Euphytica

, 214:34 | Cite as

Screening A. ventricosa populations for 2n gametes

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Abstract

Sexual polyploidization via the formation of 2n gametes has been acknowledged as the most significant evolutionary mode of polyploidization among plant species. The present study was conducted in order to determine whether 2n gametes are present in the C-genome diploid Avena ventricosa Bal. ex Coss., a species that contributed to the evolution of the cultivated hexaploid species (Avena sativa L). Individual plants belonging to four different Cypriot populations, were screened for pollen grain size variation with the aim to distinguish 2n gametes. Avena ventricosa ARI00-845 was identified to produce large pollen grains at a low percentage (1.21%). Subsequent analysis using flow cytometry confirmed the presence of 2n gametes in the pollen. Cytogenetic analyses of pollen mother cells revealed cells with twice the typical chromosome number at metaphase I (i.e., 28 chromosomes). We postulate that irregularities in cell wall formation preceding meiosis could have contributed to the mode of chromosome doubling.

Keywords

Flow cytometry Meiosis Oats Pollen mother cells Unreduced gametes 

Notes

Author contributions

NN and AK designed the study and wrote the manuscript. NN and AA conducted experiments. NN and AA participated in data analysis. All authors read and approved the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Agricultural Science, Biotechnology and Food ScienceCyprus University of TechnologyLimassolCyprus
  2. 2.Laboratory of Biology and Physiology of Organisms, Biosystematics Genetics & EvolutionUniversity of Science and Technology Houari BoumedieneBab-EzzouarAlgeria

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