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Euphytica

, 214:15 | Cite as

Identification of quantitative trait loci for agronomic and physiological traits in maize (Zea mays L.) under high-nitrogen and low-nitrogen conditions

  • Kunhui He
  • Liguo Chang
  • Yuan Dong
  • Tingting Cui
  • Jianzhou Qu
  • Xueyan Liu
  • Shutu Xu
  • Jiquan Xue
  • Jianchao Liu
Article
  • 278 Downloads

Abstract

Low-nitrogen (LN) tolerance is a compound character with a complex genetic basis. Many agronomic traits have been shown to be closely related to LN tolerance in maize. In this study, 150 F7 recombinant inbred lines derived from a cross between inbreds 178 and K12 were evaluated for agronomical and physiological traits under high-nitrogen (HN) and LN conditions in 2 years. Inclusive composite interval mapping (ICIM) was used to identify the quantitative trait loci (QTLs) for traits recorded under different treatments (LN and HN) in 2 years. In total, 86 QTLs were detected: 38 for HN and 35 for LN, while 13 QTLs were detected under both nitrogen levels, suggesting that LN-specific QTLs may play a role in improving LN tolerance in maize. Overlapping QTLs for different traits were located on all chromosomes except chromosome 4 and chromosome 9. Many of these regions overlapped with previously reported QTLs. Several consensus major QTLs and LN-specific major QTLs found in the study can be used in marker-assisted selection breeding for genetic improvement and LN tolerance in maize in the future.

Keywords

Maize Agronomic traits Low-nitrogen tolerance LN-specific QTL 

Abbreviations

BLUP

Best linear unbiased prediction

EH

Ear height

GLN

Green leaf number

GNE

Grain number per ear

GYP

Grain yield per plant

HN

High-nitrogen

LA

Leaf area

LL

Leaf length

LN

Low-nitrogen

LW

Leaf width

PH

Plant height

QTL

Quantitative trait loci

RILs

Recombinant inbred lines

SPAD-BEL

Relative chlorophyll content of the lower ear leaf

SPAD-EL

Relative chlorophyll content of the ear leaf

SPAD-UEL

Relative chlorophyll content of the upper ear leaf

Notes

Acknowledgements

This study was supported financially by the National Science Foundation of China (No. 31301830), Natural Science Basic Research Plan in Shaanxi Province of China (No. 2014JQ3108), and Special Fund for Basic Research in Northwest A&F University (No. QN2012001).

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  • Kunhui He
    • 1
  • Liguo Chang
    • 1
  • Yuan Dong
    • 1
  • Tingting Cui
    • 1
  • Jianzhou Qu
    • 1
  • Xueyan Liu
    • 1
  • Shutu Xu
    • 1
  • Jiquan Xue
    • 1
  • Jianchao Liu
    • 1
  1. 1.Key Laboratory of Biology and Genetic Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture, Maize Engineering & Technology Research Centre of Shaanxi Province, College of AgronomyNorthwest A&F UniversityYanglingChina

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