Genetic variation and association mapping of aphid (Macrosiphoniella sanbourni) resistance in chrysanthemum (Chrysanthemum morifolium Ramat.)
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Aphid, Macrosiphoniella sanbourni, is a major insect pest that adversely affects ornamental quality and production of chrysanthemum, thus it is critical to develop new cultivars resistant to aphid. However, the genetic mechanism governing aphid resistance is thus far not thoroughly investigated in chrysanthemum. This study aimed to characterize the genetic variation of the aphid resistance in a global collection of 80 chrysanthemum entries, during summer and autumn under greenhouse condition, and to identify the molecular markers for aphid resistance by association mapping. The performances of aphid resistance, quantified by the average damage index of aphid, was significantly correlated (r = 0.93, P < 0.01) between two seasons. The coefficients phenotypic and genetic variation was calculated around 26–27%; and a high magnitude (0.93) of broad-sense heritability, together with a moderate relative genetic advance (~ 68%), was estimated for aphid resistance. By using the MLM model that integrates population structure and kinship matrix as covariates association mapping identified 11 markers related to aphid resistance, with the individually explained phenotypic variation ranging from ~ 11 to ~ 57%. Of the three markers predicted in both seasons, SSR184-1 and E1M5-1were identified as favorable alleles for aphid resistance. Seven cultivars harboring the two favorable alleles were identified as potential donor parents for future improvement of resistance against aphid. These findings add further understanding of the genetic determination of aphid resistance, and the identified favorable alleles and donor parents open a possibility to produce chrysanthemums with enhanced aphid resistance in future.
KeywordsAphid resistance Association mapping Chrysanthemum Favorable allele Genetic variation
This work was financially supported by the National Natural Science Foundation of China (Grant Nos. 31471900 and 31672192). Germplasm Resources Protection (crop) project of Ministry of Agriculture (Grant No. 1120162130135252031).
- Davies FT, He C, Chau A, Heinz KM, Cartmill AD (2004) Fertility affects susceptibility of chrysanthemum to cotton aphids: influence on plant growth, photosynthesis, ethylene evolution, and herbivore abundance. J Am Soc Hortic Sci 129(3):344–353Google Scholar
- Deng Y, Chen S, Lu A, Chen F, Tang F, Guan Z, Teng N (2010) Production and characterization of the interneneric hybrids between Dendranthema morifolium and Artemisia vulgaris exhibiting enhanced resistance to chrysanthemum aphid (Macrosiphoniella sanbourni). Planta 231(3):693–703CrossRefPubMedGoogle Scholar
- He J, Chen F, Chen S, Fang W (2010) Aphid-resistance of chrysanthemum cultivars. Chinese J Ecol 29(7):1382–1386Google Scholar
- Meihls LN, Handrick V, Glauser G, Barbier H, Kaur H, Haribal MM, Lipka AE, Gershenzon J, Buckler ES, Erb M, Köllner TG, Jander G (2013) Natural variation in maize aphid resistance is associated with 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one glucoside methyltransferase activity. Plant Cell 25(6):2341–2355CrossRefPubMedPubMedCentralGoogle Scholar
- Pritchard JK, Falus D (2009) Documentation for STRUCTURE software: version 2.3. The University of Chicago Press, ChicagoGoogle Scholar
- Sun Y, Xia X, Jiang J, Chen S, Chen F, Lv G (2016) Salicylic acid-induced changes in physiological parameters and genes of the flavonoid biosynthesis pathway in Artemisia vulgaris and Dendranthema nankingense during aphid feeding. Genet Mol Res 15(1):gmr.15017546Google Scholar
- Suvija NV, Suresh J (2016) Evaluation of chrysanthemum (Chrysanthemum morifolium Ramat.) genotypes for loose flower, cut flower and pot mums. Int j innov res adv stud (IJIRAS) 3(4):100–104Google Scholar