMed Google Scholar
Li MT, Li ZY, Zhang CY, Qian W, Meng JL (2005) Reproduction and cytogenetic characterization of interspecific hybrids derived from crosses between
. Theor Appl Genet 110:1284–1289.
https://doi.org/10.1007/s00122-005-1965-0 CrossRef PubMed Google Scholar
Li M, Chen X, Meng J (2006) Intersubgenomic heterosis in rapeseed production with a partial new-typed
containing subgenome Ar from
and Cc from
. Crop Sci 46:234–242.
https://doi.org/10.2135/cropsci2004.0759 CrossRef Google Scholar
Li Q, Mei J, Zhang Y, Li J, Ge X, Li Z, Qian W (2013) A large-scale introgression of genomic components of
by the bridge of hexaploid derived from hybridization between
. Theor Appl Genet 126:2073–2080.
https://doi.org/10.1007/s00122-013-2119-4 CrossRef PubMed Google Scholar
Li G, Lang T, Dai G, Li D, Li C, Song X, Yang Z (2015) Precise identification of two wheat–
substitutions reveals the compensation and rearrangement between wheat and
chromosomes. Mol Breed 35:1–10.
https://doi.org/10.1007/s11032-015-0202-z CrossRef Google Scholar
Ma N, Li ZY, Cartagena JA, Fukui K (2006) GISH and AFLP analyses of novel
lines derived from one hybrid between
. Plant Cell Rep 25:1089–1093.
https://doi.org/10.1007/s00299-006-0171-0 CrossRef PubMed Google Scholar
Mason AS, Huteau V, Eber F, Coriton O, Yan G, Nelson MN, Cowling W, Chèvre A (2010) Genome structure affects the rate of autosyndesis and allosyndesis in AABC, BBAC and CCAB
interspecific hybrids. Chromosome Res 18:655–666.
https://doi.org/10.1007/s10577-010-9140-0 CrossRef PubMed Google Scholar
Molnár I, Molnár-Láng M (2010) GISH reveals different levels of meiotic pairing with wheat for individual
chromosomes. Biol Plant 54:259–264.
https://doi.org/10.1007/s10535-010-0045-8 CrossRef Google Scholar
Nagaharu U (1935) Genome analysis in
with special reference to the experimental formation of
and peculiar mode of fertilization. Jpn J Bot 7:389–452
Navabi ZK, Parkin IA, Pires JC, Xiong Z, Thiagarajah MR, Good AG, Rahman MH (2010) Introgression of B-genome chromosomes in a doubled haploid population of
. Genome 53:619–629.
https://doi.org/10.1139/g10-039 CrossRef PubMed Google Scholar
Navabi ZK, Stead KE, Pires JC, Xiong Z, Sharpe AG, Parkin IAP, Rahman MH, Good AG (2011) Analysis of B-Genome chromosome introgression in interspecific hybrids of
. Genetics 187:659–673.
https://doi.org/10.1534/genetics.110.124925 CrossRef PubMed PubMedCentral Google Scholar
Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endouncleases. Proc Natl Acad Sci 76:5269–5273
CrossRef PubMed PubMedCentral Google Scholar
Rahman H, Bennett RA, Séguin-Swartz G (2015) Broadening genetic diversity in
canola: development of canola-quality spring
interspecific crosses. Can J Plant Sci 95:29–41.
https://doi.org/10.4141/CJPS-2014-017 CrossRef Google Scholar
Röbbelen G (1960) Beitrage zur analyse des
-genoms. Chromosoma 11:205–228.
https://doi.org/10.1007/BF00328652 CrossRef Google Scholar
Rohlf FJ (1997) NTSYS-pc 2.1. Numerical taxonomy and multivariate analysis system. Exeter Software, Setauket
Tan C, Cui C, Xiang Y, Ge X, Li Z (2017) Development of
monosomic alien addition lines: genotypic, cytological and morphological analyses. Theor Appl Genet.
