Euphytica

, 214:18 | Cite as

Mining the global diversity of barley for Fusarium resistance using leaf and spike inoculations

  • Ismail M. A. Bedawy
  • Heinz-Wilhelm Dehne
  • Jens Léon
  • Ali Ahmad Naz
Article
  • 140 Downloads

Abstract

Fusarium graminearum is a devastating fungal pathogen that causes significant yield and quality losses in cereals. We utilized a diversity set of barley (140 genotypes) to explore vital resistance alleles against this aggressive pathogen. The resistance assessment was carried out on spikes and leaves via artificial inoculations under control conditions. The phenotypic data was subjected to genome-wide association analysis using a genetic map based on DArT and SNP markers. This analysis revealed eleven and nine marker trait associations for leaf disease scoring (LDS) and spike disease scoring (SDS), respectively. The strongest QTL for LDS was found on chromosome 1H where a minor allele of wild origin decreased disease symptoms by 78%. The major QTL allele for SDS was linked with marker locus SCRI_RS174710 on chromosome 5H. In addition, four favorable epistatic interactions effects were found in decreasing disease symptoms. Overall, three QTL were common for LDS and SDS, which indicates a partial genetic relatedness of these resistances in barley. The QTL alleles for LDS and SDS will help to establish organ specific resistances in cultivated barley.

Keywords

Fusarium graminearum Triticum aestivum Resistance QTL GWAS Leaf and spike disease symptoms 

Supplementary material

10681_2017_2103_MOESM1_ESM.tif (313 kb)
Plot of linkage disequilibrium (LD) presented by r2 against genetic distance (in centiMorgan) Supplementary material 1 (TIFF 312 kb)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  • Ismail M. A. Bedawy
    • 1
    • 3
  • Heinz-Wilhelm Dehne
    • 2
  • Jens Léon
    • 1
  • Ali Ahmad Naz
    • 1
  1. 1.Institute of Crop Science and Resource Conservation, Chair of Plant BreedingUniversity of BonnBonnGermany
  2. 2.Institute of Crop Science and Resource Conservation, Chair of Plant PathologyUniversity of BonnBonnGermany
  3. 3.Department of Agronomy, Faculty of AgricultureSohag UniversitySohagEgypt

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