Natural variation in photoperiodic flowering pathway and identification of photoperiod-insensitive accessions in wild wheat, Aegilops tauschii
- 264 Downloads
The D-genome progenitor of hexaploid wheat, Aegilops tauschii Coss., has a wide natural species range in central Eurasia and possesses wide natural variation in heading and flowering time. Here, we report identification of two Ae. tauschii accessions insensitive to short day length. Similarly to a loss or reduced degree of vernalization requirement, the photoperiod-insensitive mutations were found only in the early flowering sublineage (TauL1b) of Ae. tauschii. Quantitative trait locus (QTL) analyses using two F2 mapping populations showed that a QTL for heading time on the long arm of chromosome 5D was related to the early heading phenotype of the photoperiod-insensitive accessions under short-day conditions. In the photoperiod-insensitive accession, expression patterns of two flowering-related genes were altered under short-day conditions compared with the patterns in photoperiod-sensitive accessions. This study indicates that analysis of natural variations in the Ae. tauschii population is useful to find novel genetic loci controlling agronomically important traits.
KeywordsFlowering time Natural variation Photoperiod sensitivity Quantitative trait locus Wheat
This work was financially supported by Grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (Grants-in-Aid for Scientific Research (B) Nos. 21380005 and 16H04862).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Jones H, Gosman N, Horsnell R, Rose GA, Everst LA, Bentley AR, Tha S, Uauy C, Kowalski A, Novoselovic D, Simek R, Kobiljski B, Kondic-Spika A, Brbaklic L, Mitrofanova O, Chesnokov Y, Bonnett D, Greenland A (2013) Strategy for exploiting exotic germplasm using genetic, morphological, and environmental diversity: the Aegilops tauschii Coss. example. Theor Appl Genet 126:1793–1808CrossRefPubMedGoogle Scholar
- Matsuoka Y, Nasuda S, Ashida Y, Nitta M, Tsujimoto H, Takumi S, Kawahara T (2013) Genetic basis for spontaneous hybrid genome doubling during allopolyploid speciation of common wheat shown by natural variation analyses of the paternal species. PLoS ONE 8:e68310CrossRefPubMedPubMedCentralGoogle Scholar
- Mujeeb-Kazi A, Rosas V, Roldan S (1996) Conservation of the genetic variation of Triticum tauschii (Coss.) Schmalh. (Aegilops squarrosa auct. non L.) in synthetic hexaploid wheats (T. turgidum L. s.lat. x T. tauschii; 2n = 6x = 42, AABBDD) and its potential utilization for wheat improvement. Genet Res Crop Evol 43:129–134CrossRefGoogle Scholar
- Murai K, Ikari C, Shitsukawa N (2005) Pathways that promote the floral transition in wheat. In: Tsunewaki K (ed) Frontiers of wheat bioscience, Memorial Issue of Wheat Information Service. Kihara Memorial Foundation, Yokohama, pp 119–128Google Scholar
- Tsunewaki K (1966) Comparative gene analysis of common wheat and its ancestral species. II. Waxiness, growth habit and awnedness. Jpn J Bot 19:175–229Google Scholar
- Van Slageren MW (1994) Wild wheats: A monograph of Aegilops L. and Amblyopyrum (Jaub. & Spach) Eig (Poaceae). Wageningen Agricultural University, Wageningen, pp 326–344Google Scholar
- Wang S, Basten CJ, Zeng ZB (2011) Windows QTL cartographer 2.5. Department of Statics, North Carolina State University, Raleigh, NC. http://statgen.ncsu.edu/qtlcart/WQTLCart.htm