https://doi.org/10.1007/s00122-017-2971-8 PubMedCentral Google Scholar
Tang X, Shi D, Xu J, Li Y, Li W, Ren Z, Fu T (2014) Molecular cytogenetic characteristics of a translocation line between common wheat and
with resistance to powdery mildew. Euphytica 197:201–210.
https://doi.org/10.1007/s10681-013-1059-z CrossRef Google Scholar
Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414.
https://doi.org/10.1093/nar/23.21.4407 CrossRef PubMed PubMedCentral Google Scholar
Waara S, Glimelius K (1995) The potential of somatic hybridization in crop breeding. Euphytica 85:217–233.
https://doi.org/10.1007/BF00023951 CrossRef Google Scholar
Wang YP, Snowdon RJ, Rudloff E, Wehling P, Friedt W, Sonntag K (2004) Cytogenetic characterization and
gene variation in progenies from asymmetric somatic hybrids between
. Genome 47:724–731.
https://doi.org/10.1139/g04-024 CrossRef PubMed Google Scholar
Warude D, Chavan P, Joshi K, Patwardhan B (2003) DNA isolation from fresh, dry plant samples with highly acidic tissue extracts. Plant Mol Biol Rep 21:467.
https://doi.org/10.1007/BF02772600 CrossRef Google Scholar
Wen J, Tu J, Li Z, Fu T, Ma C, Shen J (2008) Improving ovary and embryo culture techniques for efficient resynthesis of
from reciprocal crosses between yellow-seeded diploids
. Euphytica 162:81–89.
https://doi.org/10.1007/s10681-007-9566-4 CrossRef Google Scholar
Woods DL, Capcara JJ, Downey RK (1991) The potential of mustard (
(L.) Coss) as an edible oil crop on the Canadian Prairies. Can J Plant Sci 71:195–198.
https://doi.org/10.4141/cjps91-025 CrossRef Google Scholar
Xie S, Khan N, Ramanna MS, Niu L, Marasek-Ciolakowska A, Arens P, van Tuyl JM (2010) An assessment of chromosomal rearrangements in neopolyploids of
hybrids. Genome 53:439–446.
https://doi.org/10.1139/g10-018 CrossRef PubMed Google Scholar
Yamashita K, Takatori Y, Tashiro Y (2005) Chromosomal location of a pollen fertility-restoring gene,
, for CMS in Japanese bunching onion (
L.) possessing the cytoplasm of
Kar. et Kir. revealed by genomic in situ hybridization. Theor Appl Genet 111:15–22.
https://doi.org/10.1007/s00122-005-1941-8 CrossRef PubMed Google Scholar
Yao XC, Ge XH, Chen JP, Li ZY (2010) Intra- and intergenomic relationships in interspecific hybrids between
) and a wild species
as revealed by genomic in situ hybridization (GISH). Euphytica 173:113–120.
https://doi.org/10.1007/s10681-010-0131-1 CrossRef Google Scholar
Younis A, Ramzan F, Hwang YJ, Lim KB (2015) FISH and GISH: molecular cytogenetic tools and their applications in ornamental plants. Plant Cell Rep 34:1477–1488.
https://doi.org/10.1007/s00299-015-1828-3 CrossRef PubMed Google Scholar
Zhong XB, Hans de Jong JH, Zabel P (1996) Preparation of tomato meiotic pachytene and mitotic metaphase chromosomes suitable for fluorescence in situ hybridization (FISH). Chromosome Res 4:24–28.
https://doi.org/10.1007/BF02254940 CrossRef PubMed Google Scholar
Zhou J, Yang Z, Li G, Liu C, Tang Z, Zhang Y, Ren Z (2010) Diversified chromosomal distribution of tandemly repeated sequences revealed evolutionary trends in
). Plant Syst Evol 287:49–56.
https://doi.org/10.1007/s00606-010-0288-z CrossRef Google Scholar
Zhu JS, Struss D, Röbbelen G (1993) Studies on resistance to
addition lines. Plant Breed 111:192–197.
https://doi.org/10.1111/j.1439-0523.1993.tb00629.x CrossRef Google Scholar Copyright information
© Springer Science+Business Media B.V., part of Springer Nature 2018