Production and genetic analyses of novel Brassica rapa L. introgressions from interspecific crosses with Brassica juncea L. landraces native to the Qinghai-Tibet Plateau
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Interspecific hybrids between related species have long been used for transferring desirable genes, broadening genetic diversity and utilizing intersubgenomic heterosis. In this study, we developed a novel Brassica rapa type (AA, 2n = 20) exhibiting certain features derived from interspecific hybridization between natural B. rapa and Brassica juncea (AABB, 2n = 36). In pollen mother cells (PMCs) of the novel B. rapa type, normal chromosome pairing with 10 bivalents and 10:10 segregation was observed, and the novel B. rapa lines were completely fertile. However, GISH showed that certain B chromosomes or fragments were introgressed into B. rapa. Genetic components of the novel B. rapa lines were investigated by GISH, AFLP and SSR analyses. GISH analysis of F1, BC1F1, and BC1F2 plants confirmed the identities of three addition lines and seven translocation lines. AFLP and SSR analyses of 60 hybrid progenies from BC1F4 plants, their parents, and some B. juncea and B. rapa resources indicated that the AJ and B chromosome(s) or fragment(s) introgressed to the novel B. rapa. AFLP revealed that 60 BC1F4 plants contained B chromosomes or fragments, which evidenced introgression into the hybrid progeny. SSR analysis indicated that the A-genome (A1–A10) of B. juncea was introgressed into the hybrid progeny at 1.0 to 42.7%. Lastly, we obtained some yellow-seed and early-flowering B. rapa resources. The novel B. rapa lines can be used to genetically improve B. rapa in the Qinghai-Tibet Plateau and to study the origin and evolution of the A- and B-genomes.
KeywordsAmplified fragment length polymorphism (AFLP) B. juncea B. rapa Genetic introgression Genomic in situ hybridization (GISH) Simple sequence repeat (SSR)
The authors are grateful to Dr. Bin Zhu for his critical reading of the manuscript.
ZZ and DD designed and managed this study. CT and YN performed the experiments and analyzed the data. CT wrote the manuscript. QY designed and executed the artificial synthesis of B. rapa.
This study was financially supported by funds from the National Key Research and Development Plan of China (2016YFD0100202), the Key Laboratory of Spring Rape Genetic Improvement of Qinghai Province (2017-ZJ-Y09) and the Industry Technology Systems for Rapeseed in China (CARS-13).
Compliance with ethical standards
Conflict of interest
We declare that we have no financial or personal relationships with other people or organizations that can inappropriately influence our work. There are no professional or other personal interests of any nature in any product, service and/or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled, “Production and genetic analyses of novel Brassica rapa L. introgressions from interspecific crosses with Brassica juncea L. landraces native to the Qinghai-Tibet Plateau”.
This article does not describe any studies involving human participants or animals.
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